Expression Table

Gene Name: her5
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

her5

Stage

Hours

ZFIN expression range

presumptive endoderm

margin

hypoblast

presumptive midbrain hindbrain boundary

neural plate

midbrain hindbrain boundary neural plate

midbrain hindbrain boundary neural keel

segmental plate

midbrain hindbrain boundary neural rod

midbrain

midbrain hindbrain boundary

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Her5 expression at the MHB is reduced in spg mutant embryos around 70% of epiboly.

Target Gene her5

Upstream Gene pou5f1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence mesendoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2002

Vol 129

Start Page 917

Evidence for Interaction Her5 expression at the MHB is reduced in spg mutant embryos around 70% of epiboly.

Target Gene her5

Upstream Gene pou5f1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2002

Vol 129

Start Page 917

Evidence for Interaction Her5 expression at the MHB is reduced in spg mutant embryos at the end of gastrulation.

Target Gene her5

Upstream Gene pou5f1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2002

Vol 129

Start Page 917

Evidence for Interaction In noi -/ - (pax2a) mutants, her5 expression is initiated normally, but maintenance of expression becomes abnormal from the 6-somite stage onwards.

Target Gene her5

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence midbrain

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction In noi -/ - mutants expression is initiated normally, but maintenance of expression becomes abnormal from the 6-somite stage onwards.

Target Gene her5

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence midbrain

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction In noi -/ - (pax2a) mutants, her5 expression is initiated normally, but maintenance of expression becomes abnormal from the 6-somite stage onwards.

Target Gene her5

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction In noi -/ - mutants expression is initiated normally, but maintenance of expression becomes abnormal from the 6-somite stage onwards.

Target Gene her5

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction In noi -/ - (pax2a) mutants, her5 expression is completely absent at 26 hours.

Target Gene her5

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 26

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction Eng1 and her5 expression is completely absent at 26 hours.

Target Gene her5

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 26

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction 36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos.

Target Gene her5

Upstream Gene oep

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.25

Location of Interaction in evidence margin

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Schier, A. F.

Journal Dev Biol

Year 2007

Vol 304

Start Page 525

Evidence for Interaction	Her5 expression at the MHB is reduced in spg mutant embryos around 70% of epiboly.
Target Gene	her5
Upstream Gene	pou5f1
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	8
Location of Interaction in evidence	mesendoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	2002
Vol	129
Start Page	917

Evidence for Interaction	In noi -/ - (pax2a) mutants, her5 expression is initiated normally, but maintenance of expression becomes abnormal from the 6-somite stage onwards.
Target Gene	her5
Upstream Gene	pax2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	11.66
Location of Interaction in evidence	midbrain
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	1998
Vol	125
Start Page	3049

Evidence for Interaction	In noi -/ - mutants expression is initiated normally, but maintenance of expression becomes abnormal from the 6-somite stage onwards.
Target Gene	her5
Upstream Gene	pax2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	11.66
Location of Interaction in evidence	midbrain
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	1998
Vol	125
Start Page	3049

Evidence for Interaction	Eng1 and her5 expression is completely absent at 26 hours.
Target Gene	her5
Upstream Gene	pax2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	26
Location of Interaction in evidence	MHB
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	1998
Vol	125
Start Page	3049

Evidence for Interaction	36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos.
Target Gene	her5
Upstream Gene	oep
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.25
Location of Interaction in evidence	margin
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Schier, A. F.
Journal	Dev Biol
Year	2007
Vol	304
Start Page	525