Expression Table

Gene Name: hey2
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hey2

Stage

Hours

ZFIN expression range

neural plate

mesoderm

ventral mesoderm

tail bud

somite

cranial ganglia

neural rod

olfactory placode

brain

diencephalon

midbrain

pharyngeal arch

intermediate cell mass of mesoderm

artery

blood

ventricular zone

optic tectum

peripheral olfactory organ

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Real-time RT-PCR analyses showing the increased expression of cmlc2 in grlm145 mutants at 48 hpf.

Target Gene cmlc1

Upstream Gene hey2

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 48

Location of Interaction in evidence heart

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Tao P.Zhong

Journal PNAS

Year 2007

Vol 104

Start Page 14008

Evidence for Interaction Real-time RT-PCR analyses showing the increased expression of vmhc in grlm145 mutants at 48 hpf.

Target Gene vmhc

Upstream Gene hey2

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 48

Location of Interaction in evidence heart

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Tao P.Zhong

Journal PNAS

Year 2007

Vol 104

Start Page 14008

Evidence for Interaction	Real-time RT-PCR analyses showing the increased expression of cmlc2 in grlm145 mutants at 48 hpf.
Target Gene	cmlc1
Upstream Gene	hey2
Interaction Type	repress
Altered model	mutant embryos
Time of Interaction in evidence	48
Location of Interaction in evidence	heart
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Tao P.Zhong
Journal	PNAS
Year	2007
Vol	104
Start Page	14008