Expression Table

Gene Name: hbbe3
Description: oxygen transporter activity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hbbe3	Stage	Hours	ZFIN expression range	presumptive blood	intermediate cell mass of mesoderm	blood	unspecified
Zygote	1-cell	0
Cleavage	2-cell	0.75
	4-cell	1.0
	8-cell	1.25
	16-cell	1.5
	32-cell	1.75
	64-cell	2.0
Blastula	124-cell	2.25
	256-cell	2.5
	512-cell	2.75
	1k-cell	3.0
	High	3.33
	Oblong	3.66
	Sphere	4.0
	Dome	4.33
	30% epiboly	4.66
Gastrula	50%-epiboly	5.25
	Germ-ring	5.66
	Shield	6.0
	75% epiboly stage	8.0
	90% epiboly	9.0
	bud stage	10.0
Segmentation	1-4 somites	10.33
	5-9 somites	11.66
	10-13 somites	14.0
	14-19 somites	16.0
	20-25 somites	19.0
	26+ somites	22.0
Pharyngula	Prim-5	24.0
	Prim-15	30.0					I
	Prim-25	36.0	I			I	I
	High-pec	42.0	I			I	I
Hatching	Long-pec	48.0
Hatching	Pec-fin	60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction kggtv205 mutants have a severe reduction in the number of haemoglobin-expressing erythroid cells compared with wild-type siblings.

Target Gene hbbe3

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction By 24 h post-fertilization, kgg tv205 mutants have a severe reduction in the number of haemoglobin-expressing erythroid cells.

Target Gene hbbe3

Upstream Gene cdx4

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction	kggtv205 mutants have a severe reduction in the number of haemoglobin-expressing erythroid cells compared with wild-type siblings.
Target Gene	hbbe3
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	blood
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18

Evidence for Interaction	By 24 h post-fertilization, kgg tv205 mutants have a severe reduction in the number of haemoglobin-expressing erythroid cells.
Target Gene	hbbe3
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	blood
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18