Expression Table

Gene Name: hoxb1b
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hoxb1b

Stage

Hours

ZFIN expression range

presumptive brain

presumptive neural plate

germ ring

presumptive hindbrain

neural plate

neuroectoderm

hindbrain neural plate

neural keel

segmental plate

tail bud

notochord

lateral plate mesoderm

somite

neuron

neural tube

hindbrain

pharyngeal arch 3-7 skeleton

spinal cord

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Expressions of the neuroectodermal genes hoxa-1 was expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.

Target Gene hoxb1b

Upstream Gene zfhx1

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Naomasa Miki

Journal Developmental Biology

Year 2000

Vol 228

Start Page 29

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested Fgf17b mRNAs.

Target Gene hoxb1b

Upstream Gene fgf17b

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence ectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of hoxb1b expanded toward the animal pole in all the fgf17b injected embryos.

Target Gene hoxb1b

Upstream Gene fgf17b

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested Fgf17b mRNAs.

Target Gene hoxb1b

Upstream Gene fgf17b

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded, predominantly on the ventral side, by overexpression of any of the tested Fgf mRNAs.

Target Gene hoxb1b

Upstream Gene fgf3

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested fgf3 mRNAs.

Target Gene hoxb1b

Upstream Gene fgf3

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded, predominantly on the ventral side, by overexpression of any of the tested Fgf mRNAs.

Target Gene hoxb1b

Upstream Gene fgf8

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested fgf8 mRNAs.

Target Gene hoxb1b

Upstream Gene fgf8

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded, predominantly on the ventral side, by overexpression of any of the tested Fgf mRNAs.

Target Gene hoxb1b

Upstream Gene fgf3

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence presumptive brain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested fgf3 mRNAs.

Target Gene hoxb1b

Upstream Gene fgf3

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence presumptive brain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded, predominantly on the ventral side, by overexpression of any of the tested Fgf mRNAs.

Target Gene hoxb1b

Upstream Gene fgf8

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence presumptive brain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested fgf8 mRNAs.

Target Gene hoxb1b

Upstream Gene fgf8

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 9

Location of Interaction in evidence presumptive brain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction Expressions of the neuroectodermal genes hoxa-1 was expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.

Target Gene hoxb1b

Upstream Gene zfhx1

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 10.33

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Naomasa Miki

Journal Developmental Biology

Year 2000

Vol 228

Start Page 29

Evidence for Interaction	Expressions of the neuroectodermal genes hoxa-1 was expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.
Target Gene	hoxb1b
Upstream Gene	zfhx1
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	neuroectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Naomasa Miki
Journal	Developmental Biology
Year	2000
Vol	228
Start Page	29

Evidence for Interaction	The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested Fgf17b mRNAs.
Target Gene	hoxb1b
Upstream Gene	fgf17b
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	9
Location of Interaction in evidence	ectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130

Evidence for Interaction	The expression of hoxb1b expanded toward the animal pole in all the fgf17b injected embryos.
Target Gene	hoxb1b
Upstream Gene	fgf17b
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	9
Location of Interaction in evidence	neuroectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130