Expression Table

Gene Name: gsc
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007
gscStageHoursZFIN expression rangeblastomeremarginal blastomereYSLpresumptive mesodermpresumptive shieldDELblastodermmarginaxisgerm ringhypoblastshieldanterior axial hypoblastaxial chorda mesodermneural plateaxial mesodermmesodermprechordal plateforebrain neural keelneural keelpolsterheadpresumptive diencephalonpresumptive telencephalondiencephalonpharyngeal arch 3-7 skeletontelencephalonpharyngeal archotic vesiclepectoral fin budpectoral finMeckel's cartilagedorsal hyoid archmandibular archpalatoquadrate archpharyngeal arch 2 skeletonpharyngeal arch cartilagesymplecticventral hyoid archwhole organismunspecified
Zygote1-cell0                                        ? 
Cleavage2-cell0.75                                        ? 
4-cell1.0                                        ? 
8-cell1.25                                        ? 
16-cell1.5                                        ? 
32-cell1.75                                        ? 
64-cell2.0                                        ? 
Blastula124-cell2.25                                        ? 
256-cell2.5                                        ?I
512-cell2.75                                        ?I
1k-cell3.0                                         I
High3.33                                          
Oblong3.66  I                                      I
Sphere4.0        !                               ? 
Dome4.33        !                                 
30% epiboly4.66  !                                       
Gastrula50%-epiboly5.25       I     ! ! !                        
Germ-ring5.66    I  II    ! ! !                        
Shield6.0   X   I     ! ! !!                       
75% epiboly stage8.0        X                                 
90% epiboly9.0                    !                     
bud stage10.0                                          
Segmentation1-4 somites10.33                                         I
5-9 somites11.66                   !                    ?I
10-13 somites14.0                   !                    ?I
14-19 somites16.0I                                       ?I
20-25 somites19.0                              !         ? 
26+ somites22.0                              !         ?I
PharyngulaPrim-524.0                                         I
Prim-1530.0                                          
Prim-2536.0                                          
High-pec42.0                                  I I     
HatchingLong-pec48.0                                          
Pec-fin60.0                      I         I I I     


Literature Evidence



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of gsca at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn the boz mutant embryos, the expression of gsc and din was detected at slightly reduced levels at the sphere stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionExpression of gsc at the sphere stage was inhibited by the injection of axin RNA
Target Genegsc
Upstream Geneaxin1
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionExpression of gsc and din at the sphere stage was also inhibited by the injection of axin and axil RNA.
Target Genegsc
Upstream Geneaxin1
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionExpression of gsc at the sphere stage was inhibited by the injection of axin RNA
Target Genegsc
Upstream Geneaxin2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionExpression of gsc and din at the sphere stage was also inhibited by the injection of axin and axil RNA
Target Genegsc
Upstream Geneaxin2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn contrast, injection of b-catenin RNA elicited the ectopic expression of boz/dharma, sqt, gsc, and din at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of gsca at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma did not affect the expression of gsc and din at the sphere stage, but elicited a strong increase in the expression of these genes in the blastoderm margin at the shield stage. In the boz mutant embryos, the expression of g
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma did not affect the expression of gsc and din at the sphere stage, but elicited a strong increase in the expression of these genes in the blastoderm margin at the shield stage. In the boz mutant embryos, the expression of g
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn the boz mutant embryos, the expression of gsc and din was detected at slightly reduced levels at the sphere stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expresse
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expresse
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionThe expression of ved was downregulated by the overexpression of Dha/Boz and gsc. Genotyping of the boz mutant embryos was performed by PCR after the photographs were taken. Animal pole view, dorsal is to the right.
Target Geneved
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidenceEVL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionThe expression of ved was downregulated by the overexpression of gsc.
Target Geneved
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidenceEVL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionExpression of gsc at the sphere stage was inhibited by the injection of axin RNA
Target Genegsc
Upstream Geneaxin1
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionExpression of gsc and din at the sphere stage was also inhibited by the injection of axin and axil RNA.
Target Genegsc
Upstream Geneaxin1
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionExpression of gsc at the sphere stage was inhibited by the injection of axin RNA
Target Genegsc
Upstream Geneaxin2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionExpression of gsc and din at the sphere stage was also inhibited by the injection of axin and axil RNA
Target Genegsc
Upstream Geneaxin2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of gsca at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn contrast, injection of b-catenin RNA elicited the ectopic expression of boz/dharma, sqt, gsc, and din at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma did not affect the expression of gsc and din at the sphere stage, but elicited a strong increase in the expression of these genes in the blastoderm margin at the shield stage. In the boz mutant embryos, the expression of g
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma did not affect the expression of gsc and din at the sphere stage, but elicited a strong increase in the expression of these genes in the blastoderm margin at the shield stage. In the boz mutant embryos, the expression of g
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn the boz mutant embryos, the expression of gsc and din was detected at slightly reduced levels at the sphere stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactiongsc-injected embryos displayed activation of pitx2a and pitx2c in both marginal and animal pole cells.
Target Genepitx2a
Upstream Genegsc
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year2000
Vol127
Start Page1081



