Expression Table

Gene Name: hnf1b (HNF1 homeobox b, also known as tcf2, vhnf1, chunp6877, transcription factor 2, mst)
Description: transcription factor activity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hnf1b

Stage

Hours

ZFIN expression range

presumptive brain

presumptive spinal cord

neural plate

hindbrain neural plate

spinal cord neural plate

presumptive rhombomere 5

presumptive rhombomere 6

presumptive rhombomere 7

neural rod

pancreas primordium

pronephros

gut

pancreatic B cell

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction in alk8 (a type I TGFbeta receptor) MO-injected embryos, vhnf1 expression in the endoderm is significantly reduced.

Target Gene hnf1b

Upstream Gene alk8

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 4.66

Location of Interaction in evidence ventral mesoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Lin, S.

Journal Dev Biol

Year 2007

Vol 303

Start Page 561

Evidence for Interaction vhnf1 acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish.

Target Gene hnf1b

Upstream Gene alk8

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 4.66

Location of Interaction in evidence ventral mesoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Lin, S.

Journal Dev Biol

Year 2007

Vol 303

Start Page 561

Evidence for Interaction The presumptive spinal cord marker hnf1b is lost in the dominant negative RNA of fzd8c injected embryo.

Target Gene hnf1b

Upstream Gene fzd8c

Interaction Type enhance

Altered model dominant negative

Time of Interaction in evidence 8

Location of Interaction in evidence presumptive brain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Makoto Furutani-Seiki

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 477

Evidence for Interaction Both the presumptive hindbrain and spinal cord marker hoxa1 and the presumptive spinal cord marker vHNF1 are also strongly reduced or lost.

Target Gene hnf1b

Upstream Gene fzd8c

Interaction Type enhance

Altered model dominant negative

Time of Interaction in evidence 8

Location of Interaction in evidence presumptive brain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Makoto Furutani-Seiki

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 477

Evidence for Interaction hnf1b acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish.

Target Gene hnf1b

Upstream Gene alk8

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 24

Location of Interaction in evidence gut

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Lin, S.

Journal Dev Biol

Year 2007

Vol 303

Start Page 561

Evidence for Interaction Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development. Hnf1b is expressed in the posterior hindbrain up to the r5-r6 boundary (arrowheads) and is shifted anteriorly in Cyp26-depleted embryos.

Target Gene hnf1b

Upstream Gene cyp26c1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence hindbrain

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Moens, C.B.

Journal Development

Year 2007

Vol 134(1)

Start Page 177

Evidence for Interaction hnf1b acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish.

Target Gene hnf1b

Upstream Gene alk8

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 24

Location of Interaction in evidence pronephros

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Lin, S.

Journal Dev Biol

Year 2007

Vol 303

Start Page 561

Evidence for Interaction	in alk8 (a type I TGFbeta receptor) MO-injected embryos, vhnf1 expression in the endoderm is significantly reduced.
Target Gene	hnf1b
Upstream Gene	alk8
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	4.66
Location of Interaction in evidence	ventral mesoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Lin, S.
Journal	Dev Biol
Year	2007
Vol	303
Start Page	561

Evidence for Interaction	vhnf1 acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish.
Target Gene	hnf1b
Upstream Gene	alk8
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	4.66
Location of Interaction in evidence	ventral mesoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Lin, S.
Journal	Dev Biol
Year	2007
Vol	303
Start Page	561

Evidence for Interaction	The presumptive spinal cord marker hnf1b is lost in the dominant negative RNA of fzd8c injected embryo.
Target Gene	hnf1b
Upstream Gene	fzd8c
Interaction Type	enhance
Altered model	dominant negative
Time of Interaction in evidence	8
Location of Interaction in evidence	presumptive brain
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Makoto Furutani-Seiki
Journal	Mechanisms of Development
Year	2003
Vol	120
Start Page	477

Evidence for Interaction	Both the presumptive hindbrain and spinal cord marker hoxa1 and the presumptive spinal cord marker vHNF1 are also strongly reduced or lost.
Target Gene	hnf1b
Upstream Gene	fzd8c
Interaction Type	enhance
Altered model	dominant negative
Time of Interaction in evidence	8
Location of Interaction in evidence	presumptive brain
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Makoto Furutani-Seiki
Journal	Mechanisms of Development
Year	2003
Vol	120
Start Page	477

Evidence for Interaction	hnf1b acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish.
Target Gene	hnf1b
Upstream Gene	alk8
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	24
Location of Interaction in evidence	gut
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Lin, S.
Journal	Dev Biol
Year	2007
Vol	303
Start Page	561

Evidence for Interaction	Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development. Hnf1b is expressed in the posterior hindbrain up to the r5-r6 boundary (arrowheads) and is shifted anteriorly in Cyp26-depleted embryos.
Target Gene	hnf1b
Upstream Gene	cyp26c1
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	hindbrain
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Moens, C.B.
Journal	Development
Year	2007
Vol	134(1)
Start Page	177