Expression Table

Gene Name: foxA3
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

foxa3

Stage

Hours

ZFIN expression range

marginal blastomere

YSL

blastoderm

margin

shield

endoderm

tail bud

notochord

pancreas primordium

hatching gland

gut

pharyngeal arch

pharyngeal pouch

liver primordium

pancreatic bud

liver

swim bladder

pancreas

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction foxa3 is not expressed in the endoderm of SOX32-MO injected embryos, while notochordal expression in the tail is retained.

Target Gene foxa3

Upstream Gene sox32

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 24

Location of Interaction in evidence endoderm

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Victoria E. Prince

Journal Development

Year 2005

Vol 133

Start Page 949

Evidence for Interaction 27 genes that are expressed in the dorsal margin in wild type, but lack margin expression in MZoep mutants.

Target Gene foxA3

Upstream Gene oep

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.25

Location of Interaction in evidence margin

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Schier, A. F.

Journal Dev Biol

Year 2007

Vol 304

Start Page 525

Evidence for Interaction	foxa3 is not expressed in the endoderm of SOX32-MO injected embryos, while notochordal expression in the tail is retained.
Target Gene	foxa3
Upstream Gene	sox32
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	24
Location of Interaction in evidence	endoderm
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Victoria E. Prince
Journal	Development
Year	2005
Vol	133
Start Page	949

Evidence for Interaction	27 genes that are expressed in the dorsal margin in wild type, but lack margin expression in MZoep mutants.
Target Gene	foxA3
Upstream Gene	oep
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.25
Location of Interaction in evidence	margin
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Schier, A. F.
Journal	Dev Biol
Year	2007
Vol	304
Start Page	525