Evidence for Interactiongsc-injected embryos displayed activation of pitx2a in both marginal and animal pole cells
Target Genepitx2a
Upstream Genegsc
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year2000
Vol127
Start Page1081



Evidence for Interactiongsc-injected embryos displayed activation of pitx2a and pitx2c in both marginal and animal pole cells
Target Genepitx2c
Upstream Genegsc
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year2000
Vol127
Start Page1081



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expresse
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expresse
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionEmbryos injected with 100 pg of ved RNA or 100 pg of En-ved RNA displayed a strongly ventralized phenotype at 24 h.p.f. and reduced expression of dha/boz, gsc, and din.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionEmbryos injected with ved RNA reduced expression of gsc at 24 h.p.f.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionEmbryos injected with 100 pg of ved RNA or 100 pg of En-ved RNA displayed a strongly ventralized phenotype at 24 h.p.f. and reduced expression of dha/boz, gsc, and din.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionEmbryos injected with ved RNA reduced expression of gsc at 24 h.p.f.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionExpression of gsc in prechordal plateprogenitors is weakly reduced in bon, strongly reduced in Mzsur and absent in MZsur;bon double mutant embryos.
Target Genegsc
Upstream Genebon
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2003
Vol130
Start Page5589



Evidence for InteractionExpression of gsc in prechordal plate progenitors is weakly reduced in bon, strongly reduced in Mzsur and absent in MZsur;bon mutant embryos.
Target Genegsc
Upstream Genebon
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2003
Vol130
Start Page5589



Evidence for Interactionoverexpression of either En-boz or boz mRNA induced ectopic chd and gsc expression in 50% epiboly stage wild-type embryos.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year2003
Vol130
Start Page3639



Evidence for Interactionoverexpression of either En-boz or boz mRNA induced ectopic chd and gsc expression in 50% epiboly stage wild-type embryos. Thus, the En-Boz fusion protein has activities similar to wild-type Boz: overexpression of either Enboz or boz mRNA can rescue boz m
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year2003
Vol130
Start Page3639



Evidence for InteractionExpression of gsc in prechordal plateprogenitors is weakly reduced in bon, strongly reduced in foxh1 and absent in foxh1;bon mutant embryos.
Target Genegsc
Upstream Genefoxh1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2003
Vol130
Start Page5589



Evidence for InteractionExpression of gsc in prechordal plate progenitors is weakly reduced in bon, strongly reduced in Mzsur and absent in MZsur;bon mutant embryos.
Target Genegsc
Upstream Genefoxh1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2003
Vol130
Start Page5589



