Type Gene1 Effect Gene2 Sentence MedLine Reference Time of Interaction in evidence # of References territories of interaction Evidence for Direct Last author Journal Year start page end page
Expression activin positive chd Since activin is known to induce chordin transcription (Sasai et al., 1994), we tested whether Chordin was required for the dorsalization of animal cap explants by activin.
2 2 Public



positive
Expression activin positive fgf8a We demonstrate that transcription of fgf8a is induced by activin and the fgf8a protein inhibits the expression of nodal and pitx2a and leads to expression of the chicken snail related gene (cSnR) [9]. 10074453:7 2 3 Public



positive
Expression activin positive gata5 Injection of RNA encoding a constitutively active activin receptor leads to ectopic expression of gata5 and ntl. gata5 is activated cell-autonomously, whereas ntl is induced in cells distant from those which have received the RNA, showing that although ex 10375499:6 2 1 Public



positive
Expression activin positive gsc In these cells, activin rapidly induces the expression of the immediate early response genes goosecoid and no tail. 10349616:2 2 10 Public



positive
Expression activin positive lhx1a In this study, we report studies on the mechanism of regulation of the Lim-1 gene by activin/nodal signals and the role of FAST-1/FoxH1 in this process.
2 5 Public



positive
Expression activin positive myf5 activin treatment induced the expression of the somite marker genes Myf5 and Actin and the pan-neural marker NCAM.
2 1 Public



positive
Regulation activin positive ntl In Xenopus animal caps, Acti vin can induce gsc at high concentrations and Brachyury ( Xbra ), a marker for the notochord and posterior mesoderm, at lo wer concentrations (Green et al., 1997).
2 6 Public



positive
Expression activin positive pitx2a In contrast, both Xnr1 and activin injection resulted in strong acti v ation of pitx2a transcription in animal cap e xplants (Fig. 7), in agreement with the results obtained in injected whole embryos (Fig. 6B,C).
2 1 Public



positive
Expression activin positive smad2 Furthermore, although Madr2 remains unlocalized in ectodermal explants, addition of activin enhances the concentration of Madr2 in the nucleus. 8756346:4 2 3 Public



positive
Expression activin positive tbx6 Second, the mesoderm-inducing factors activin and bFGF activate tbx6 expression in animal caps. 9119115:4 2 2 Public



positive
Binding activin neutral acvr2a Inhibin also binds to ActRII through its beta subunit, competes with the binding of activin to ActRII, but fails to form the ActRII.SKR2 complex. 7890768:6 2 20 Public
HepG 2



Binding activin neutral acvr2a Inhibin also binds to ActRII through its beta subunit, competes with the binding of activin to ActRII, but fails to form the ActRII.SKR2 complex. 7890768:6 2 20 Public
HepG 2



Binding activin neutral bmp2a In this study, we have exploited the well characterized model of Xenopus mesoderm induction to determine the intracellular interactions between BMP-2/4 and activin/BVg1 signaling cascades. 9409665:3 2 3 Public





Binding activin neutral bmp3 A role for a BMP ligand acting as a BMP antagonist has been reported for the more distantly related BMP3, which binds to the BMP and activin receptor ActRII without activating it, thus acting as a receptor inhibitor (Daluiski et al., 2001 and Gamer et al.
2 1 Public





Binding activin neutral bmp7 BMP-7 can interact with activin type I and type II receptors in addition to BMP-2/BMP-4 receptors ( 26 , 28 ). 9268344:10043 2 5 Public





Binding activin neutral foxh1 This motif is also located within the SID domain of FoxH1 and is conserved in all family members, suggestingthat this region may also be critical for FoxH1 association with the TGF?/activin regulated Smads.
2 4 Public





Binding activin neutral fsta Follistatin (FS) binds activin and inhibin proteins. 9858480:0 2 86 Public
Hs



Expression activin neutral gsc Goosecoid is a homeobox gene that is expressed as an immediate early response to mesoderm induction by activin. 8734495:0 2 1 Public



unknown
Binding activin neutral nog1 Furthermore, their binding to BMP-2 differs from the binding mode established for noggin/BMP7 or follistatin/activin interaction.
2 2 Public





Binding activin neutral smad The FAST/Smad complex binds to activin/nodal responsive elements (ARE/NRE) and activates homeobox genes implicated in mesoderm induction in both Xenopus and mouse (reviewed in Massague and Wotton, 2000).
2 1 Public





Binding activin neutral smad2 A paradigm of Smad–cofactor interaction was established for TGF-?/activin signaling by the identification of winged helix proteins, such as FAST-1 as DNA-binding partners that interact with Smad2/3 at target promoters (14,15).
2 1 Public





Binding activin neutral smad3a Among them, Smad3 is shown to transiently associate with activin type IB and TGF-eta type I receptor and to be phosphorylated by the type I receptors to propagate downstream signaling ( 29 ). 10681527:10154 2 3 Public





