Expression Table

Gene Name: hlx
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hlx1

Stage

Hours

ZFIN expression range

endoderm

somite

hypaxial myotome region

cardiovascular system

eye

basal plate midbrain

gut

pharynx

pharyngeal arch

intestinal bulb

dorsal thalamus

pretectum

ventral thalamus

whole organism

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction The expression of hlx1 is reduced in oep mutant embryos at the tail bud stage.

Target Gene hlx1

Upstream Gene oep

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10

Location of Interaction in evidence mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Wolfgang Driever

Journal Development

Year 1997

Vol 124

Start Page 327

Evidence for Interaction Injection of shh RNA eliminates several hlx-1 expression domains in the midbrain.

Target Gene hlx1

Upstream Gene shha

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 30

Location of Interaction in evidence midbrain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Thomas Gerster

Journal Development

Year 1996

Vol 122

Start Page 1769

Evidence for Interaction Injection of shh RNA eliminates several hlx-1 expression domains in the midbrain. Residual hlx-1 midbrain expression is shifted dorsally like the expression domains in the hindbrain.

Target Gene hlx1

Upstream Gene shha

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 30

Location of Interaction in evidence midbrain

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Thomas Gerster

Journal Development

Year 1996

Vol 122

Start Page 1769

Evidence for Interaction	The expression of hlx1 is reduced in oep mutant embryos at the tail bud stage.
Target Gene	hlx1
Upstream Gene	oep
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10
Location of Interaction in evidence	mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Wolfgang Driever
Journal	Development
Year	1997
Vol	124
Start Page	327

Evidence for Interaction	Injection of shh RNA eliminates several hlx-1 expression domains in the midbrain.
Target Gene	hlx1
Upstream Gene	shha
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	30
Location of Interaction in evidence	midbrain
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Thomas Gerster
Journal	Development
Year	1996
Vol	122
Start Page	1769