| foxa2 | Stage | Hours | ZFIN expression range | presumptive endoderm | margin | axis | hypoblast | presumptive floor plate | neural plate | axial mesoderm | prechordal plate | endoderm | neuroectoderm | hindbrain neural keel | neural keel | tail bud | notochord | presumptive diencephalon | lateral floor plate | medial floor plate | floor plate | neural tube | pharyngeal endoderm | brain | gut | midbrain | floor plate neural tube | floor plate spinal cord | spinal cord | zona limitans intrathalamica | liver | cranial nerve II | exocrine pancreas | whole organism | unspecified |
| Zygote | 1-cell | 0 | |||||||||||||||||||||||||||||||||
| Cleavage | 2-cell | 0.75 | |||||||||||||||||||||||||||||||||
| 4-cell | 1.0 | ||||||||||||||||||||||||||||||||||
| 8-cell | 1.25 | ||||||||||||||||||||||||||||||||||
| 16-cell | 1.5 | ||||||||||||||||||||||||||||||||||
| 32-cell | 1.75 | ||||||||||||||||||||||||||||||||||
| 64-cell | 2.0 | ||||||||||||||||||||||||||||||||||
| Blastula | 124-cell | 2.25 | |||||||||||||||||||||||||||||||||
| 256-cell | 2.5 | ||||||||||||||||||||||||||||||||||
| 512-cell | 2.75 | ||||||||||||||||||||||||||||||||||
| 1k-cell | 3.0 | ||||||||||||||||||||||||||||||||||
| High | 3.33 | ||||||||||||||||||||||||||||||||||
| Oblong | 3.66 | ||||||||||||||||||||||||||||||||||
| Sphere | 4.0 | ||||||||||||||||||||||||||||||||||
| Dome | 4.33 | ||||||||||||||||||||||||||||||||||
| 30% epiboly | 4.66 | ||||||||||||||||||||||||||||||||||
| Gastrula | 50%-epiboly | 5.25 | |||||||||||||||||||||||||||||||||
| Germ-ring | 5.66 | I | I | ||||||||||||||||||||||||||||||||
| Shield | 6.0 | ||||||||||||||||||||||||||||||||||
| 75% epiboly stage | 8.0 | ||||||||||||||||||||||||||||||||||
| 90% epiboly | 9.0 | I | I | ||||||||||||||||||||||||||||||||
| bud stage | 10.0 | I | I | ||||||||||||||||||||||||||||||||
| Segmentation | 1-4 somites | 10.33 | |||||||||||||||||||||||||||||||||
| 5-9 somites | 11.66 | ! | |||||||||||||||||||||||||||||||||
| 10-13 somites | 14.0 | I | |||||||||||||||||||||||||||||||||
| 14-19 somites | 16.0 | ||||||||||||||||||||||||||||||||||
| 20-25 somites | 19.0 | I | I | I | I | I | |||||||||||||||||||||||||||||
| 26+ somites | 22.0 | I | I | I | |||||||||||||||||||||||||||||||
| Pharyngula | Prim-5 | 24.0 | I | I | I | ||||||||||||||||||||||||||||||
| Prim-15 | 30.0 | I | I | I | I | I | |||||||||||||||||||||||||||||
| Prim-25 | 36.0 | I | I | I | I | I | I | I | |||||||||||||||||||||||||||
| High-pec | 42.0 | I | I | I | I | I | I | I | |||||||||||||||||||||||||||
| Hatching | Long-pec | 48.0 | |||||||||||||||||||||||||||||||||
| Pec-fin | 60.0 | I | I | I | I |
| Evidence for Interaction | The reduction of endodermal expression of axial/foxa2 and sox17 in sqt-/-; cyc+/+ and sqt-/-; cyc+/- embryos is particularly apparent on the dorsal margin |
| Target Gene | foxa2 |
| Upstream Gene | ndr1 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | William S. Talbot |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 1837 |
| Evidence for Interaction | The reduction of endodermal expression of axial/foxa2 and sox17 in sqt-/-; cyc+/+ and sqt-/-; cyc+/- embryos is particularly apparent on the dorsal margin |
| Target Gene | foxa2 |
| Upstream Gene | ndr1 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | William S. Talbot |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 1837 |
| Evidence for Interaction | Injected embryos lacked endodermal cells expressing foxA2 whereas foxA2 expression was unaffected in the midline of the body axis. |
| Target Gene | foxa2 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric Rosa |
| Journal | GENES & DEVELOPMENT |
| Year | 2001 |
| Vol | 15 |
| Start Page | 1487 |
| Evidence for Interaction | overexpression of cas mRNA is sufficient to induce foxa2. |
| Target Gene | foxa2 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | David Kimelman |
| Journal | Developmental Cell |
| Year | 2005 |
| Vol | 9 |
| Start Page | 523 |
| Evidence for Interaction | overexpression of cas mRNA is sufficient to induce foxa2. |
| Target Gene | foxa2 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | David Kimelman |
| Journal | Developmental Cell |
| Year | 2005 |
| Vol | 9 |
| Start Page | 523 |
| Evidence for Interaction | injected embryos lacked cells expressing foxA2 ; sox17 expression was completely abolished by injection of cas (sox32)-MO . |
| Target Gene | foxa2 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric Rosa |
| Journal | GENES & DEVELOPMENT |
| Year | 2001 |
| Vol | 15 |
| Start Page | 1487 |
| Evidence for Interaction | The reduction of endodermal expression of axial/foxa2 and sox17 in sqt-/-; cyc+/+ and sqt-/-; cyc+/- embryos is particularly apparent on the dorsal margin |
| Target Gene | foxa2 |
| Upstream Gene | ndr1 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | William S. Talbot |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 1837 |
| Evidence for Interaction | The reduction of endodermal expression of axial/foxa2 and sox17 in sqt-/-; cyc+/+ and sqt-/-; cyc+/- embryos is particularly apparent on the dorsal margin |
| Target Gene | foxa2 |
| Upstream Gene | ndr1 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | William S. Talbot |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 1837 |