Evidence for InteractionConcomitantly, the axial mesoderm domain, expressing gsc, is expanded at 50% epiboly.
Target Genegsc
Upstream Genefzd8c
Interaction Typerepress
Altered modeldominant negative
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorMakoto Furutani-Seiki
JournalMechanisms of Development
Year2003
Vol120
Start Page477



Evidence for InteractionOverexpression of cyclops and squint at different doses leads to the induction of floating head at low doses and the induction of both goosecoid (Gsc) and floating head (Flh) at higher doses
Target GeneGsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionDorsal views at 50-60% epiboly.flh; gsc; twhh.Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain , but not of gsc or twhh. Injections of higher doses of cyc+ R
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionOverexpression of cyclops and squint at different doses leads to the induction of floating head at low doses and the induction of both goosecoid (Gsc) and floating head (Flh) at higher doses
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionIn embryos injected with pitx2a RNA, the number of gsc expressing cells on the dorsal side was reduced
Target Genegsc
Upstream Genepitx2a
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencedorsal side
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year2000
Vol127
Start Page1081



Evidence for InteractionDuring gastrulation, expression of goosecoid (gsc) becomes reduced in sox 32 overexpressed embryos
Target Genegsc
Upstream Genesox32
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHiroyuki Takeda
JournalMechanisms of Development
Year2001
Vol107
Start Page25



Evidence for Interactionduring gastrulation, expression of goosecoid (gsc) becomes reduced in overexpressed embryos.
Target Genegsc
Upstream Genesox32
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHiroyuki Takeda
JournalMechanisms of Development
Year2001
Vol107
Start Page25



Evidence for InteractionDuring gastrulation, expression of goosecoid (gsc) becomes reduced in sox 32 overexpressed embryos
Target Genegsc
Upstream Genesox32
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHiroyuki Takeda
JournalMechanisms of Development
Year2001
Vol107
Start Page25



Evidence for Interactiongsc expression is down-regulated in ved-injected embryos (67%; n 82) and is ectopically activated in the cells adjacent to the anterior shield, at the level of the blastoderm margin, in ved MO-injected embryos (82%; n= 65).
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorFranco Cotelli
JournalDevelopmental Dynamics
Year2004
Vol230
Start Page494



Evidence for Interactiongsc expression is down-regulated in ved-injected embryos.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorFranco Cotelli
JournalDevelopmental Dynamics
Year2004
Vol230
Start Page494



Evidence for Interactiongsc expression is down-regulated in ved-injected embryos (67%; n 82) and is ectopically activated in the cells adjacent to the anterior shield, at the level of the blastoderm margin, in ved MO-injected embryos (82%; n= 65).
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modelMASO
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorFranco Cotelli
JournalDevelopmental Dynamics
Year2004
Vol230
Start Page494



Evidence for Interactiongsc expression is down-regulated in ved-injected embryos.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modelMASO
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorFranco Cotelli
JournalDevelopmental Dynamics
Year2004
Vol230
Start Page494



Evidence for InteractionExpression of organizer genes, gsc, floating head (flh), chordin (din), and cyclops (cyc) were strongly suppressed by vega2-HA mRNA injection.
Target Genegsc
Upstream Genevent
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for InteractionExpression of organizer genes, gsc was strongly suppressed by vega2-HA mRNA injection
Target Genegsc
Upstream Genevent
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactiongsc expressed in axial mesoderm is expended in high dose (250 pg) fzb1 mRNA injected embryos at 50% epiboly.
Target Genegsc
Upstream Genewnt8
Interaction Typerepress
Altered modeldominant negative
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorMakoto Furutani-Seiki
JournalMechanisms of Development
Year2003
Vol120
Start Page477



