Expression Table

Gene Name: FST
Description: molecular function unknown
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

fst

Stage

Hours

ZFIN expression range

axis

presumptive segmental plate

neural plate

presumptive cephalic mesoderm

mesoderm

ectoderm

midbrain neural keel

segmental plate

central nervous system

head

somite

head mesenchyme

eye

neural tube

diencephalon

forebrain

hindbrain

midbrain

optic stalk

pharyngeal arch 3-7 skeleton

otic vesicle

cerebellum

optic tectum

retina

spinal cord

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction The expression of follistatin (fst) was enhanced on the dorsal side of the fgf17b injected late gastrulae.

Target Gene fst

Upstream Gene fgf17b

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 6

Location of Interaction in evidence mesendoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction The expression of fst, another dorsal marker, was also enhanced on the dorsal side of the injected late gastrulae.

Target Gene fst

Upstream Gene fgf17b

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 6

Location of Interaction in evidence mesendoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Anming Meng

Journal Developmental Biology

Year 2004

Vol 271

Start Page 130

Evidence for Interaction Neither mesp-a nor mesp-b is expressed in fss mutants during segmentation.

Target Gene mespb

Upstream Gene fst

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Development

Year 2000

Vol 127

Start Page 1691

Evidence for Interaction Neither mesp-a nor mesp-b is expressed in fss mutants during segmentation.

Target Gene mespa

Upstream Gene fst

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Development

Year 2000

Vol 127

Start Page 1691

Evidence for Interaction Expression of follistatin is reduced in the chordino mutant embryo.

Target Gene fst

Upstream Gene chordin

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 9

Location of Interaction in evidence mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Matthias Hammerschmidt

Journal Developmental Biology

Year 1998

Vol 204

Start Page 488

Evidence for Interaction Expression of follistatin is reduced in the chordino mutant embryo, spanning the dorsalmost 30° of the embryonic ircumference, compared to 150° in wild-type sibling embryos

Target Gene fst

Upstream Gene chordin

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 9

Location of Interaction in evidence mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Matthias Hammerschmidt

Journal Developmental Biology

Year 1998

Vol 204

Start Page 488

Evidence for Interaction Neither mesp-a nor mesp-b is expressed in fss mutants during segmentation.

Target Gene mespb

Upstream Gene fst

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 9

Location of Interaction in evidence

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Development

Year 2000

Vol 127

Start Page 1691

Evidence for Interaction Neither mesp-a nor mesp-b is expressed in fss mutants during segmentation.

Target Gene mespa

Upstream Gene fst

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 9

Location of Interaction in evidence

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Development

Year 2000

Vol 127

Start Page 1691

Evidence for Interaction Neither mesp-a nor mesp-b is expressed in fss mutants during segmentation.

Target Gene mespa

Upstream Gene fst

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence somite

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Development

Year 2000

Vol 127

Start Page 1691

Evidence for Interaction Neither mesp-a nor mesp-b is expressed in fss mutants during segmentation.

Target Gene mespb

Upstream Gene fst

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence somite

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Hiroyuki Takeda

Journal Development

Year 2000

Vol 127

Start Page 1691

Evidence for Interaction	The expression of follistatin (fst) was enhanced on the dorsal side of the fgf17b injected late gastrulae.
Target Gene	fst
Upstream Gene	fgf17b
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	6
Location of Interaction in evidence	mesendoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130

Evidence for Interaction	The expression of fst, another dorsal marker, was also enhanced on the dorsal side of the injected late gastrulae.
Target Gene	fst
Upstream Gene	fgf17b
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	6
Location of Interaction in evidence	mesendoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Anming Meng
Journal	Developmental Biology
Year	2004
Vol	271
Start Page	130

Evidence for Interaction	Neither mesp-a nor mesp-b is expressed in fss mutants during segmentation.
Target Gene	mespb
Upstream Gene	fst
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	8
Location of Interaction in evidence
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Hiroyuki Takeda
Journal	Development
Year	2000
Vol	127
Start Page	1691

Evidence for Interaction	Expression of follistatin is reduced in the chordino mutant embryo.
Target Gene	fst
Upstream Gene	chordin
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	9
Location of Interaction in evidence	mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Matthias Hammerschmidt
Journal	Developmental Biology
Year	1998
Vol	204
Start Page	488