Expression Table

Gene Name: hbbe1
Description: oxygen transporter activity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hbbe1	Stage	Hours	ZFIN expression range	intermediate cell mass of mesoderm	blood	ventral wall of dorsal aorta	whole organism	unspecified
Zygote	1-cell	0
Cleavage	2-cell	0.75
	4-cell	1.0
	8-cell	1.25
	16-cell	1.5
	32-cell	1.75
	64-cell	2.0					X
Blastula	124-cell	2.25					X
	256-cell	2.5					X
	512-cell	2.75					X
	1k-cell	3.0					X
	High	3.33					X
	Oblong	3.66					X
	Sphere	4.0					X
	Dome	4.33					X
	30% epiboly	4.66					X
Gastrula	50%-epiboly	5.25					X
	Germ-ring	5.66					X
	Shield	6.0
	75% epiboly stage	8.0
	90% epiboly	9.0
	bud stage	10.0
Segmentation	1-4 somites	10.33
	5-9 somites	11.66
	10-13 somites	14.0
	14-19 somites	16.0
	20-25 somites	19.0
	26+ somites	22.0
Pharyngula	Prim-5	24.0
	Prim-15	30.0
	Prim-25	36.0	I		I
	High-pec	42.0	I		I
Hatching	Long-pec	48.0	I		I
Hatching	Pec-fin	60.0	I		I

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Circulating embryonic RBCs are absent in tbx16 embryos, embryonic hbbe1 expression is also lost at 24 hpf.

Target Gene hbbe1

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction Expression of beta embryonic globin 1 in the primitive erythroid lineage was lost entirely in scl spliceMO-injected embryos.

Target Gene hbbe1

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 24

Location of Interaction in evidence primitive erythroid lineage

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 3502

Evidence for Interaction Expression of hebb1 was lost entirely in the tal1 MO embryos.

Target Gene hbbe1

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 24

Location of Interaction in evidence primitive erythroid lineage

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction Embryonic be1globin expression is lost at 24 hpf in spt embryos.

Target Gene hbbe1

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence RBC markers

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction	Circulating embryonic RBCs are absent in tbx16 embryos, embryonic hbbe1 expression is also lost at 24 hpf.
Target Gene	hbbe1
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	blood
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	Expression of beta embryonic globin 1 in the primitive erythroid lineage was lost entirely in scl spliceMO-injected embryos.
Target Gene	hbbe1
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	24
Location of Interaction in evidence	primitive erythroid lineage
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	3502

Evidence for Interaction	Expression of hebb1 was lost entirely in the tal1 MO embryos.
Target Gene	hbbe1
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	24
Location of Interaction in evidence	primitive erythroid lineage
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	Embryonic be1globin expression is lost at 24 hpf in spt embryos.
Target Gene	hbbe1
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	RBC markers
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251