Expression Table

Gene Name: Gata2
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gata2

Stage

Hours

ZFIN expression range

presumptive ectoderm

presumptive epidermis

presumptive blood

non neural ectoderm

ectoderm

lateral mesoderm

lateral plate mesoderm

cranial ganglia

hematopoietic system

neuron

neural tube

hindbrain

pharyngeal arch

intermediate cell mass of mesoderm

otic vesicle

trunk vasculature

axial vasculature

ventricular zone

optic tectum

retina

tegmentum

intersegmental vessel

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Injection with 4 ng of chd-MO led to expansion of gata2 expression.

Target Gene gata2

Upstream Gene chordin

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 6

Location of Interaction in evidence EVL

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction Dorsalizing activity of fgf17b is mediated by chd. Injection with 1 pg of fgf17b mRNA inhibited expression of ventral markers bmp2, bmp4, eve1, and gata2, whereas injection with 4 ng of chd-MO led to expansion of these markers. When coinjected with the s

Target Gene gata2

Upstream Gene chordin

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 6

Location of Interaction in evidence EVL

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction Injection with 4 ng of chd-MO led to expansion of gata2 expression.

Target Gene gata2

Upstream Gene chordin

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 6

Location of Interaction in evidence epiblast

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction Dorsalizing activity of fgf17b is mediated by chd. Injection with 1 pg of fgf17b mRNA inhibited expression of ventral markers bmp2, bmp4, eve1, and gata2, whereas injection with 4 ng of chd-MO led to expansion of these markers. When coinjected with the s

Target Gene gata2

Upstream Gene chordin

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 6

Location of Interaction in evidence epiblast

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction In MZ-laf/alk8 mutant embryos gata2 expression is absent.

Target Gene gata2

Upstream Gene alk8

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence epiblast

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Mary C. Mullins

Journal Development

Year 2001

Vol 128

Start Page 859

Evidence for Interaction gata2 showed remarkable reduction of expression on the ventral side of the fgf17b injected embryos.

Target Gene gata2

Upstream Gene fgf17b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence ectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction gata2 expression showed remarkable reduction of expression on the ventral side of the fgf17b mRNA injected embryos.

Target Gene gata2

Upstream Gene fgf17b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence ectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction gata2 showed remarkable reduction of expression on the ventral side of the fgf17b injected embryos.

Target Gene gata2

Upstream Gene fgf17b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence mesoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction gata2 expression showed remarkable reduction of expression on the ventral side of the fgf17b mRNA injected embryos.

Target Gene gata2

Upstream Gene fgf17b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence mesoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction Gata2, which is normally expressed in the ventral half of the animal-pole region, expands dorsally and increases at 80%-epiboly after injection of 226D7 RNAs

Target Gene gata2

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence ventral half of the animal-pole region (mesoderm)

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Mechanisms of Development

Year 2001

Vol 107

Start Page 25

Evidence for Interaction gata2 expands dorsally and increases at 80%-epiboly after injection of 226D7 (sox32) RNAs.

Target Gene gata2

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence ventral half of the animal-pole region (mesoderm)

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Mechanisms of Development

Year 2001

Vol 107

Start Page 25

Evidence for Interaction Gata2 expression is initially absent in tbx16, appears at 5s-7s. Nonneural ectodermal gata2 expression in tbx16 mutant.

Target Gene gata2

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction Gata2 expression is initially absent in spt, then appears at 5s-7s.

Target Gene gata2

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence hematopoietic system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction At nine somites gata2 is expressed in two bilateral stripes of prospective blood in wild-type embryo,in ntl mutants the stripes of gata2 are less separated posteriorly.

Target Gene gata2

Upstream Gene ntl

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence hematopoietic system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Solnica-Krezel1

Journal Development

Year 2004

Vol 131

Start Page 203

Evidence for Interaction At nine somites, myoD is expressed in the somites and adaxial cells, and gta2 is expressed in two bilateral stripes of prospective blood in wild-type embryos. In ntl mutants, adaxial cell expression of myoD is lost and the stripes of gta2 are less separat

Target Gene gata2

Upstream Gene ntl

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence hematopoietic system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author David Kimelman

Journal Development

Year 2003

Vol 131

Start Page 203

Evidence for Interaction Gata2 expression is initially absent in tbx16, appears at 5s-7s. Nonneural ectodermal gata2 expression in tbx16 mutant.

