| gata5 | Stage | Hours | ZFIN expression range | marginal blastomere | YSL | presumptive endoderm | margin | hypoblast | mesoderm | ventral mesoderm | endoderm | lateral mesoderm | anterior lateral plate mesoderm | lateral plate mesoderm | heart primordium | posterior lateral plate mesoderm | gut | pharynx | primitive heart tube | heart | intestinal bulb | liver | pancreas | endocardium | whole organism | unspecified |
| Zygote | 1-cell | 0 | ||||||||||||||||||||||||
| Cleavage | 2-cell | 0.75 | ||||||||||||||||||||||||
| 4-cell | 1.0 | |||||||||||||||||||||||||
| 8-cell | 1.25 | |||||||||||||||||||||||||
| 16-cell | 1.5 | |||||||||||||||||||||||||
| 32-cell | 1.75 | |||||||||||||||||||||||||
| 64-cell | 2.0 | |||||||||||||||||||||||||
| Blastula | 124-cell | 2.25 | ||||||||||||||||||||||||
| 256-cell | 2.5 | |||||||||||||||||||||||||
| 512-cell | 2.75 | |||||||||||||||||||||||||
| 1k-cell | 3.0 | |||||||||||||||||||||||||
| High | 3.33 | |||||||||||||||||||||||||
| Oblong | 3.66 | |||||||||||||||||||||||||
| Sphere | 4.0 | |||||||||||||||||||||||||
| Dome | 4.33 | |||||||||||||||||||||||||
| 30% epiboly | 4.66 | I | ||||||||||||||||||||||||
| Gastrula | 50%-epiboly | 5.25 | ! | ! | ||||||||||||||||||||||
| Germ-ring | 5.66 | ! | ! | |||||||||||||||||||||||
| Shield | 6.0 | ! | ! | |||||||||||||||||||||||
| 75% epiboly stage | 8.0 | I | ||||||||||||||||||||||||
| 90% epiboly | 9.0 | |||||||||||||||||||||||||
| bud stage | 10.0 | |||||||||||||||||||||||||
| Segmentation | 1-4 somites | 10.33 | I | |||||||||||||||||||||||
| 5-9 somites | 11.66 | I | I | |||||||||||||||||||||||
| 10-13 somites | 14.0 | I | ||||||||||||||||||||||||
| 14-19 somites | 16.0 | I | I | |||||||||||||||||||||||
| 20-25 somites | 19.0 | I | I | ! | ! | |||||||||||||||||||||
| 26+ somites | 22.0 | I | I | ! | ||||||||||||||||||||||
| Pharyngula | Prim-5 | 24.0 | ||||||||||||||||||||||||
| Prim-15 | 30.0 | |||||||||||||||||||||||||
| Prim-25 | 36.0 | |||||||||||||||||||||||||
| High-pec | 42.0 | |||||||||||||||||||||||||
| Hatching | Long-pec | 48.0 | ? | ? | ||||||||||||||||||||||
| Pec-fin | 60.0 | I | I | I |
| Evidence for Interaction | Coexpression of eomes with bon and gata5 had a dramatic and synergistic effect on the expression of the endodermal differentiation marker sox17, which was strongly induced in marginal as well as nonmarginal blastomeres while coexpression of eomes with eit |
| Target Gene | gata5 |
| Upstream Gene | eomes |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 4 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | David Kimelman |
| Journal | Developmental Cell |
| Year | 2005 |
| Vol | 9 |
| Start Page | 523 |
| Evidence for Interaction | The greatest increase in luciferase activity was seen when p(1476)cas-luc was coinjected with eomes, bon, and gata5 RNAs. |
| Target Gene | sox32 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 4 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | David Kimelman |
| Journal | Developmental Cell |
| Year | 2005 |
| Vol | 9 |
| Start Page | 523 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of bon, gata5, and eomes strongly induces expression of sox17 throughout the embryo, including cells at the animal pole. |
| Target Gene | sox17 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | David Kimelman |
| Journal | Developmental Cell |
| Year | 2005 |
| Vol | 9 |
| Start Page | 523 |
| Evidence for Interaction | overexpression of bon, gata5, and eomes induces expression of the endodermal determinant sox32(cas) in marginal and nonmarginal blastomeres. |
| Target Gene | sox32 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | David Kimelman |
| Journal | Developmental Cell |
| Year | 2005 |
| Vol | 9 |
| Start Page | 523 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | swr/bmp2b mutants lack ventral, mesodermal expression of gata5. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | In wild-type embryos at 80% epiboly (8.3 hpf), gata5 is expressed in large, flat endodermal cells predominantly on the dorsal side and smaller, rounder mesodermal cells on the ventral side . Swr/bmp2b mutants lack ventral, mesodermal expression of gata5. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | In wild-type embryos at 80% epiboly (8.3 hpf), gata5 is expressed in large, flat endodermal cells predominantly on the dorsal side and smaller, rounder mesodermal cells on the ventral side . Swr/bmp2b mutants lack ventral, mesodermal expression of gata5. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | flat endodermal cells |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | gata-5 expression in endodermal progenitor cells, but not in the YSL, is strongly reduced or absent in the 226D7- (sox32)MO-injected embryos at 80%-epiboly. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive endoderm |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Mechanisms of Development |
| Year | 2001 |
| Vol | 107 |
| Start Page | 25 |
| Evidence for Interaction | Gata-5 expression in endodermal progenitor cells, but not in the YSL, is strongly reduced or absent in the Sox32(226D7)-MO-injected embryos at 80%-epiboly. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive endoderm |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Mechanisms of Development |
| Year | 2001 |
| Vol | 107 |
| Start Page | 25 |