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for Interactionoverexpression of vent also reduced the expanded domains of gsc and chd in Df st7 mutants and reduced the dorsal domains of gsc and chd expression in wild-type embryos.
Target Genegsc
Upstream Genevent
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for Interactionoverexpression of vent reduced the dorsal domains of gsc.
Target Genegsc
Upstream Genevent
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for InteractionExpression of organizer genes, gsc was strongly suppressed by vega2-HA mRNA injection
Target Genegsc
Upstream Genevent
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactionoverexpression of vent also reduced the expanded domains of gsc and chd in Df st7 mutants and reduced the dorsal domains of gsc and chd expression in wild-type embryos.
Target Genegsc
Upstream Genevox
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactionoverexpression of vox reduced the expansion of gsc and chd in Df st7 mutants, and also reduced the domains of expression in wild-type embryos.
Target Genegsc
Upstream Genevox
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for Interactionoverexpression of vox reduced the expansion of gsc in Df st7 mutants.
Target Genegsc
Upstream Genevox
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for Interactionoverexpression of vent also reduced the expanded domains of gsc and chd in Df st7 mutants and reduced the dorsal domains of gsc and chd expression in wild-type embryos.
Target Genegsc
Upstream Genevent
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for Interactionoverexpression of vent reduced the dorsal domains of gsc.
Target Genegsc
Upstream Genevent
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for Interactionoverexpression of vent also reduced the expanded domains of gsc and chd in Df st7 mutants and reduced the dorsal domains of gsc and chd expression in wild-type embryos.
Target Genegsc
Upstream Genevox
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactionoverexpression of vox reduced the expansion of gsc and chd in Df st7 mutants, and also reduced the domains of expression in wild-type embryos.
Target Genegsc
Upstream Genevox
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for Interactionoverexpression of vox reduced the expansion of gsc in Df st7 mutants.
Target Genegsc
Upstream Genevox
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorWilliam S. Talbot
JournalDevelopment
Year2001
Vol128
Start Page2407



Evidence for Interactiongsc-injected embryos displayed activation of pitx2a and pitx2c in both marginal and animal pole cells
Target Genepitx2c
Upstream Genegsc
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence5.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year2000
Vol127
Start Page1081



Evidence for InteractionEexpression of gsc and din at the shield stage increased in the embryos injected with axin, axil, or axin and gsk3 a RNA
Target Genegsc
Upstream Geneaxin1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionEexpression of gsc and din at the shield stage increased in the embryos injected with axin, axil, or axin and gsk3 a RNA
Target Genegsc
Upstream Geneaxin2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionLithium chloride treatment (0.3M LiCl at the 64-256 cell stage), which is known to activate b-catenin signaling, resulted in expanded gsc expression.
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeldrug induce overexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of boz/dharma, sqt, gsc, and din at the sphere and shield stages.
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionLithium chloride treatment (0.3M LiCl at the 64-256 cell stage), which is known to activate b-catenin signaling, resulted in the strong inhibition of vega2 expression at the shield stage, whereas gsc expression was expanded.
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeldrug induce overexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn the boz mutant embryos, the expression of gsc was detected at slightly reduced levels at the sphere stage, but was barely detectable at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionEmbryos injected with 5 pg of dha/ boz RNA or 5 pg of En-dharma RNA displayed a strongly dorsalized phenotype at 24 hrs and expanded expression of gsc at the shield stage, compared with wild-type embryos .
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionRadial expression of gsc in the entire margin of embryos following injection of wild-type nieuwkoid/dharma RNA into both blastomeres at the two-cell stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for Interactionoverexpression of boz/dharma did not affect the expression of gsc and din at the sphere stage, but elicited a strong increase in the expression of these genes in the blastoderm margin at the shield stage. In the boz mutant embryos, the expression of gs
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma did not affect the expression of gsc and din at the sphere stage, but elicited a strong increase in the expression of these genes in the blastoderm margin at the shield stage. In the boz mutant embryos, the expression of gs
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionRadial expression of gsc in the entire margin of embryos following injection of wild-type nieuwkoid/dharma RNA into both blastomeres at the two-cell stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for InteractionEmbryos injected with 5 pg of dha/boz RNA or 5 pg of En-dharma RNA displayed a strongly dorsalized phenotype at 24 h.p.f. and expanded expression of gsc wild-type embryos.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionEctopic expression of gsc was observed in bozmutant embryos following overexpression.
Target Genegsc
Upstream Geneeomes
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopment
Year2003
Vol130
Start Page5503



