Expression Table

Gene Name: hoxb4a
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hoxb4a

Stage

Hours

ZFIN expression range

presumptive rhombomere 7

presumptive rhombomere 8

central nervous system

somite 1

somite 2

somite 3

somite 4

somite 5

neural tube

rhombomere 7

rhombomere 8

hindbrain

spinal cord

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Hoxb4a display altered expression patterns in kgg tv209 embryos.

Target Gene hoxb4a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence CNS

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Hoxb4 display altered expression patterns in kgg tv205 embryos.

Target Gene hoxb4a

Upstream Gene cdx4

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence CNS

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Hoxb4 display altered expression patterns in kgg tv205 embryos.

Target Gene hoxb4a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction	Hoxb4a display altered expression patterns in kgg tv209 embryos.
Target Gene	hoxb4a
Upstream Gene	cdx4
Interaction Type	repress
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	CNS
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18

Evidence for Interaction	Hoxb4 display altered expression patterns in kgg tv205 embryos.
Target Gene	hoxb4a
Upstream Gene	cdx4
Interaction Type	repress
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	CNS
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18