Expression Table

Gene Name: hoxb5a
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hoxb5a

Stage

Hours

ZFIN expression range

paraxial mesoderm

tail bud

neural rod

somite 2

somite 3

neural tube

pharyngeal arch

spinal cord

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction in kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence paraxial mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction In kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence paraxial mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction in kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence paraxial mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction In kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence paraxial mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction in kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction In kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction in kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction In kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction in kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction In kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction in kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction In kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.

Target Gene hoxb5a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction	in kgg tv205 mutants, hoxb5a expression is expanded to include somites two to five, is absent from the notochord and is reduced in the tailbud.
Target Gene	hoxb5a
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	paraxial mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18