Evidence for InteractionNormally, gsc is expressed in cells that give rise to the prechordal plate whereas chd is expressed more broadly on the dorsal side of the embryo. Ectopic expression of gsc and chd was detected in eomes- and myc-eomes-injected embryos along the margin at
Target Genegsc
Upstream Geneeomes
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopment
Year2003
Vol130
Start Page5503



Evidence for InteractionEctopic expression of gsc (85%, 17/20), chd (100%, 13/13) and flh (100%, 15/15) was observed in bozmutant embryos following overexpression, which showed that nwk/dhm gene function does not appear to be required for eomes to induce expression of organizer-
Target Genegsc
Upstream Geneeomes
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopment
Year2003
Vol130
Start Page5503



Evidence for InteractionInjection of mRNAs encoding the b-catenin target genes boz/dha or gsc strongly inhibited vega2 expression. Boz/dha dependent suppression of vega2 is also indicated by the ectopic expression of vega2 transcripts in dorsal blastomeres of boz mutant embryos.
Target Genevent
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for InteractionIinjection of mRNAs encoding the b-catenin target genes gsc strongly inhibited vega2 expression.
Target Genevent
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactiongsc expression is reduced in embryo injected with the DN-IGF-1R mRNA.
Target Genegsc
Upstream Geneigf1rb
Interaction Typeenhance
Altered modeldominant negative
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorLUCY BYRNES
JournalInt. J. Dev. Biol.
Year2004
Vol48
Start Page1131



Evidence for InteractionReduction in the expression of gsc in embryo injected with the DN-IGF-1R mRNA.
Target Genegsc
Upstream Geneigf1rb
Interaction Typeenhance
Altered modeldominant negative
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorLUCY BYRNES
JournalInt. J. Dev. Biol.
Year2004
Vol48
Start Page1131



Evidence for Interactionoverexpression of sqt (ndr1) elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was ex
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt (ndr1) elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was ex
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of sqt elicited the ectopic expression of gsc and din in the entire blastoderm at the sphere to shield stage, but did not change the expression of boz/dharma. In the sqt mutant embryos, gsc expression was not detected, but din was expressed
Target Genegsc
Upstream Genendr1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionInjections of higher doses of cyc+ (ndr2) RNA result in expansion of the gsc expression domain in the shield.
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionInjections of higher doses of cyc+ (ndr2) RNA result in expansion of the gsc expression domain in the shield.
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionDorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh Injections of higher doses of cyc+ R
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionDorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh Injections of higher doses of cyc+ R
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionInjections of higher doses of cyc+ (ndr2) RNA result in expansion of the gsc expression domain in the shield.
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionEmbryos injected with 100 pg of ved RNA or 100 pg of En-ved RNA displayed a strongly ventralized phenotype at 24 h.p.f. and reduced expression of dha/boz, gsc, and din.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionEmbryos injected with ved RNA reduced expression of gsc.
Target Genegsc
Upstream Geneved
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionExpression analysis of the dorsal markers chd, gsc, floating head (flh) and dharma (bozozok) at shield stage shows that they are expanded ventrally in wnt8 mutants (Fig. 1B,F) (Lekven et al., 2001) (and data not shown) as well as in vent;vox mutants (Fig.
Target Genegsc
Upstream Genewnt8a
Interaction Typerepress
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorArne C. Lekven
JournalDevelopment
Year2004
Vol131
Start Page3991



Evidence for InteractionAt shield stage, expression of the dorsal marker gsc is expanded ventrally in wnt8 mutants.
Target Genegsc
Upstream Genewnt8a
Interaction Typerepress
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorArne C. Lekven
JournalDevelopment
Year2004
Vol131
Start Page3991