Binding activin neutral tgfb1 Ongoing experiments will explore the nature of the interaction between activin and TGF-beta in detail, by testing some of these hypotheses. 9275089:10280 2 2 Public





Binding activin neutral wnt Melton, Interaction of Wnt and activin in dorsal mesoderm induction in Xenopus.
2 3 Public





Expression acvr1 positive bmp2a Since Bmps act as negative regulators of chordin expression (Schulte-Merker et al., 1998), and CA and KM Alk8 affect the expression of bmps2b and -4, it is expected that chordin expression would also be affected.
2 2 Public



positive
Expression acvr1 positive bmp2b injected mRNA encoding a constitutively active version of Alk8 can rescue the strong dorsalization of bmp2b/swirl mutants. overexpression 4.66 mesendoderm blastoderm 2 Hammerschmidt, M. Development 2001 128 849
Regulation acvr1 positive bmp4 A constitutively active Alk8-TGFbeta-receptor can ectopically induce bmp2b and bmp4 and rescues the dorsalization of MZspg. 16775002:9 2 1 Public

zygote
positive
Expression acvr1 positive bmp4 This indicates that pou2 acts upstream of Alk8, a maternally provided receptor implicated in the activation of zygotic bmp2b and bmp4 transcription. 16775002:10 2 16 Public

zygote
positive
Expression acvr1 positive bmp7 injected mRNA encoding a constitutively active version of Alk8 can rescue the strong dorsalization of bmp7/snailhouse mutants overexpression 4.66 mesendoderm blastoderm 2 Hammerschmidt, M. Development 2001 128 849
Regulation acvr1 positive bre Furthermore, Smad7 inhibited the expression of the BMP-responsive reporter BRE-Luc (18) induced by the constitutively active forms of BMPRIA, BMPRIB, acvrl1, and ALK2 (Fig. 1B).
2 4 Public



positive
Expression acvr1 positive bre Furthermore, Smad7 inhibited the expression of the BMP-responsive reporter BRE-Luc (18) induced by the constitutively active forms of BMPRIA, BMPRIB, acvrl1, and ALK2 (Fig. 1B).
2 4 Public



positive
Expression acvr1 positive chd By early somite stages, CA Alk8 expressing embryos exhibit reduced chordin expression in diminished anterior neural structures and in the ventral tailbud (Fig. 2U,U').
2 2 Public



positive
Expression acvr1 positive dlx3b in alk8 (a type I TGFbeta receptor) MO-injected embryos, vhnf1 expression in the endoderm is significantly reduced. mutant embryos 8 epiblast ectoderm 1 Mary C. Mullins Development 2001 128 859
Expression acvr1 positive foxa3 Decrease of Bmp activity by alk8 morpholino injection caused a change of foxa3 expression pattern.
2 1 Public



positive
Expression acvr1 positive gata2 the data suggest that Alk8 acts as a Bmp2b/7 receptor upstream of Smad5. mutant embryos 8 epiblast ectoderm 1 Mary C. Mullins Development 2001 128 859
Expression acvr1 positive hnf1b Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI signaling mutants. MASO 24 gut gut 3 Lin, S. Dev Biol 2007 303 561
Expression acvr1 positive myod1 CA Alk8 expressing embryos often display an interrupted myoD expression pattern, where adaxial cells appaear fused, forming a loop toward the posterior end.
2 2 Public



positive
Expression acvr1 positive otx2 At 80% epiboly, CA Alk8 expressing embryos exhibit restricted dorsal anterior otx2 expression (Fig. 5C,C'), while KM Alk8 expressing embryos exhibit up regulated midline and lateral expansion of otx2 expression (Fig. 5A,A').
2 2 Public



positive
Expression acvr1 positive smad5 The ability of sbn/Smad5 mutants to block the ventralizing activities of CA alk8 demonstrate that alk8 functions upstream of the Smad5 transcription factor, and suggests that Smad5 is required in alk8 signaling pathways.
2 2 Public



positive
Expression acvr1 positive smad5 In most MZ-laf/alk8 mutants dlx3 expression is absent. MASO 4.66 mesendoderm blastoderm 1, 2 Hammerschmidt, M. Development 2001 128 849
Expression acvr1 positive spi1 Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI signaling mutants. mutant embryos 11.66 lateral plate mesoderm lateral plate mesoderm 1 Lieschke, G. J. Curr Biol 2006 16 506
ProtModification acvr1 phosphorylation smad1 B, in vitro phosphorylation of Smad1 by ALK2. 9748228:10235 2 15 Public
HepG 2

unknown
ProtModification acvr1 phosphorylation smad1 The activated BMP type I receptors acvrl1, ALK2, ALK3 and ALK6 phosphorylate downstream target Smads 1/5/8 ([Heldin et al., 1997] and [Massague and Wotton, 2000]) which then bind to Smad4, migrate to the nucleus and regulate transcription of target genes
2 4 Public



