Expression Table

Gene Name: hoxa1a
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hoxa1a	Stage	Hours	ZFIN expression range	brain	forebrain	hindbrain	midbrain	pharyngeal arch	tegmentum	whole organism	unspecified
Zygote	1-cell	0
Cleavage	2-cell	0.75
	4-cell	1.0
	8-cell	1.25
	16-cell	1.5
	32-cell	1.75
	64-cell	2.0
Blastula	124-cell	2.25
	256-cell	2.5
	512-cell	2.75
	1k-cell	3.0
	High	3.33
	Oblong	3.66
	Sphere	4.0
	Dome	4.33
	30% epiboly	4.66
Gastrula	50%-epiboly	5.25								X
	Germ-ring	5.66								X
	Shield	6.0								X
	75% epiboly stage	8.0								X
	90% epiboly	9.0	I							X	I
	bud stage	10.0	I							X	I
Segmentation	1-4 somites	10.33	I							X	I
	5-9 somites	11.66	I							X	I
	10-13 somites	14.0	I							X	I
	14-19 somites	16.0	I							X	I
	20-25 somites	19.0							!		I
	26+ somites	22.0							!		I
Pharyngula	Prim-5	24.0
	Prim-15	30.0
	Prim-25	36.0
	High-pec	42.0
Hatching	Long-pec	48.0
Hatching	Pec-fin	60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Both the presumptive hindbrain and spinal cord marker hoxa1 and the presumptive spinal cord marker vHNF1 are also strongly reduced or lost.

Target Gene hoxa1a

Upstream Gene fzd8c

Interaction Type enhance

Altered model dominant negative

Time of Interaction in evidence 8

Location of Interaction in evidence presumptive brain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Makoto Furutani-Seiki

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 477

Evidence for Interaction The presumptive hindbrain and spinal cord marker hoxa1 is strongly reduced in fzd8c dominant negative embryos.

Target Gene hoxa1a

Upstream Gene fzd8c

Interaction Type enhance

Altered model dominant negative

Time of Interaction in evidence 8

Location of Interaction in evidence presumptive brain

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Makoto Furutani-Seiki

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 477

Evidence for Interaction	Both the presumptive hindbrain and spinal cord marker hoxa1 and the presumptive spinal cord marker vHNF1 are also strongly reduced or lost.
Target Gene	hoxa1a
Upstream Gene	fzd8c
Interaction Type	enhance
Altered model	dominant negative
Time of Interaction in evidence	8
Location of Interaction in evidence	presumptive brain
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Makoto Furutani-Seiki
Journal	Mechanisms of Development
Year	2003
Vol	120
Start Page	477

Evidence for Interaction	The presumptive hindbrain and spinal cord marker hoxa1 is strongly reduced in fzd8c dominant negative embryos.
Target Gene	hoxa1a
Upstream Gene	fzd8c
Interaction Type	enhance
Altered model	dominant negative
Time of Interaction in evidence	8
Location of Interaction in evidence	presumptive brain
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Makoto Furutani-Seiki
Journal	Mechanisms of Development
Year	2003
Vol	120
Start Page	477