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemargin
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn the boz mutant embryos, the expression of gsc was detected at slightly reduced levels at the sphere stage, but was barely detectable at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidencemargin
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionThe expression of ved was down regulated by the overexpression of Dha/Boz and gsc. Genotyping of the boz mutant embryos was performed by PCR after the photographs were taken. Animal pole view, dorsal is to the right.
Target Geneved
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionThe expression of ved was downregulated by the overexpression of gsc.
Target Geneved
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionInjection of mRNAs encoding the b-catenin target genes boz/dha or gsc strongly inhibited vega2 expression. Boz/dha dependent suppression of vega2 is also indicated by the ectopic expression of vega2 transcripts in dorsal blastomeres of boz mutant embryos.
Target Genevent
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for InteractionIinjection of mRNAs encoding the b-catenin target genes gsc strongly inhibited vega2 expression.
Target Genevent
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidenceYSL
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn the boz mutant embryos, the expression of gsc was detected at slightly reduced levels at the sphere stage, but was barely detectable at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for Interactionoverexpression of boz/dharma elicited a strong increase in the expression of gsc in the blastoderm margin at the shield stage.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence6
Location of Interaction in evidenceYSL
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionThe expression of ved was down regulated by the overexpression of Dha/Boz and gsc. Genotyping of the boz mutant embryos was performed by PCR after the photographs were taken. Animal pole view, dorsal is to the right.
Target Geneved
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionThe expression of ved was downregulated by the overexpression of gsc.
Target Geneved
Upstream Genegsc
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2002
Vol118
Start Page125



Evidence for InteractionIn homozygous bozm168 mutant embryos, gsc expression is reduced or absent.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for Interactiongsc expression in the wild-type midgastrula is restricted to the cells of the anteriorly migrating prechordal plate. In homozygous bozm168 mutant embryos, gsc expression is reduced or absent.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the con
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the co
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the con
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the co
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionCompared with wild-type embryos or cycsg1 mutant embryos at 28.5°C, gsc expression is variably reduced in cycsg1 mutant embryos at 22°C.
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionCompared with wild-type embryos or cycsg1 mutant embryos at 28.5°C, gsc expression is variably reduced in cycsg1 mutant embryos at 22°C.
Target Genegsc
Upstream Genendr2
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorKaruna Sampath
JournalDevelopment
Year2003
Vol130
Start Page3331



Evidence for InteractionExpression of goosecoid appeared normal in most of the injected embryos when examined at the shield stage; however, in about 20% of these embryos, expression of goosecoid was drastically reduced or lost at 70% epiboly ( n=36)
Target Genegsc
Upstream Geneog9x
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorThierry Lepage
JournalDevelopment
Year2002
Vol129
Start Page4901



Evidence for InteractionExpression of goosecoid appeared normal in most of the injected embryos when examined at the shield stage; however, in about 20% of these embryos, expression of goosecoid was drastically reduced or lost at 70% epiboly.
Target Genegsc
Upstream Geneog9x
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidencemesendoderm
Evidence for Direct1, 2
Binding Site Sequence 
Location of Site 
Last AuthorThierry Lepage
JournalDevelopment
Year2002
Vol129
Start Page4901



Evidence for InteractionIn homozygous bozm168 mutant embryos, gsc expression is reduced or absent.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidencemesoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for Interactiongsc expression in the wild-type midgastrula is restricted to the cells of the anteriorly migrating prechordal plate. In homozygous bozm168 mutant embryos, gsc expression is reduced or absent.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidencemesoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the con
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence8
Location of Interaction in evidencemesoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the co
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence8
Location of Interaction in evidencemesoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the con
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidencemesoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the co
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidencemesoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn embryos injected with pitx2a or pitx2c RNA, the number of gsc expressing cells on the dorsal side was reduced
Target Genegsc
Upstream Genepitx2a
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidencemesoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year2000
Vol127
Start Page1081



