Expression Table

Gene Name: gli2a
Description: nucleic acid binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gli2a

Stage

Hours

ZFIN expression range

neural plate

midbrain neural keel

lateral mesoderm

segmental plate

central nervous system

rhombomere 4

rhombomere 5

rhombomere 6

diencephalon

forebrain

hindbrain

midbrain

pharyngeal arch

midbrain hindbrain boundary

choroid fissure

retina

pectoral fin

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Although spinal cord expression of ngn1 appears normal in mutant embryos, expression in DRG precursors is completely eliminated in yot homozygotes. Similarly, expression of ngn1 is absent in DRG precursors in smu mutant embryos (data not shown).

Target Gene neurog1

Upstream Gene gli2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 36

Location of Interaction in evidence DRG precursors

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author David W. Raible

Journal Development

Year 2003

Vol 130

Start Page 5351

Evidence for Interaction	Although spinal cord expression of ngn1 appears normal in mutant embryos, expression in DRG precursors is completely eliminated in yot homozygotes. Similarly, expression of ngn1 is absent in DRG precursors in smu mutant embryos (data not shown).
Target Gene	neurog1
Upstream Gene	gli2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	36
Location of Interaction in evidence	DRG precursors
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	David W. Raible
Journal	Development
Year	2003
Vol	130
Start Page	5351