Expression Table

Gene Name: gdf6a
Description: determination of ventral identity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gdf6a

Stage

Hours

ZFIN expression range

axis

neural plate

mesoderm

ventral mesoderm

endoderm

ectoderm

periderm

tail bud

central nervous system

neural crest

primary neuron

mesenchyme

neural rod

cardiovascular system

eye

neural tube

hatching gland

hypochord

diencephalon

gut

pharyngeal arch 3-7 skeleton

otic vesicle

lateral line primordium

vein

median fin fold

artery

fin

pancreatic bud

ventral mesenchyme

epidermis

retina

spinal cord

apical ectodermal ridge dorsal fin

apical ectodermal ridge median fin fold

dorsal fin musculature

apical ectodermal ridge pectoral fin

pectoral fin musculature

pancreas

dorsal fin

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Injection of radar-MO induced a slight expansion of en1 expression in the somites in comparison with normal embryos.

Target Gene eng1a

Upstream Gene gdf6a

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 28

Location of Interaction in evidence somites

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Hitoshi Okamoto

Journal Current Biology

Year 2005

Vol 15

Start Page 480

Evidence for Interaction	Injection of radar-MO induced a slight expansion of en1 expression in the somites in comparison with normal embryos.
Target Gene	eng1a
Upstream Gene	gdf6a
Interaction Type	repress
Altered model	MASO
Time of Interaction in evidence	28
Location of Interaction in evidence	somites
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Hitoshi Okamoto
Journal	Current Biology
Year	2005
Vol	15
Start Page	480