unknown
ProtModification acvr1 phosphorylation smad5 When stimulated by Bmp7, Alk2 recognizes and phosphorylates Smad1, and also phosphorylates Smad5 and Smad8 ( Macias-Silva et al., 1998).
2 7 Public



unknown
ProtModification acvr1 phosphorylation smad8 Constitutively active BMP type I receptors, ALK-3 and ALK-6, as well as ALK-2, were phosphorylated Smad8 and induced Smad8 interaction with Smad4. 10814522:3 2 8 Public
COS I osteoblast
unknown
Expression acvr1 neutral bmp4 Together, these results show that Mrdr and alk8 strongly interact to positively regulate the onset of bmp2b/4 expression.
2 3 Public



unknown
Binding acvr1 neutral bmp7 OP-1 bound to ALK-2 and ALK-6 efficiently, and to ALK-3 less efficiently, whereas BMP-4 bound to ALK-3 and ALK-6 efficiently. 8006002:6 2 21 Public
COS osteoblast


Expression acvr1 neutral bre Furthermore, Smad7 inhibited the expression of the BMP-responsive reporter BRE-Luc (18) induced by the constitutively active forms of BMPRIA, BMPRIB, acvrl1, and ALK2 (Fig. 1B).
2 4 Public



unknown
Expression acvr1 neutral chd By early somite stages, CA Alk8 expressing embryos exhibit reduced chordin expression in diminished anterior neural structures and in the ventral tailbud (Fig. 2U,U').
2 2 Public



unknown
Expression acvr1 neutral myod1 KM Alk8 expression results in embryos exhibiting normal adaxial myoD expression along a broadened midline and thickened somitic myoD expression.
2 2 Public



unknown
Expression acvr1 neutral otx2 At 80% epiboly, CA Alk8 expressing embryos exhibit restricted dorsal anterior otx2 expression (Fig. 5C,C'), while KM Alk8 expressing embryos exhibit up regulated midline and lateral expansion of otx2 expression (Fig. 5A,A').
2 2 Public



unknown
Binding acvr1 neutral smad1 A, ALK2 interacts with Smad1. 9748228:10229 2 6 Public





Binding acvr1 neutral smad4 The activated BMP type I receptors acvrl1, ALK2, ALK3 and ALK6 phosphorylate downstream target Smads 1/5/8 ([Heldin et al., 1997] and [Massague and Wotton, 2000]) which then bind to Smad4, migrate to the nucleus and regulate transcription of target genes
2 6 Public





Binding acvr1 neutral smurf2 In addition, Smurf1 associates with transforming growth factor-beta type I receptor through the inhibitory Smad (I-Smad) Smad7 and induces their degradation. 12857866:1 2 11 Public





Binding acvr1 neutral tgfb1 When coexpressed with the type II receptors of TGF-eta and activin, ALK-2 was shown to be able to form a heteromeric complex with TGF-eta and activin, respectively ( 7-9 ). 9371779:10208 2 5 Public
nmumg



Expression acvr1 negative bmp4 In contrast, KM Alk8 down regulates ventral bmp2b expression in shield stage embryos (data not shown).
2 2 Public



negative
Expression acvr1 negative chd Ectopic expression of CA Alk8 reduces chordin expression in shield and 80% epiboly stage embryos (Fig. 2O,R), while KM Alk8 expands chordin expression (Fig. 2M,P).
2 2 Public



negative
Expression acvr1 negative foxb1.2 the data suggest that Alk8 acts as a Bmp2b/7 receptor upstream of Smad5. mutant embryos 8 epiblast ectoderm 1 Mary C. Mullins Development 2001 128 859
Expression acvr1 negative ins Embryos injected with alk8 morpholino exhibited remarkably reduced insulin expression (Fig. 8F).
2 1 Public



negative
Expression acvr1 negative otx2 Otx2 expression is reduced by CA Alk8 and expanded by KM Alk8, demonstrating a respective loss and expansion of prospective neural tissue that is appaarent at very early stages of development.
2 2 Public



negative
Expression acvr1 negative ved Foxb1.2 expression is expanded ventrally in MZ-laf embryos at 70% epiboly, the expansion is more pronounced in more vegetal regions. mutant embryos 4.66 EVL blastoderm 1 Franco Cotelli Developmental Dynamics 2004 230 494
Binding acvr1b neutral acvr2a If cotransfected with ActRII/IIB and ALK4, Cripto inhibited crosslinking of activin to ALK4 and the association of ALK4 with ActRII/IIB. 12682303:6 2 13 Public
nmumg keratinocyte


Binding acvr1b neutral acvr2a If cotransfected with ActRII/IIB and ALK4, Cripto inhibited crosslinking of activin to ALK4 and the association of ALK4 with ActRII/IIB. 12682303:6 2 13 Public
nmumg keratinocyte


