Expression Table

Gene Name: gnat2
Description: signal transducer activity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gnat2	Stage	Hours	ZFIN expression range	unspecified
Zygote	1-cell	0
Cleavage	2-cell	0.75
	4-cell	1.0
	8-cell	1.25
	16-cell	1.5
	32-cell	1.75
	64-cell	2.0
Blastula	124-cell	2.25
	256-cell	2.5
	512-cell	2.75
	1k-cell	3.0
	High	3.33
	Oblong	3.66
	Sphere	4.0
	Dome	4.33
	30% epiboly	4.66
Gastrula	50%-epiboly	5.25
	Germ-ring	5.66
	Shield	6.0
	75% epiboly stage	8.0
	90% epiboly	9.0
	bud stage	10.0
Segmentation	1-4 somites	10.33
	5-9 somites	11.66
	10-13 somites	14.0
	14-19 somites	16.0
	20-25 somites	19.0
	26+ somites	22.0
Pharyngula	Prim-5	24.0
	Prim-15	30.0
	Prim-25	36.0
	High-pec	42.0
Hatching	Long-pec	48.0
Hatching	Pec-fin	60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction In crx morphants, only a small ventral-nasal patch of retinal expression was seen, the pineal expression was not significantly changed.

Target Gene gnat2

Upstream Gene crx

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 72

Location of Interaction in evidence retina

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Pamela A. Raymond

Journal Developmental Biology

Year 2004

Vol 269

Start Page 237

Evidence for Interaction Similar to rho and irbp, in crx morphants, expression of gnat1 was reduced in the retina, but not in the pineal (Figs. 7B, F). gnat2 is expressed in cones throughout the retina and in the pineal at 72 hpf (Fig. 7C, G), but in crx morphants, only a small v

Target Gene gnat2

Upstream Gene crx

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 72

Location of Interaction in evidence retina

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Pamela A. Raymond

Journal Developmental Biology

Year 2004

Vol 269

Start Page 237

Evidence for Interaction	In crx morphants, only a small ventral-nasal patch of retinal expression was seen, the pineal expression was not significantly changed.
Target Gene	gnat2
Upstream Gene	crx
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	72
Location of Interaction in evidence	retina
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Pamela A. Raymond
Journal	Developmental Biology
Year	2004
Vol	269
Start Page	237

Evidence for Interaction	Similar to rho and irbp, in crx morphants, expression of gnat1 was reduced in the retina, but not in the pineal (Figs. 7B, F). gnat2 is expressed in cones throughout the retina and in the pineal at 72 hpf (Fig. 7C, G), but in crx morphants, only a small v
Target Gene	gnat2
Upstream Gene	crx
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	72
Location of Interaction in evidence	retina
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Pamela A. Raymond
Journal	Developmental Biology
Year	2004
Vol	269
Start Page	237