| Evidence for Interaction | swr/bmp2b mutants lack ventral, mesodermal expression of gata5. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | anterior LPM |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | In wild-type embryos at 80% epiboly (8.3 hpf), gata5 is expressed in large, flat endodermal cells predominantly on the dorsal side and smaller, rounder mesodermal cells on the ventral side . Swr/bmp2b mutants lack ventral, mesodermal expression of gata5. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | anterior LPM |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Compared with the lacZ injected siblings, injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17, foxa2 and gata6. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric Rosa |
| Journal | GENES & DEVELOPMENT |
| Year | 2001 |
| Vol | 15 |
| Start Page | 1487 |
| Evidence for Interaction | Compared with the lacZ injected siblings, injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17, foxa2 and gata6. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric Rosa |
| Journal | GENES & DEVELOPMENT |
| Year | 2001 |
| Vol | 15 |
| Start Page | 1487 |
| Evidence for Interaction | Compared with the lacZ injected siblings, injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17, foxa2 and gata6. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric Rosa |
| Journal | GENES & DEVELOPMENT |
| Year | 2001 |
| Vol | 15 |
| Start Page | 1487 |
| Evidence for Interaction | Compared with the lacZ injected siblings , injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17 , foxa2 and gata5. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric M. Rosa |
| Journal | Development |
| Year | 2002 |
| Vol | 129 |
| Start Page | 275 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Compared with the lacZ injected siblings , injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17 , foxa2 and gata5. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric M. Rosa |
| Journal | Development |
| Year | 2002 |
| Vol | 129 |
| Start Page | 275 |
| Evidence for Interaction | Compared with the lacZ injected siblings , injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17 , foxa2 and gata5. |
| Target Gene | gata5 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric M. Rosa |
| Journal | Development |
| Year | 2002 |
| Vol | 129 |
| Start Page | 275 |
| Evidence for Interaction | overexpression of gata5 can restore bilateral nkx2.5 expression in swr/bmp2b mutants. |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1, cmlc2, nkx2.5, gata4, and gata6 in wild-type embryos and fau mutant siblings at 36 hpf, 25 hpf, 5-somite stage ( |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | Injection of 50 pg of wild-type gata5 mRNA in wild-type embryos leads to expanded expression of nkx2.5 as shown previously (Reiter et al., 1999). overexpression of gata5 can restore bilateral nkx2.5 expression in swr/bmp2b mutants. |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1, cmlc2, nkx2.5, gata4, and gata6 in wild-type embryos and fau mutant siblings at 36 hpf, 25 hpf, 5-somite stage ( |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | Injection of 50 pg of wild-type gata5 mRNA in wild-type embryos leads to expanded expression of nkx2.5 as shown previously (Reiter et al., 1999). overexpression of gata5 can restore bilateral nkx2.5 expression in swr/bmp2b mutants. |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 can restore bilateral nkx2.5 expression in swr/bmp2b mutants. |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1, cmlc2, nkx2.5, gata4, and gata6 in wild-type embryos and fau mutant siblings at 36 hpf, 25 hpf, 5-somite stage ( |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | Injection of 50 pg of wild-type gata5 mRNA in wild-type embryos leads to expanded expression of nkx2.5 as shown previously (Reiter et al., 1999). overexpression of gata5 can restore bilateral nkx2.5 expression in swr/bmp2b mutants. |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1, cmlc2, nkx2.5, gata4, and gata6 in wild-type embryos and fau mutant siblings at 36 hpf, 25 hpf, 5-somite stage ( |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | Injection of 50 pg of wild-type gata5 mRNA in wild-type embryos leads to expanded expression of nkx2.5 as shown previously (Reiter et al., 1999). overexpression of gata5 can restore bilateral nkx2.5 expression in swr/bmp2b mutants. |
| Target Gene | nkx2.5 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | At the 12-somite stage, gata5 is expressed in the anterior LPM. Swr/bmp2b mutants lack gata5 expression in the anterior LPM but exhibit gata5 expression in a few cells of unknown identity underneath the head. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | At the 12-somite stage (15 hpf), swr/bmp2b mutants lack gata5 expression in the anterior LPM. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | flat endodermal cells |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | At the 12-somite stage, gata5 is expressed in the anterior LPM. Swr/bmp2b mutants lack gata5 expression in the anterior LPM but exhibit gata5 expression in a few cells of unknown identity underneath the head. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | flat endodermal cells |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | gata6 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes, gata6at 36 hpf, 25 hpf, 5-somite stage, and 12-somite stage. |
| Target Gene | gata6 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2 , nkx2.5, gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-somite stag |