Expression acvr1b positive ntl Injection of TARAM-A* (acvr1b) induced very robust expression of ntl in large patches of cells. overexpression 4.33 margin margin 2 Thierry Lepage Development 2002 129 4901
Expression acvr1b positive og9x Injection of TARAM-A*(acvr1b) induced very robust expression of casanova (sox32) in large patches of cells. overexpression 5.25 margin margin 2 Thierry Lepage Development 2002 129 4901
Expression acvr1b positive pitx2a We report here that similar to effects of ectopic TGF betas, right-side expression of constitutively active activin-like kinase (ALK) 4 results in LR organ reversals as well as altered pitx2a expression in the lateral plate mesoderm. 15063168:2 2 3 Public
LR cardiomyocyte
positive
ProtModification acvr1b phosphorylation smad The binding of Nodal to its receptor complex (Alk4/ActRIIB/co-receptor EGF-CFC) activates Smad2/3, and phosphorylation of these Smads by Alk4 increases their affinity for Smad4.
2 1 Public



unknown
Binding acvr1b neutral smad The ?4-?5 loop of TaramA, a Smad interacting domain, is identical to that of Alk4 ( ten Dijke et al., 1994b), suggesting that TaramA and Alk4 may interact with similar downstream Smad molecules and may perform similar functions.When CA alk8 and taramA* RN
2 2 Public





ProtModification acvr1b phosphorylation smad2 This suggests that Smad2 and Smad3 association with ALK4 is an early event after receptor heteromerization, in activin signaling. 9892009:1092 2 5 Public
293

unknown
Expression acvr1b positive smad2 Ca-ALK4 induced a strong nuclear accumulation of EGFP-Smad2 during the 20 min washout period, and this was completely abolished by the treatment with AA (Figure 4A).
2 1 Public



positive
ProtModification acvr1b phosphorylation smad3a ALK4 is a Ser/Thr kinase that phosphorylates cytoplasmic Smad2 and Smad3 ( Massague and W otton, 2000 ).
2 6 Public



unknown
Binding acvr1b neutral smad3a Nevertheless, when cells were stimulated by activin, the amount of Smad3 associated with ALK4 increases. 9892009:1073 2 3 Public





Expression acvr1b positive sox17 Similarly, whereas injection of very low doses 1-2 pg of tar*/acvr1b caused the ectopic expression of sox17 in about 32% of the embryos, treatment with SU5402 following the injection increased this percentage to 54% (Table 1).
2 9 Public



positive
Expression acvr1b positive sox17 Injection of TARAM-A* induced very robust expression of sox17, ntl,og9x, bon/mixer and casanova in large patches of cells. overexpression 4.33 margin margin 2 Thierry Lepage Development 2002 129 4901
Expression acvr1b positive sox32 Embryo injected at the eight-cell stage into one blastomere with 40 pg of TARAM-A*(acvr1b) and with 100 pg of mRNA encoding a nuclear b-Gal (blue colour) as a lineage tracer. TARAM-A*(acvr1b) induces confluent patches of mezzo (og9x)-expressing cells. overexpression 4.33 margin margin 2 Thierry Lepage Development 2002 129 4901
Regulation acvr1b unknown tgfb1 This phenomenon was also observed in MZoep mutant embryos treated with the small compound SB431542 (39), which specifically inhibits ALK4/5/7-mediated TGF-? signaling.
2 1 Public



unknown
Binding acvr1b neutral tgfb1 Together, these results suggest not only that ALK4 interacts with multiple TGF?s to generate embryonic pattern, but also that ALK4 ligands differentially utilize the ALK4 pathway to regulate distinct aspects of axial pattern, with Vg1 as a modulator of AL
2 1 Public





ProtModification acvr1b phosphorylation ttrap ALK4 phosphorylated TTRAP in vitro (Fig. 6A).
2 1 Public



unknown
Binding acvr1b neutral ttrap Weobserved binding of Alk4 to TTRAP (Fig. 5D).
2 1 Public





Binding acvr2b neutral bmp2a Consistent with previous observations on the Drosophila type II receptor, punt ( 56 ), we were unable to observe any binding of BMP-2 to ActRII (Fig. 7 A , lane 8 ) or ActRIIB (data not shown) when these receptors were expressed alone. 9872992:10251 2 5 Public





Binding acvr2b neutral bmpr1a Moreover, ActRIIB can recruit both Alk4 for activin-like signaling and Alk3 for BMP-like signaling (Massague and Chen 2000 ). 3240211037 2 2 Public





