Expression Table

Gene Name: Gata6
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gata6

Stage

Hours

ZFIN expression range

YSL

ventral mesoderm

endoderm

lateral plate mesoderm

heart primordium

gut

primitive heart tube

liver primordium

pancreatic bud

heart

intestinal bulb

liver

swim bladder

anterior pancreatic bud

pancreas

intestinal epithelium

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

?-I

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som

Target Gene gata6

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence endoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Didier Y. R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes, gata6at 36 hpf, 25 hpf, 5-somite stage, and 12-somite stage.

Target Gene gata6

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence endoderm

Evidence for Direct 1, 2

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som

Target Gene gata6

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence cardiovascular system

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Didier Y. R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes, gata6at 36 hpf, 25 hpf, 5-somite stage, and 12-somite stage.

Target Gene gata6

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence cardiovascular system

Evidence for Direct 1, 2

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction Nkx2.5 expression initiates normally in zfMDL MO-injected embryos but, by 10 somites, expression is slightly reduced in 10/13 embryos. At 18 somites, 13/19 injected embryos showed a stronger reduction. GATA-4 and -5 expression is unaffected by zfMDL MO in

Target Gene nkx2.5

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 14

Location of Interaction in evidence cardiac precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction Nkx2.5 expression initiates normally in zfMDL MO-injected embryos but, by 10 somites, expression is slightly reduced in 10/13 embryos. At 18 somites, 13/19 injected embryos showed a stronger reduction. GATA-4 and -5 expression is unaffected by zfMDL MO i

Target Gene nkx2.5

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 14

Location of Interaction in evidence cardiac precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som

Target Gene gata6

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence mesoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Didier Y. R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes, gata6at 36 hpf, 25 hpf, 5-somite stage, and 12-somite stage.

Target Gene gata6

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence mesoderm

Evidence for Direct 1, 2

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction Nkx2.5 expression initiates normally in zfMDL MO-injected embryos but, by 10 somites, expression is slightly reduced in 10/13 embryos. At 18 somites, 13/19 injected embryos showed a stronger reduction. GATA-4 and -5 expression is unaffected by zfMDL MO in

Target Gene nkx2.5

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 16

Location of Interaction in evidence cardiac precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction Nkx2.5 expression initiates normally in zfMDL MO-injected embryos but, by 10 somites, expression is slightly reduced in 10/13 embryos. At 18 somites, 13/19 injected embryos showed a stronger reduction. GATA-4 and -5 expression is unaffected by zfMDL MO i

Target Gene nkx2.5

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 16

Location of Interaction in evidence cardiac precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction Expression of both generalized cardiac markers, such as cardiac myosin light chain 2 (Cmlc2), the transcription factor, Nkx2.5, and the ventricle-specific marker, ventricular myosin heavy chain (Vmhc), were downregulated in the vast majority of embryos.

Target Gene cmlc1

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence cardiac precursors

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction Expression of both generalized cardiac markers, such as cardiac myosin light chain 2 (Cmlc2), the transcription factor, Nkx2.5, and the ventricle-speci®c marker, ventricular myosin heavy chain (Vmhc), were downregulated in the vast majority of embryos.

Target Gene nkx2.5

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence cardia bifida

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction Expression of both generalized cardiac markers, such as cardiac myosin light chain 2 (Cmlc2), the transcription factor, Nkx2.5, and the ventricle-specific marker, ventricular myosin heavy chain (Vmhc), were downregulated in the vast majority of embryos.

Target Gene nkx2.5

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence cardiac precursors

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction Expression of both generalized cardiac markers, such as cardiac myosin light chain 2 (Cmlc2), the transcription factor, Nkx2.5, and the ventricle-speci®c marker, ventricular myosin heavy chain (Vmhc), were downregulated in the vast majority of embryos.

Target Gene nkx2.5

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence heart

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction The ventricle-specific marker, ventricular myosin heavy chain (Vmhc), were downregulated in the gata6 morphan injected embryos.

Target Gene vmhc

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence cardia bifida

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction Expression of both generalized cardiac markers, such as cardiac myosin light chain 2 (Cmlc2), the transcription factor, Nkx2.5, and the ventricle-specific marker, ventricular myosin heavy chain (Vmhc), were downregulated in the vast majority of embryos.

Target Gene vmhc

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence cardiac precursors

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction The ventricle-specific marker, ventricular myosin heavy chain (Vmhc), were downregulated in the gata6 morphan injected embryos.

Target Gene vmhc

Upstream Gene gata6

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence heart

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal The EMBO Journal

Year 2003

Vol 22

Start Page 4260

Evidence for Interaction	overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som
Target Gene	gata6
Upstream Gene	gata5
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	14
Location of Interaction in evidence	endoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Didier Y. R. Stainier
Journal	Genes & Development
Year	1999
Vol	13
Start Page	2983

Evidence for Interaction	Nkx2.5 expression initiates normally in zfMDL MO-injected embryos but, by 10 somites, expression is slightly reduced in 10/13 embryos. At 18 somites, 13/19 injected embryos showed a stronger reduction. GATA-4 and -5 expression is unaffected by zfMDL MO in
Target Gene	nkx2.5
Upstream Gene	gata6
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	14
Location of Interaction in evidence	cardiac precursors
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	The EMBO Journal
Year	2003
Vol	22
Start Page	4260

Evidence for Interaction	Expression of both generalized cardiac markers, such as cardiac myosin light chain 2 (Cmlc2), the transcription factor, Nkx2.5, and the ventricle-specific marker, ventricular myosin heavy chain (Vmhc), were downregulated in the vast majority of embryos.
Target Gene	cmlc1
Upstream Gene	gata6
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	26
Location of Interaction in evidence	cardiac precursors
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	The EMBO Journal
Year	2003
Vol	22
Start Page	4260

Evidence for Interaction	The ventricle-specific marker, ventricular myosin heavy chain (Vmhc), were downregulated in the gata6 morphan injected embryos.
Target Gene	vmhc
Upstream Gene	gata6
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	26
Location of Interaction in evidence	cardia bifida
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	The EMBO Journal
Year	2003
Vol	22
Start Page	4260