Evidence for InteractionIn homozygous bozm168 mutant embryos, gsc expression is reduced or absent.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidenceneuroectoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for Interactiongsc expression in the wild-type midgastrula is restricted to the cells of the anteriorly migrating prechordal plate. In homozygous bozm168 mutant embryos, gsc expression is reduced or absent.
Target Genegsc
Upstream Genedharma
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence8
Location of Interaction in evidenceneuroectoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorRobert K. Ho
JournalDevelopmental Biology
Year1999
Vol215
Start Page190



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the con
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence8
Location of Interaction in evidenceneuroectoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the co
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence8
Location of Interaction in evidenceneuroectoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the con
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidenceneuroectoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionIn situ hybridization showed that embryos injected with gelsolin mRNA up-regulated chordin and goosecoid transcripts. Conversely, gelsolin MO-injected embryos had lower intensity hybridization signals for chordin and goosecoid transcripts than did the co
Target Genegsc
Upstream Genegsn
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence8
Location of Interaction in evidenceneuroectoderm
Evidence for Direct2, 3
Binding Site Sequence 
Location of Site 
Last AuthorJoram Piatigorsky
JournalPNAS
Year2003
Vol100
Start Page3287



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants. gsc expression at 45% epiboly;; 50% epiboly; after shield formation and at 90% epiboly in wild-type and oep mutant embryos.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidenceectoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidenceectoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for Interactiongsc expression is abolished by injection of 25 pg lft2-MT RNA.
Target Genegsc
Upstream Genelft2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence9
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year1999
Vol126
Start Page3253



Evidence for InteractionExpress gsc in a 90 degree arc at the margin. gsc expression is abolished by injection of 25 pg lft2-MT RNA.
Target Genegsc
Upstream Genelft2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence9
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year1999
Vol126
Start Page3253



Evidence for Interactiongsc expression is abolished by injection of 25 pg lft2-MT RNA.
Target Genegsc
Upstream Genelft2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence9
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year1999
Vol126
Start Page3253



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants. gsc expression at 45% epiboly;; 50% epiboly; after shield formation and at 90% epiboly in wild-type and oep mutant embryos.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemesendoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionAt 90% epiboly, the gsc expression domain is reduced in bon-/- embryos.
Target Genegsc
Upstream Genebon
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemesoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorDidier Y. R. Stainier
JournalDevelopment
Year2003
Vol130
Start Page4989



Evidence for InteractionAt 90% epiboly, the gsc expression domain is reduced in bon-/- embryos, indicating a differentiation defect in the anterior axial mesoderm.
Target Genegsc
Upstream Genebon
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemesoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorDidier Y. R. Stainier
JournalDevelopment
Year2003
Vol130
Start Page4989



Evidence for Interactiongsc expression is abolished by injection of 25 pg lft2-MT RNA.
Target Genegsc
Upstream Genelft2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence9
Location of Interaction in evidencemesoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year1999
Vol126
Start Page3253



Evidence for InteractionExpress gsc in a 90 degree arc at the margin. gsc expression is abolished by injection of 25 pg lft2-MT RNA.
Target Genegsc
Upstream Genelft2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence9
Location of Interaction in evidencemesoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year1999
Vol126
Start Page3253



Evidence for Interactiongsc expression is abolished by injection of 25 pg lft2-MT RNA.
Target Genegsc
Upstream Genelft2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence9
Location of Interaction in evidencemesoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorH. Joseph Yost
JournalDevelopment
Year1999
Vol126
Start Page3253



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants. gsc expression at 45% epiboly;; 50% epiboly; after shield formation and at 90% epiboly in wild-type and oep mutant embryos.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemesoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionExpression of goosecoid is initiated but not maintained in oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemesoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year1997
Vol124
Start Page327