Regulation acvr2b negative lft2 Similarly, the effects of Antivin and Lefty2 can be suppressed by overexpression of the nodal-related genes cyclops and squint or the extracellular domain of ActRIIB. 10518210:4 2 8 Public



negative
Regulation acvrl1 positive bre Furthermore, Smad7 inhibited the expression of the BMP-responsive reporter BRE-Luc (18) induced by the constitutively active forms of BMPRIA, BMPRIB, acvrl1, and ALK2 (Fig. 1B).
2 4 Public



positive
Expression acvrl1 neutral bre Furthermore, Smad7 inhibited the expression of the BMP-responsive reporter BRE-Luc (18) induced by the constitutively active forms of BMPRIA, BMPRIB, acvrl1, and ALK2 (Fig. 1B).
2 4 Public



unknown
Expression acvrl1 positive bre Furthermore, Smad7 inhibited the expression of the BMP-responsive reporter BRE-Luc (18) induced by the constitutively active forms of BMPRIA, BMPRIB, acvrl1, and ALK2 (Fig. 1B).
2 4 Public



positive
Regulation acvrl1 positive smad1 TGF-beta/acvrl1-induced Smad1/5 phosphorylation in ECs occurs transiently. 16571110:3 2 20 Public
ec

positive
ProtModification acvrl1 phosphorylation smad1 The activated BMP type I receptors acvrl1, ALK2, ALK3 and ALK6 phosphorylate downstream target Smads 1/5/8 ([Heldin et al., 1997] and [Massague and Wotton, 2000]) which then bind to Smad4, migrate to the nucleus and regulate transcription of target genes
2 5 Public



unknown
Binding acvrl1 neutral smad1 For the interaction of Smad1 with ALK-1 or ALK-2 not only the L3 loop is required, but also the alpha-helix 1 (H1) in MH2 domain [ 22 ]. 11106403:10085 2 5 Public





Binding acvrl1 neutral smad4 The activated BMP type I receptors acvrl1, ALK2, ALK3 and ALK6 phosphorylate downstream target Smads 1/5/8 ([Heldin et al., 1997] and [Massague and Wotton, 2000]) which then bind to Smad4, migrate to the nucleus and regulate transcription of target genes
2 6 Public





ProtModification acvrl1 phosphorylation smad5 The activated BMP type I receptors acvrl1, ALK2, ALK3 and ALK6 phosphorylate downstream target Smads 1/5/8 ([Heldin et al., 1997] and [Massague and Wotton, 2000]) which then bind to Smad4, migrate to the nucleus and regulate transcription of target genes
2 5 Public



unknown
ProtModification acvrl1 phosphorylation smad8 The activated BMP type I receptors acvrl1, ALK2, ALK3 and ALK6 phosphorylate downstream target Smads 1/5/8 ([Heldin et al., 1997] and [Massague and Wotton, 2000]) which then bind to Smad4, migrate to the nucleus and regulate transcription of target genes
2 5 Public



unknown
Regulation acvrl1 unknown tgfb1 Endothelial TGF-beta pathways, mediated by acvrl1 and ALK5, are impaired in HHT cells. 15993872:5 2 11 Public



unknown
Expression acvrl1 positive tlx2 Expression of activated acvrl1 in P19 cells induced the Tlx2 promoter to a level similar to that observed for ALK2 (Fig. 8 B ), whereas activated acvrl1 was unable to induce the activin-responsive reporter gene, pA3-lux (data not shown). 9748228:10259 2 2 Public



positive
Expression bmp2a negative acvr1b overexpression of BMPs compromises endoderm formation, formation of endoderm precursors is negatively regulated by BMPs on the ventral side. overexpression 5.25 mesendoderm blastoderm 2 Thierry Lepage Development 2006 133 2189
Expression bmp2a negative ndr1 In embryos in which Bmp signaling was blocked at 18 hpf, gata4 and gata6 expression was greatly reduced in the liver region at 29-30 hpf. overexpression 8 margin margin 2 Hammerschmidt, M. Curr Biol 2007 17 475
Expression bmp2b negative acvr1b We never found a homozygous laftm110b embryo that had a wild-type or ventralized phenotype, consistent with the Laf/Alk8 receptor functioning downstream of the Bmp2b. overexpression 5.25 mesendoderm blastoderm 2 Thierry Lepage Development 2006 133 2189
Expression bmp2b negative ndr1 Ved expression domain expanded to the dorsal margin in bmp2b RNA-injected embryos at the 80% epiboly stage. overexpression 8 margin margin 2 Hammerschmidt, M. Curr Biol 2007 17 475
Regulation bmp7 positive acvr1 OP-1 alone increased ActR-I, BMPR-IA, and BMPR-II mRNA levels, but did not change the BMPR-IB level. 9322928:10188 2 4 Public