Target Gene gata2

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence blood

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction gata2 expression is initially absent in spt, appears at 5s-7s.

Target Gene gata2

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence hematopoietic system

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction zebrafish recessive maternal effect mutant, ichabod, that results in severe anterior and dorsal defects during early development using in-situ hybridization.

Target Gene gata2

Upstream Gene Maternal b-catenin

Interaction Type repress

Altered model mutant

Time of Interaction in evidence 5

Location of Interaction in evidence blastomere

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Weinberg, E. S.

Journal Development

Year 2000

Vol 127

Start Page 3899

Evidence for Interaction	Injection with 4 ng of chd-MO led to expansion of gata2 expression.
Target Gene	gata2
Upstream Gene	chordin
Interaction Type	repress
Altered model	MASO
Time of Interaction in evidence	6
Location of Interaction in evidence	EVL
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130

Evidence for Interaction	Dorsalizing activity of fgf17b is mediated by chd. Injection with 1 pg of fgf17b mRNA inhibited expression of ventral markers bmp2, bmp4, eve1, and gata2, whereas injection with 4 ng of chd-MO led to expansion of these markers. When coinjected with the s
Target Gene	gata2
Upstream Gene	chordin
Interaction Type	repress
Altered model	MASO
Time of Interaction in evidence	6
Location of Interaction in evidence	EVL
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130

Evidence for Interaction	In MZ-laf/alk8 mutant embryos gata2 expression is absent.
Target Gene	gata2
Upstream Gene	alk8
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	8
Location of Interaction in evidence	epiblast
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Mary C. Mullins
Journal	Development
Year	2001
Vol	128
Start Page	859

Evidence for Interaction	gata2 showed remarkable reduction of expression on the ventral side of the fgf17b injected embryos.
Target Gene	gata2
Upstream Gene	fgf17b
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	ectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130

Evidence for Interaction	gata2 expression showed remarkable reduction of expression on the ventral side of the fgf17b mRNA injected embryos.
Target Gene	gata2
Upstream Gene	fgf17b
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	ectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130

Evidence for Interaction	Gata2, which is normally expressed in the ventral half of the animal-pole region, expands dorsally and increases at 80%-epiboly after injection of 226D7 RNAs
Target Gene	gata2
Upstream Gene	sox32
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	ventral half of the animal-pole region (mesoderm)
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Hiroyuki Takeda
Journal	Mechanisms of Development
Year	2001
Vol	107
Start Page	25

Evidence for Interaction	gata2 expands dorsally and increases at 80%-epiboly after injection of 226D7 (sox32) RNAs.
Target Gene	gata2
Upstream Gene	sox32
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	ventral half of the animal-pole region (mesoderm)
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Hiroyuki Takeda
Journal	Mechanisms of Development
Year	2001
Vol	107
Start Page	25

Evidence for Interaction	Gata2 expression is initially absent in tbx16, appears at 5s-7s. Nonneural ectodermal gata2 expression in tbx16 mutant.
Target Gene	gata2
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	blood
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	At nine somites gata2 is expressed in two bilateral stripes of prospective blood in wild-type embryo,in ntl mutants the stripes of gata2 are less separated posteriorly.
Target Gene	gata2
Upstream Gene	ntl
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	11.66
Location of Interaction in evidence	hematopoietic system
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Solnica-Krezel1
Journal	Development
Year	2004
Vol	131
Start Page	203

Evidence for Interaction	At nine somites, myoD is expressed in the somites and adaxial cells, and gta2 is expressed in two bilateral stripes of prospective blood in wild-type embryos. In ntl mutants, adaxial cell expression of myoD is lost and the stripes of gta2 are less separat
Target Gene	gata2
Upstream Gene	ntl
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	11.66
Location of Interaction in evidence	hematopoietic system
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	David Kimelman
Journal	Development
Year	2003
Vol	131
Start Page	203

Evidence for Interaction	zebrafish recessive maternal effect mutant, ichabod, that results in severe anterior and dorsal defects during early development using in-situ hybridization.
Target Gene	gata2
Upstream Gene	Maternal b-catenin
Interaction Type	repress
Altered model	mutant
Time of Interaction in evidence	5
Location of Interaction in evidence	blastomere
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Weinberg, E. S.
Journal	Development
Year	2000
Vol	127
Start Page	3899