Evidence for InteractionThe expression of anterior mesendodermal markers (gsc: presumptive hatching glandþprechordal plate; hgg1: presumptive hatching gland; hlx1: prechordal plate) is enlarged in injected embryos, while znot expression in the anterior notochordal domain is abse
Target Genegsc
Upstream Genenomo
Interaction Typerepress
Altered modelblocking the production of protein
Time of Interaction in evidence10
Location of Interaction in evidencemesendoderm
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorChristian Haass
JournalThe EMBO Journal
Year2004
Vol23
Start Page3041



Evidence for InteractionThe expression of anterior mesendodermal markers (gsc: presumptive hatching glandþprechordal plate; hgg1: presumptive hatching gland; hlx1: prechordal plate) is enlarged in injected embryos , while znot expression in the anterior notochordal domain is abs
Target Genegsc
Upstream Genenomo
Interaction Typerepress
Altered modelblocking the production of protein
Time of Interaction in evidence10
Location of Interaction in evidencemesendoderm
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorChristian Haass
JournalThe EMBO Journal
Year2004
Vol23
Start Page3041



Evidence for Interactionduring gastrulation, expression of goosecoid (gsc) becomes reduced in overexpressed embryos.
Target Genegsc
Upstream Genesox32
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence10
Location of Interaction in evidencemesoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHiroyuki Takeda
JournalMechanisms of Development
Year2001
Vol107
Start Page25



Evidence for InteractionIn zygotic oep mutant embryos at 40% epiboly, gsc expression begins to fade in intensity but occupies the same domain as in wild-type embryos. At 50% epiboly and at the beginning of gastrulation, gsc is no longer expressed in zygotic oep mutants.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for InteractionOverexpression of cyclops and squint at different doses leads to the induction of floating head at low doses and the induction of both goosecoid (Gsc) and floating head (Flh) at higher doses
Target GeneGsc
Upstream Genendr2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence5.25
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDevelopment
Year2000
Vol127
Start Page921



Evidence for Interaction36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos.
Target Genegsc
Upstream Geneoep
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence5.25
Location of Interaction in evidencemargin
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorSchier, A. F.
JournalDev Biol
Year2007
Vol304
Start Page525



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of gsca at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn contrast, injection of b-catenin RNA elicited the ectopic expression of boz/dharma, sqt, gsc, and din at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of gsca at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4
Location of Interaction in evidenceYSL
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of gsca at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionIn contrast, injection of b-catenin RNA elicited the ectopic expression of boz/dharma, sqt, gsc, and din at the sphere and shield stages
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence4.66
Location of Interaction in evidencemargin
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionLithium chloride treatment (0.3M LiCl at the 64-256 cell stage), which is known to activate b-catenin signaling, resulted in expanded gsc expression.
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeldrug induce overexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for InteractionInjection of b-catenin RNA elicited the ectopic expression of boz/dharma, sqt, gsc, and din at the sphere and shield stages.
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorHirano, T.
JournalMechanisms of Development
Year2000
Vol91
Start Page293



Evidence for InteractionLithium chloride treatment (0.3M LiCl at the 64-256 cell stage), which is known to activate b-catenin signaling, resulted in the strong inhibition of vega2 expression at the shield stage, whereas gsc expression was expanded.
Target Genegsc
Upstream Genectnnb1
Interaction Typeenhance
Altered modeldrug induce overexpression
Time of Interaction in evidence6
Location of Interaction in evidencemesendoderm
Evidence for Direct 
Binding Site Sequence 
Location of Site 
Last AuthorIgor B. Dawid
Journalgenesis
Year2000
Vol28
Start Page58



Evidence for Interactionzebrafish recessive maternal effect mutant, ichabod, that results in severe anterior and dorsal defects during early development using in-situ hybridization.
Target GeneGsc
Upstream GeneMaternal b-catenin
Interaction Typeenhance
Altered modelmutant
Time of Interaction in evidence5
Location of Interaction in evidenceblastomere
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWeinberg, E. S.
JournalDevelopment
Year2000
Vol127
Start Page3899