positive
Expression bmp7 negative acvr1b Prdm1 expression is reduced in snh (BMP7) mutant embryos. overexpression 5.25 mesendoderm blastoderm 2 Thierry Lepage Development 2006 133 2189
Expression bmp7 negative ndr1 Ved expression is down-regulated at late blastula stage in snh/bmp7 mutants. overexpression 8 margin margin 2 Hammerschmidt, M. Curr Biol 2007 17 475
Expression calr neutral ndr2 During gastrulation, calr eticulin transcripts are found in the dorsal mesendoderm, in the same cells that express the cyc gene. 10660676:6 2 2 Public



unknown
Expression ctnnb1 positive ndr1 Injection of b-catenin RNA elicited the ectopic expression of boz/dharma at the sphere and shield stages overexpression 4.66 margin margin 2 Hirano, T. Mechanisms of Development 2000 91 293
Expression ctnnb1 positive ndr1 At the onset of zygotic transcription, eta-catenin activates expression of nodal -related genes together with the homeobox genes bozozok / dharma / nieuwkoid ( bozozok ) in zebrafish, and siamois , twin in Xenopus ; these genes cooperatively promote organ 11124801:10016 2 3 Public



positive
Expression ctnnb1 positive ndr2 Overexpression of ?-catenin induces cyc expression at 6 h (A), but not at 4 h (E, sphere) or 5 h (C, 40% epiboly). (G-J) Analysis of gsc expression at 5 hours in embryos from a ndr1 +/- intercross injected with ?-catenin or lacZ mRNA. ?-catenin mRNA (G),
2 3 Public



positive
Expression ctnnb1 positive ndr2 At the onset of zygotic transcription, eta-catenin activates expression of nodal -related genes together with the homeobox genes bozozok / dharma / nieuwkoid ( bozozok ) in zebrafish, and siamois , twin in Xenopus ; these genes cooperatively promote organ 11124801:10016 2 3 Public



positive
Regulation eomesa unknown ndr1 This intriguing possibility was investigated further by animal pole injections, which indicated that eomes could modulate the induction of gsc and flh by ndr1 .
2 6 Public



unknown
Expression fgf17 negative acvr1b activation of the FGF/ERK pathway disrupts endoderm formation in the embryo and antagonizes the ability of an activated form of Tar/Acvr1b to induce endoderm at the animal pole. overexpression 5.25 mesendoderm blastoderm 2 Thierry Lepage Development 2006 133 2189
Expression fgf24 negative acvr1b Microinjection of fgf24 leads to inhibition of bmp2b, bmp4 and bmp7 expression at blastula stage. overexpression 5.25 mesendoderm blastoderm 2 Thierry Lepage Development 2006 133 2189
Expression fgf3 negative acvr1b Microinjection of fgf3 leads to inhibition of bmp2b expression at blastula stage. overexpression 5.25 mesendoderm blastoderm 2 Thierry Lepage Development 2006 133 2189
Expression fgf8 negative acvr1b The fgf8 overexpression (10 pg) induced chd at the shield stage. overexpression 5.25 mesendoderm blastoderm 2 Thierry Lepage Development 2006 133 2189
Expression foxh1 positive ndr1 FoxH1 is believed to drive transcription of the zebrafish Nodal-related genes and their antagonists, the lefties.
2 2 Public



positive
Expression foxh1 positive ndr2 FoxH1 is believed to drive transcription of the zebrafish Nodal-related genes and their antagonists, the lefties.
2 2 Public



positive
Expression hsp90a.1 positive ndr2 By extension, perhaps variations in genetic background, cold treatment and loss of HSP90 function also affect ndr1 penetrance via reductions in cyc transcription.
2 2 Public



positive
Expression lft2 negative ndr2 The phenotypic effects induced by overexpression of Antivin or Lefty2 might be caused by blocking Nodal signaling and/or by blocking expression of cyc and ndr1 .
2 1 Public



negative
Expression lft2 negative ndr2 axial expression is reduced in the midline and abolished in endodermal precursors of injected embryos. overexpression 8 mesoderm mesoderm 2 H. Joseph Yost Development 1999 126 3253
Regulation mapk1 negative acvr1b FGF/ERK activity antagonizes the ability of tar*/acvr1b to induce endoderm.
2 8 Public



negative
Expression myod1 positive oep Formation of eye muscles doesn't form in oep mutant embryos at 42 hpf as judged from expression of myoD. mutant embryos 42 eye muscles musculature 1 Wolfgang Driever Development 1997 124 327
Expression ndr1 positive ndr1 Maternal Ndr1 and Ndr2 mutant has normal expression of hgg1, a marker for anterior dorsal mesoderm, detected by whole-mount in situ hybridization in 10-somite-stage embryos. MASO 0.5 blastomere blastomere 3 Sampath, K. Nature 2005 438 1030
Expression ndr1 neutral ndr1 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
2 3 Public



unknown
Expression ndr1 positive ndr1 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
2 3 Public



