Expression Table

Gene Name: Gata4
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gata4

Stage

Hours

ZFIN expression range

mesoderm

lateral mesoderm

tail bud

anterior lateral plate mesoderm

lateral plate mesoderm

heart primordium

gut

liver primordium

heart

liver

intestinal epithelium

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Expression of the zinc-finger transcription factor gata4 is also clearly reduced in mutant embryos at the 3- to 6- somite stage .

Target Gene gata4

Upstream Gene fgf8

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence cardiovascular system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2000

Vol 127

Start Page 225

Evidence for Interaction Expression of the zinc-finger transcription factor gata4 is also clearly reduced in mutant embryos at the 3- to 6- somite stage .

Target Gene gata4

Upstream Gene fgf8

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2000

Vol 127

Start Page 225

Evidence for Interaction Expression of the zinc-finger transcription factor gata4 is also clearly reduced in mutant embryos at the 3- to 6- somite stage.

Target Gene gata4

Upstream Gene fgf8

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence cardiovascular system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2000

Vol 127

Start Page 225

Evidence for Interaction Expression of the zinc-finger transcription factor gata4 is also clearly reduced in mutant embryos at the 3- to 6- somite stage.

Target Gene gata4

Upstream Gene fgf8

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2000

Vol 127

Start Page 225

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2 , nkx2.5, gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-somite stag

Target Gene gata4

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence cardiovascular system

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som

Target Gene gata4

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence cardiovascular system

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Didier Y. R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2 , nkx2.5, gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-somite stag

Target Gene gata4

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence mesoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2(myl7) , nkx2.5 , gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-som

Target Gene gata4

Upstream Gene gata5

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence mesoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Didier Y. R. Stainier

Journal Genes & Development

Year 1999

Vol 13

Start Page 2983

Evidence for Interaction In the Gata4 morphant, the pattern of Gata5 expression in the intestinal rod appears relatively diffuse, when compared with controls at 28 hpf.

Target Gene gata5

Upstream Gene gata4

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 28

Location of Interaction in evidence intestin

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Todd Evans

Journal Development and disease

Year 2005

Vol 132

Start Page 4005

Evidence for Interaction The gata5 gene is expressed at a low level in the developing intestine and heart, and this is increased significantly in the heart tube of morphant embryos at 28 hpf.

Target Gene gata5

Upstream Gene gata4

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 28

Location of Interaction in evidence heart

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Todd Evans

Journal Development and disease

Year 2005

Vol 132

Start Page 4005

Evidence for Interaction In embryos in which Bmp signaling was blocked at 18 hpf, gata4 and gata6 expression was greatly reduced in the liver region at 29-30 hpf.

Target Gene gata4

Upstream Gene bmp2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 30

Location of Interaction in evidence liver progenitors

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Didier Y. R. Stainier

Journal Development

Year 2007

Vol 134

Start Page 2041

Evidence for Interaction In embryos in which Bmp signaling was blocked at 18 hpf, gata4 and gata6 expression was greatly reduced in the liver region at 29-30 hpf.

Target Gene gata4

Upstream Gene bmp2b

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 30

Location of Interaction in evidence liver progenitors

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Didier Y. R. Stainier

Journal Development

Year 2007

Vol 134

Start Page 2041

Evidence for Interaction Site-dirrected mutation of multiple T-box binding elements in Gata4 enhancer significatly decreased promoter expression in the heart.

Target Gene gata4

Upstream Gene tbx24

Interaction Type enhance

Altered model GFP reporter essay in-vivo

Time of Interaction in evidence 48

Location of Interaction in evidence heart

Evidence for Direct 6

Binding Site Sequence T-box binding site

Location of Site 8-11kb 5'UTR

Last Author Todd Evans

Journal Development Biology

Year 2004

Vol 267

Start Page 490

Evidence for Interaction	Expression of the zinc-finger transcription factor gata4 is also clearly reduced in mutant embryos at the 3- to 6- somite stage .
Target Gene	gata4
Upstream Gene	fgf8
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	cardiovascular system
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	2000
Vol	127
Start Page	225

Evidence for Interaction	overexpression of gata5 rescues cardiac fusion in fautm236a mutants and leads to ectopic expression of myocardial genes. Expression of cmlc1 , cmlc2 , nkx2.5, gata4 , and gata6 in wild-type embryos and fau mutant siblings at 36 hpf , 25 hpf, 5-somite stag
Target Gene	gata4
Upstream Gene	gata5
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	14
Location of Interaction in evidence	cardiovascular system
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Didier Y.R. Stainier
Journal	Genes & Development
Year	1999
Vol	13
Start Page	2983

Evidence for Interaction	In the Gata4 morphant, the pattern of Gata5 expression in the intestinal rod appears relatively diffuse, when compared with controls at 28 hpf.
Target Gene	gata5
Upstream Gene	gata4
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	28
Location of Interaction in evidence	intestin
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Todd Evans
Journal	Development and disease
Year	2005
Vol	132
Start Page	4005

Evidence for Interaction	The gata5 gene is expressed at a low level in the developing intestine and heart, and this is increased significantly in the heart tube of morphant embryos at 28 hpf.
Target Gene	gata5
Upstream Gene	gata4
Interaction Type	repress
Altered model	MASO
Time of Interaction in evidence	28
Location of Interaction in evidence	heart
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Todd Evans
Journal	Development and disease
Year	2005
Vol	132
Start Page	4005

Evidence for Interaction	In embryos in which Bmp signaling was blocked at 18 hpf, gata4 and gata6 expression was greatly reduced in the liver region at 29-30 hpf.
Target Gene	gata4
Upstream Gene	bmp2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	30
Location of Interaction in evidence	liver progenitors
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Didier Y. R. Stainier
Journal	Development
Year	2007
Vol	134
Start Page	2041

Evidence for Interaction	Site-dirrected mutation of multiple T-box binding elements in Gata4 enhancer significatly decreased promoter expression in the heart.
Target Gene	gata4
Upstream Gene	tbx24
Interaction Type	enhance
Altered model	GFP reporter essay in-vivo
Time of Interaction in evidence	48
Location of Interaction in evidence	heart
Evidence for Direct	6
Binding Site Sequence	T-box binding site
Location of Site	8-11kb 5'UTR
Last Author	Todd Evans
Journal	Development Biology
Year	2004
Vol	267
Start Page	490