Expression Table

Gene Name: hhex
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hhex

Stage

Hours

ZFIN expression range

YSL

lateral mesoderm

lateral plate mesoderm

pharyngeal endoderm

dorsal aorta

liver primordium

pancreatic bud

liver

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction hhex expression is initially absent in tbx16, appears at 5s-7s.

Target Gene hhex

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction Hhex expression is initially absent in spt(tbx16) mutain at 5s-7s.

Target Gene hhex

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence hematopoietic system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction In scl(tal1) morphants, hhex expression initiated normally and persisted until 6 somites. After this time, however, levels of expression became progressively reduced, and at 10 somites were lost entirely.

Target Gene hhex

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 14

Location of Interaction in evidence blood precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction hhex expression is initially absent in tbx16, appears at 5s-7s.

Target Gene hhex

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction In tal1 morphants, hhex expression initiated normally and persisted until 6 somites (12 hpf). After this time, however, levels of expression became progressively reduced, and at 10 somites (14 hpf) were lost entirely.

Target Gene hhex

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 14

Location of Interaction in evidence blood

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction Hhex expression is absent in spt(tbx16) mutain at 12s.

Target Gene hhex

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence hematopoietic system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction	hhex expression is initially absent in tbx16, appears at 5s-7s.
Target Gene	hhex
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	blood
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	Hhex expression is initially absent in spt(tbx16) mutain at 5s-7s.
Target Gene	hhex
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	11.66
Location of Interaction in evidence	hematopoietic system
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	In scl(tal1) morphants, hhex expression initiated normally and persisted until 6 somites. After this time, however, levels of expression became progressively reduced, and at 10 somites were lost entirely.
Target Gene	hhex
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	14
Location of Interaction in evidence	blood precursors
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	In tal1 morphants, hhex expression initiated normally and persisted until 6 somites (12 hpf). After this time, however, levels of expression became progressively reduced, and at 10 somites (14 hpf) were lost entirely.
Target Gene	hhex
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	14
Location of Interaction in evidence	blood
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	Hhex expression is absent in spt(tbx16) mutain at 12s.
Target Gene	hhex
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	hematopoietic system
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251