Expression Table

Gene Name: Gata3
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gata3

Stage

Hours

ZFIN expression range

presumptive epidermis

non neural ectoderm

ectoderm

otic placode

periderm

cranial ganglia

floor plate

lens placode

neural tube

pronephric duct

hindbrain

pharyngeal arch 3-7 skeleton

otic vesicle

lens

trigeminal ganglion

optic tectum

retina

spinal cord

tegmentum

serotonergic neuron

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in the fgf3 injected embryo.

Target Gene gata3

Upstream Gene fgf3

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence ectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Developmental Biology

Year 2002

Vol 244

Start Page 9

Evidence for Interaction Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo.

Target Gene gata3

Upstream Gene fgf3

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence ectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Developmental Biology

Year 2002

Vol 244

Start Page 9

Evidence for Interaction Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in the fgf3 injected embryo.

Target Gene gata3

Upstream Gene fgf3

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence epiblast

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Developmental Biology

Year 2002

Vol 244

Start Page 9

Evidence for Interaction Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo.

Target Gene gata3

Upstream Gene fgf3

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence epiblast

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Developmental Biology

Year 2002

Vol 244

Start Page 9

Evidence for Interaction Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo.

Target Gene gata3

Upstream Gene fgf3

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 10

Location of Interaction in evidence ectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Developmental Biology

Year 2002

Vol 244

Start Page 9

Evidence for Interaction Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo.

Target Gene gata3

Upstream Gene fgf3

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 10

Location of Interaction in evidence epiblast

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Developmental Biology

Year 2002

Vol 244

Start Page 9

Evidence for Interaction The gata3-positive cluster was significantly enlarged in the mutant bonnie and clyde/mixer(bon) from 24 hpf onward.

Target Gene gata3

Upstream Gene bon

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 26

Location of Interaction in evidence brain

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Laure Bally-Cuif

Journal DEVELOPMENTAL DYNAMICS

Year 2003

Vol 227

Start Page 524

Evidence for Interaction The gata3 expression was significantly enlarged in the mutant bonnie and clyde/mixer from 24 hpf onward.The overall shape of the gata3 cluster was maintained, indicating no anisotropy in the increase in cell number along the AP or DV axes.

Target Gene gata3

Upstream Gene bon

Interaction Type repress

Altered model mutant embryos

Time of Interaction in evidence 26

Location of Interaction in evidence brain

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Laure Bally-Cuif

Journal DEVELOPMENTAL DYNAMICS

Year 2003

Vol 227

Start Page 524

Evidence for Interaction	Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in the fgf3 injected embryo.
Target Gene	gata3
Upstream Gene	fgf3
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	ectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Hiroyuki Takeda
Journal	Developmental Biology
Year	2002
Vol	244
Start Page	9

Evidence for Interaction	The gata3-positive cluster was significantly enlarged in the mutant bonnie and clyde/mixer(bon) from 24 hpf onward.
Target Gene	gata3
Upstream Gene	bon
Interaction Type	repress
Altered model	mutant embryos
Time of Interaction in evidence	26
Location of Interaction in evidence	brain
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Laure Bally-Cuif
Journal	DEVELOPMENTAL DYNAMICS
Year	2003
Vol	227
Start Page	524

Evidence for Interaction	The gata3 expression was significantly enlarged in the mutant bonnie and clyde/mixer from 24 hpf onward.The overall shape of the gata3 cluster was maintained, indicating no anisotropy in the increase in cell number along the AP or DV axes.
Target Gene	gata3
Upstream Gene	bon
Interaction Type	repress
Altered model	mutant embryos
Time of Interaction in evidence	26
Location of Interaction in evidence	brain
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Laure Bally-Cuif
Journal	DEVELOPMENTAL DYNAMICS
Year	2003
Vol	227
Start Page	524