Expression Table

Gene Name: hoxa9a
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hoxa9a

Stage

Hours

ZFIN expression range

margin

neural plate

paraxial mesoderm

segmental plate

tail bud

central nervous system

notochord

somite

neural tube

spinal cord

whole organism

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction the expression domains of hoxa9a, which share an anterior expression limit with gata1, are significantly reduced in kggtv205 mutants as early as the 5-somite stage.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence paraxial mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction The expression domains of hoxa9b are significantly reduced in kgg tv205 mutants as early as the 3-somite stage.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence paraxial mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction the expression domains of hoxa9a, which share an anterior expression limit with gata1, are significantly reduced in kggtv205 mutants as early as the 5-somite stage.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction The expression domains of hoxa9b are significantly reduced in kgg tv205 mutants as early as the 3-somite stage.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction the expression domains of hoxa9a, which share an anterior expression limit with gata1, are significantly reduced in kggtv205 mutants as early as the 5-somite stage.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction The expression domains of hoxa9b are significantly reduced in kgg tv205 mutants as early as the 3-somite stage.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Hoxa9a display altered expression patterns in kgg tv205 embryos.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence CNS

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Hoxa9a display altered expression patterns in kgg tv205 embryos.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence spinal cord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Hoxa9a display altered expression patterns in kgg tv205 embryos.

Target Gene hoxa9a

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction	the expression domains of hoxa9a, which share an anterior expression limit with gata1, are significantly reduced in kggtv205 mutants as early as the 5-somite stage.
Target Gene	hoxa9a
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	paraxial mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18

Evidence for Interaction	The expression domains of hoxa9b are significantly reduced in kgg tv205 mutants as early as the 3-somite stage.
Target Gene	hoxa9a
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	paraxial mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18

Evidence for Interaction	Hoxa9a display altered expression patterns in kgg tv205 embryos.
Target Gene	hoxa9a
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	CNS
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18