positive
Expression ndr1 positive ndr2 Maternal Ndr1 and Ndr2 mutant has normal expression of hgg1, a marker for anterior dorsal mesoderm, detected by whole-mount in situ hybridization in 10-somite-stage embryos. MASO 0.5 blastomere blastomere 3 Sampath, K. Nature 2005 438 1030
Expression ndr1 neutral ndr2 Therefore, Cyc and ndr1 function as redundant signals that modulate cyc and pitx2a transcription in the dorsal forebrain.
2 2 Public



unknown
Expression ndr1 neutral ndr2 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
2 3 Public



unknown
Expression ndr1 positive ndr2 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
2 3 Public



positive
Expression ndr1 negative bmp4 11291860 [PubMed - indexed for MEDLINE]Related ArticlesThe homeobox gene bozozok promotes anterior neuroectoderm formation in zebrafish through negative regulation of BMP2/4 and Wnt pathways. [Development. 2000] Cooperative roles of Bozozok/Dharma and Nod
2 3 Public



negative
Expression ndr1 positive bon overexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed mutant embryos 5.25 margin margin 1 Derek L. Stemple Current Biology 1999 9 1147
Expression ndr1 positive chd In sqt mutants, the bon expression domain is thinner along the animal/vegetal axis and exhibits a dorsal gap. Strikingly, cyc;sqt double mutants exhibit a barely detectable level of bon expression. overexpression 0 DEL blastoderm 1, 2 Hirano, T. Mechanisms of Development 2000 91 293
Expression ndr1 neutral dact1 Thus, Boz, ndr1 , and Cyc are all capable of inducing dpr1 and dpr2 gene expression.
2 1 Public



unknown
Expression ndr1 positive dact1 Thus, Boz, ndr1 , and Cyc are all capable of inducing dpr1 and dpr2 gene expression.
2 1 Public



positive
Expression ndr1 positive dact1 The dorsalizing factors beta-catenin, Bozozok (Boz), Noggin (Nog), and the mesendoderm-inducing factor Squint (ndr1 ) are all able to induce ectopic expression of dpr1 and dpr2. 15765513:5 2 3 Public



