Expression Table

Gene Name: hbae1
Description: oxygen transporter activity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

hbae1

Stage

Hours

ZFIN expression range

blastomere

presumptive blood

ventral mesoderm

lateral plate mesoderm

intermediate cell mass of mesoderm

blood

nucleate erythrocyte

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

X-I

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Circulating embryonic RBCs are absent in tbx16 mutant embryos, embryonic ahbae1 expression is also lost at 24 hpf.

Target Gene hbae1

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction Embryonic ae1globin expression is lost at 24 hpf in spt embryos.

Target Gene hbae1

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 24

Location of Interaction in evidence RBC markers

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction	Circulating embryonic RBCs are absent in tbx16 mutant embryos, embryonic ahbae1 expression is also lost at 24 hpf.
Target Gene	hbae1
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	blood
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	Embryonic ae1globin expression is lost at 24 hpf in spt embryos.
Target Gene	hbae1
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	24
Location of Interaction in evidence	RBC markers
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251