Expression Table

Gene Name: gadd45b
Description: response to methylmercury
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

gadd45b

Stage

Hours

ZFIN expression range

YSL

EVL

axis

shield

presumptive segmental plate

mesoderm

ventral mesoderm

periderm

polster

segmental plate

tail bud

somite

olfactory placode

pronephric duct

hypothalamus

retina

peripheral olfactory organ

cleithrum

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction overexpression of gadd45 genes reduced the expression of segmentation genes her1.

Target Gene her1

Upstream Gene gadd45b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 11.66

Location of Interaction in evidence somite

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction overexpression of gadd45? genes affects somite segmentation.Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side.

Target Gene mespa

Upstream Gene gadd45b

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 11.66

Location of Interaction in evidence somite

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction overexpression of gadd45? genes affects somite segmentation. Whole-mount in situ hybridization with probes shown at the upper right. Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side.

Target Gene myod

Upstream Gene gadd45b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 11.66

Location of Interaction in evidence somite

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction overexpression of gadd45 genes reduced the expression of segmentation genes her1.

Target Gene her1

Upstream Gene gadd45b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence somite

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction overexpression of gadd45 genes affects somite segmentation. Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side ,whereas ntl expression was not changed.

Target Gene her1

Upstream Gene gadd45b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence somite

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction overexpression of gadd45 genes affects somite segmentation. Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side ,whereas ntl expression was not changed.

Target Gene mespa

Upstream Gene gadd45b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence somite

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction overexpression of gadd45? genes affects somite segmentation.Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side.

Target Gene mespa

Upstream Gene gadd45b

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence somite

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction overexpression of gadd45 genes affects somite segmentation. Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side ,whereas ntl expression was not changed.

Target Gene myod

Upstream Gene gadd45b

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence somite

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Igor B. Dawid

Journal PNAS

Year 2004

Vol 102

Start Page 361

Evidence for Interaction 36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos.

Target Gene gadd45b

Upstream Gene oep

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.25

Location of Interaction in evidence margin

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Schier, A. F.

Journal Dev Biol

Year 2007

Vol 304

Start Page 525

Evidence for Interaction	overexpression of gadd45 genes reduced the expression of segmentation genes her1.
Target Gene	her1
Upstream Gene	gadd45b
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	11.66
Location of Interaction in evidence	somite
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Igor B. Dawid
Journal	PNAS
Year	2004
Vol	102
Start Page	361

Evidence for Interaction	overexpression of gadd45? genes affects somite segmentation.Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side.
Target Gene	mespa
Upstream Gene	gadd45b
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	11.66
Location of Interaction in evidence	somite
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Igor B. Dawid
Journal	PNAS
Year	2004
Vol	102
Start Page	361

Evidence for Interaction	overexpression of gadd45 genes affects somite segmentation. Expression of segmentation genes (mesp-a, her1, and MyoD) was severely reduced in the injected side ,whereas ntl expression was not changed.
Target Gene	her1
Upstream Gene	gadd45b
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	14
Location of Interaction in evidence	somite
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Igor B. Dawid
Journal	PNAS
Year	2004
Vol	102
Start Page	361

Evidence for Interaction	36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos.
Target Gene	gadd45b
Upstream Gene	oep
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.25
Location of Interaction in evidence	margin
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Schier, A. F.
Journal	Dev Biol
Year	2007
Vol	304
Start Page	525