positive
Expression ndr1 positive dkk1 overexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed mutant embryos 5.25 mesendoderm blastoderm 1 Didier Y. R. Stainier Development 2003 130 4989
Expression ndr1 positive fgf17 overexpression of sqt elicited the ectopic expression of chd in the entire blastoderm at the sphere to shield stage. In the sqt mutant embryos din was expressed at reduced levels throughout the sphere and shield stages. overexpression 4 YSL YSL 2 Anming Meng Developmental Biology 2004 271 130
Regulation ndr1 positive flh This intriguing possibility was investigated further by animal pole injections, which indicated that eomes could modulate the induction of gsc and flh by ndr1 .
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positive
Expression ndr1 positive foxa2 These results show that dorsal cyc expression is activated by sqt at 5 h, the first stage where a reduction of cyc expression is evident in sqt mutants. The reduction of cyc expression in sqt–/–; cyc+/– embryos when compared with sqt–/–; cyc+/+ mutants su mutant embryos 6 mesendoderm blastoderm 1 William S. Talbot Development 2003 130 1837
Expression ndr1 positive gsc Thus overexpression of Ndr1/2 is sufficient to induce chd expression. But analysis of boz mutants and single and double mutants for the nodal-related genes shows that they are not essential for chd expression in the late blastula. overexpression 4 YSL YSL 1, 2 Hirano, T. Mechanisms of Development 2000 91 293
Expression ndr1 positive ndr1 Maternal Ndr1 and Ndr2 mutant has normal expression of hgg1, a marker for anterior dorsal mesoderm, detected by whole-mount in situ hybridization in 10-somite-stage embryos. MASO 0.5 blastomere blastomere 3 Sampath, K. Nature 2005 438 1030
Expression ndr1 neutral ndr1 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
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unknown
Expression ndr1 positive ndr1 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
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positive
Expression ndr1 positive ndr2 Maternal Ndr1 and Ndr2 mutant has normal expression of hgg1, a marker for anterior dorsal mesoderm, detected by whole-mount in situ hybridization in 10-somite-stage embryos. MASO 0.5 blastomere blastomere 3 Sampath, K. Nature 2005 438 1030
Expression ndr1 neutral ndr2 Therefore, Cyc and ndr1 function as redundant signals that modulate cyc and pitx2a transcription in the dorsal forebrain.
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unknown
Expression ndr1 neutral ndr2 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
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unknown
Expression ndr1 positive ndr2 The loss of gfp and ndr1 from the YSL could be due to decreased expression of an inducer of nodal-related gene expression, or to increased expression of an inhibitor.
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positive
Expression ndr1 positive ntl overexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expresse overexpression 5.25 mesendoderm blastoderm 2 Schier, A. F. Development 2003 130 5589
Expression ndr1 positive ntn1b 10952887 [PubMed - indexed for MEDLINE]Related ArticlesA temperature-sensitive mutation in the nodal-related gene cyclops reveals that the floor plate is induced during gastrulation in zebrafish. [Development. 2003] Two distinct cell populations in the fl
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positive
Expression ndr1 positive og9x overexpression of sqt (ndr1) elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was ex mutant embryos 5.25 margin margin 1 Thierry Lepage Development 2002 129 4901
Expression ndr1 positive otx2 Injection with 1pg sqt or 10 pg of fgf17b mRNA caused ectopic expression of spr2. mutant embryos 10 neuronectoderm neuroectoderm 1 Didier Y. R. Stainier Development 2003 130 4989
Expression ndr1 neutral pitx2a Therefore, Cyc and ndr1 function as redundant signals that modulate cyc and pitx2a transcription in the dorsal forebrain.
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unknown
Expression ndr1 positive snai1a overexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed overexpression 5.25 margin margin 2,4 Anming Meng The EMBO Journal 2003 22 6078
Expression ndr1 positive sox17 Almost all of the embryos injected with 0.5 pg sqt mRNA alone showed a large increase in ntl expression in the axial mesoderm. mutant embryos 6 mesendoderm blastoderm 1 William S. Talbot Development 2003 130 1837
Expression ndr1 positive sox32 By contrast, in squint mutant embryos, the expression domain of og9x was thinner along the animalvegetal axis and displayed a gap. overexpression 6 YSL YSL 2 Hiroyuki Takeda Mechanisms of Development 2001 107 25
Expression ndr1 positive sp5l overexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed overexpression 5.25 margin margin 2 Anming Meng The EMBO Journal 2003 22 6078
Expression ndr2 positive ndr1 The expression patterns of ndr1 and cyc are consistent with an elevated dorsal requirement for nodal-related gene function.
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positive
Expression ndr2 positive ndr2 The expression patterns of ndr1 and cyc are consistent with an elevated dorsal requirement for nodal-related gene function.
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positive
Expression ndr2 positive ndr2 Dorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh Injections of higher doses of cyc+ R mutant embryos 5.25 margin margin 2 Scott T. Dougan1 Development 2003 130 1837
Expression ndr2 negative bmp4 11291860 [PubMed - indexed for MEDLINE]Related ArticlesThe homeobox gene bozozok promotes anterior neuroectoderm formation in zebrafish through negative regulation of BMP2/4 and Wnt pathways. [Development. 2000] Cooperative roles of Bozozok/Dharma and Nod
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negative
Regulation ndr2 positive chd Cyc consistently induced mesodermal (Xbra, Xwnt8, actin), organizer (chordin) and neural (NCAM) markers.
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positive
Expression ndr2 neutral dact1 Thus, Boz, ndr1 , and Cyc are all capable of inducing dpr1 and dpr2 gene expression.
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unknown
Expression ndr2 positive dact1 Thus, Boz, ndr1 , and Cyc are all capable of inducing dpr1 and dpr2 gene expression.
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positive
Expression ndr2 positive ehhadh The nodal-related gene cyclops is not required for floor plate development in the absence of tbx1 6 gene function Previous work has shown that the nodal-related gene cyclops (cyc) is required for MFP formation (Hatta et al., 1991; Rebagliati et al., 1998
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positive
Expression ndr2 negative emx1 Sirotkin and others Fig . 9. cyc represses anterior neural fates. (A-H) Expression of emx1 (most anterior label) and kr ox20 (R3 and R5 label) in embryos at the 2-somite stage (10.7 h).
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negative
Expression ndr2 positive flh Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh. Injections of higher doses of cyc+ RNA result in expansion of the gsc and twhh doma overexpression 5.25 margin margin 2 Schier, A. F. Development 2000 127 921
Expression ndr2 positive foxa2 In 5 somite cyc -/ -embryos, zp-50 is expressed in rhombomeres r3 and r5 and the transverse domain in the posterior diencephalon. Arrowheads indicate missing zp-50 expression in the ventroanterior brain. mutant embryos 11.66 ventral neural tube neuroectoderm 1 P.W. Ingham GENES & DEVELOPMENT 1993 7 1436
Expression ndr2 positive gsc Overexpression of Antivin and Lefty2 inhibits the expression of the dorsal markers goosecoid and sonic hedgehog ([36]; Figure 5J and Figure 5P; data not shown) whereas overexpression of ndr1 or Cyc induces ectopic goosecoid and hedgehog expression ([38,
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positive
Expression ndr2 positive gsc Overexpression of cyclops and squint at different doses leads to the induction of floating head at low doses and the induction of both goosecoid (Gsc) and floating head (Flh) at higher doses overexpression 5.25 mesendoderm blastoderm 2 Schier, A. F. Development 2000 127 921
Expression ndr2 positive lft1 cyc mutant embryos do not express lft1 or lft2 in the anterior midline. mutant embryos