Expression Table

Gene Name: Gbx1
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gbx1

Stage

Hours

ZFIN expression range

YSL

presumptive brain

blastoderm

margin

presumptive hindbrain

neural plate

ventral mesoderm

hindbrain neural plate

hindbrain neural keel

neural keel

primary neuron

cranial ganglia

neuron

neural tube

rhombomere 3

diencephalon

hindbrain

midbrain

telencephalon

spinal cord

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals.

Target Gene fgf8

Upstream Gene gbx1

Interaction Type enhance

Altered model response to patterning signals

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals. Towards the end of gastrulation, spg ( pou2) expression refines to theMHB territory.At the end of gastrulatio

Target Gene fgf8

Upstream Gene gbx1

Interaction Type enhance

Altered model response to patterning signals

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals. Towards the end of gastrulation, spg ( pou2) expression refines to theMHB territory.At the end of gastrulatio

Target Gene gbx2

Upstream Gene gbx1

Interaction Type enhance

Altered model response to patterning signals

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals. Towards the end of gastrulation, spg ( pou2) expression refines to theMHB territory.At the end of gastrulatio

Target Gene pax2a

Upstream Gene gbx1

Interaction Type enhance

Altered model response to patterning signals

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals.

Target Gene pax2a

Upstream Gene gbx1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals. Towards the end of gastrulation, spg ( pou2) expression refines to theMHB territory.At the end of gastrulatio

Target Gene pou5f1

Upstream Gene gbx1

Interaction Type enhance

Altered model response to patterning signals

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals. Towards the end of gastrulation, spg ( pou2) expression refines to theMHB territory.At the end of gastrulatio

Target Gene wnt1

Upstream Gene gbx1

Interaction Type enhance

Altered model response to patterning signals

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals.

Target Gene wnt1

Upstream Gene gbx1

Interaction Type enhance

Altered model response to patterning signals

Time of Interaction in evidence 10

Location of Interaction in evidence neuroectoderm

Evidence for Direct

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Mechanisms of Development

Year 2003

Vol 120

Start Page 919

Evidence for Interaction	During gastrula stages, three parallel pathways (Pax,Wnt and Fgf) are activated around the otx2/gbx1 interface in response to patterning signals.
Target Gene	fgf8
Upstream Gene	gbx1
Interaction Type	enhance
Altered model	response to patterning signals
Time of Interaction in evidence	10
Location of Interaction in evidence	neuroectoderm
Evidence for Direct
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Mechanisms of Development
Year	2003
Vol	120
Start Page	919