| Target Gene | gata4 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | gata4 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | gata6 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes, gata6at 36 hpf, 25 hpf, 5-somite stage, and 12-somite stage. |
| Target Gene | gata6 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | cardiovascular system |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | At the 12-somite stage, gata5 is expressed in the anterior LPM. Swr/bmp2b mutants lack gata5 expression in the anterior LPM but exhibit gata5 expression in a few cells of unknown identity underneath the head. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | anterior LPM |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | At the 12-somite stage (15 hpf), swr/bmp2b mutants lack gata5 expression in the anterior LPM. |
| Target Gene | gata5 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | anterior LPM |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2 , nkx2.5, gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-somite stag |
| Target Gene | gata4 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | gata4 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | gata6 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes, gata6at 36 hpf, 25 hpf, 5-somite stage, and 12-somite stage. |
| Target Gene | gata6 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | Zoep mutants do not express gata5 in the anterior LPM at the 12-somite stage, but do express gata5 more posteriorly. |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | Zoep mutants exhibit decreased gata5 expression in the germ ring by 50% epiboly. At 80% epiboly, gata5 expression in Zoep mutants is limited to the YSL and a few cells within the blastoderm. Zoep mutants do not express gata5 in the anterior LPM at the |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | myl7 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | heart |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 restores cmlc1 expression in Zoep mutants. |
| Target Gene | cmlc1 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 26 |
| Location of Interaction in evidence | heart |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | overexpression of gata5 restores cmlc1 expression in Zoep mutants at 26 hp. |
| Target Gene | cmlc1 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 26 |
| Location of Interaction in evidence | heart |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Developmental Biology |
| Year | 2001 |
| Vol | 234 |
| Start Page | 330 |
| Evidence for Interaction | In the Gata4 morphant, the pattern of Gata5 expression in the intestinal rod appears relatively diffuse, when compared with controls at 28 hpf. |
| Target Gene | gata5 |
| Upstream Gene | gata4 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 28 |
| Location of Interaction in evidence | intestin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Todd Evans |
| Journal | Development and disease |
| Year | 2005 |
| Vol | 132 |
| Start Page | 4005 |
| Evidence for Interaction | In the Gata4 morphant, the pattern of Gata5 expression in the intestinal rod appears relatively diffuse, when compared with controls at 28 hpf. |
| Target Gene | gata5 |
| Upstream Gene | gata4 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 28 |
| Location of Interaction in evidence | intestin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Todd Evans |
| Journal | Development and disease |
| Year | 2005 |
| Vol | 132 |
| Start Page | 4005 |
| Evidence for Interaction | The gata5 gene is expressed at a low level in the developing intestine and heart, and this is increased significantly in the heart tube of morphant embryos at 28 hpf. |
| Target Gene | gata5 |
| Upstream Gene | gata4 |
| Interaction Type | repress |
| Altered model | MASO |
| Time of Interaction in evidence | 28 |
| Location of Interaction in evidence | heart |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Todd Evans |
| Journal | Development and disease |
| Year | 2005 |
| Vol | 132 |
| Start Page | 4005 |
| Evidence for Interaction | The gata5 gene is expressed at a low level in the developing intestine and heart, and this is increased significantly in the heart tube of morphant embryos at 28 hpf. |
| Target Gene | gata5 |
| Upstream Gene | gata4 |
| Interaction Type | repress |
| Altered model | MASO |
| Time of Interaction in evidence | 28 |
| Location of Interaction in evidence | heart |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Todd Evans |
| Journal | Development and disease |
| Year | 2005 |
| Vol | 132 |
| Start Page | 4005 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of cmlc1. |
| Target Gene | cmlc1 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | heart |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y.R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som |
| Target Gene | cmlc1 |
| Upstream Gene | gata5 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | heart |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Didier Y. R. Stainier |
| Journal | Genes & Development |
| Year | 1999 |
| Vol | 13 |
| Start Page | 2983 |
| Evidence for Interaction | 27 genes that are expressed in the dorsal margin in wild type, but lack margin expression in MZoep mutants. |
| Target Gene | gata5 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 304 |
| Start Page | 525 |