| Evidence for Interaction | Compared with the lacZ injected siblings, injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17, foxa2 and gata5. |
| Target Gene | foxa2 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric M. Rosa |
| Journal | Development |
| Year | 2002 |
| Vol | 129 |
| Start Page | 275 |
| Evidence for Interaction | Compared with the lacZ injected siblings, injection of cas RNA at the 8/16-cell stage induces expression of endodermal markers sox17, foxa2 and gata5. |
| Target Gene | foxa2 |
| Upstream Gene | sox32 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Frédéric Rosa |
| Journal | GENES & DEVELOPMENT |
| Year | 2001 |
| Vol | 15 |
| Start Page | 1487 |
| Evidence for Interaction | In homozygous bozm168 mutant embryos, the dorsal mesendoderm expression of axial is very reduced; however, the paraxial endoderm expression does not appear affected . |
| Target Gene | foxa2 |
| Upstream Gene | dharma |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Developmental Biology |
| Year | 1999 |
| Vol | 215 |
| Start Page | 190 |
| Evidence for Interaction | In homozygous bozm168 (dharma) mutant embryos, the dorsal mesendoderm expression of Foxa2 is very reduced. |
| Target Gene | foxa2 |
| Upstream Gene | dharma |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Developmental Biology |
| Year | 1999 |
| Vol | 215 |
| Start Page | 190 |
| Evidence for Interaction | Axial (foxa2) expression is reduced in the midline and abolished in endodermal precursors of injected embryos. |
| Target Gene | foxa2 |
| Upstream Gene | lft2 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 1999 |
| Vol | 126 |
| Start Page | 3253 |
| Evidence for Interaction | axial expression is reduced in the midline and abolished in endodermal precursors of injected embryos. |
| Target Gene | foxa2 |
| Upstream Gene | lft2 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | endoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 1999 |
| Vol | 126 |
| Start Page | 3253 |
| Evidence for Interaction | Axial (foxa2) expression is reduced in the midline and abolished in endodermal precursors of injected embryos. |
| Target Gene | foxa2 |
| Upstream Gene | lft2 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 1999 |
| Vol | 126 |
| Start Page | 3253 |
| Evidence for Interaction | axial expression is reduced in the midline and abolished in endodermal precursors of injected embryos. |
| Target Gene | foxa2 |
| Upstream Gene | lft2 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | H. Joseph Yost |
| Journal | Development |
| Year | 1999 |
| Vol | 126 |
| Start Page | 3253 |
| Evidence for Interaction | Correspondingly, the expression of axial (foxa2) and shh, two genes expressed in both the developing prechordal plate and ventral neuroectoderm is reduced anteriorly. |
| Target Gene | foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | expression of axial and shh, two genes expressed in both the developing prechordal plate and ventral neuroectoderm is reduced anteriorly. |
| Target Gene | foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Correspondingly, the expression of axial (foxa2) and shh, two genes expressed in both the developing prechordal plate and ventral neuroectoderm is reduced anteriorly. |
| Target Gene | foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | expression of axial and shh, two genes expressed in both the developing prechordal plate and ventral neuroectoderm is reduced anteriorly. |
| Target Gene | foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Misexpression of axial (Foxa2) leads to ectopic activation of snic hedgehog expression in the zebrafish. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | hindbrain |
| Evidence for Direct | 6 |
| Binding Site Sequence | 1. TGTTTT 2. ATAACA |
| Location of Site | 1. -102~ -107 2. -91~ -96 |
| Last Author | U Strahle |
| Journal | EMBO Journal |
| Year | 1997 |
| Vol | 16 |
| Start Page | 3955 |
| Evidence for Interaction | Misexpression of axial (Foxa2) leads to ectopic activation of snic hedgehog expression in the zebrafish. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | midbrain |
| Evidence for Direct | 6 |
| Binding Site Sequence | 1. TGTTTT 2. ATAACA |
| Location of Site | 1. -102~ -107 2. -91~ -96 |
| Last Author | U Strahle |
| Journal | EMBO Journal |
| Year | 1997 |
| Vol | 16 |
| Start Page | 3955 |
| Evidence for Interaction | Misexpression of axial leads to ectopic activation of snic hedgehog expression in the zebrafish. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | axis |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | U Strahle |
| Journal | EMBO Journal |
| Year | 1997 |
| Vol | 16 |
| Start Page | 3955 |
| Evidence for Interaction | In embryos homozyzous for the cyclops mutation, foxa2 expression is normal in mesendodermal cells but is absent from the ventral neural tube. |
| Target Gene | foxa2 |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | ventral neural tube |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | P.W. Ingham |
| Journal | GENES & DEVELOPMENT |
| Year | 1993 |
| Vol | 7 |
| Start Page | 1436 |
| Evidence for Interaction | Axial (Foxa2) expression is extended dorsally in the posterior diencephalon. |
| Target Gene | foxa2 |
| Upstream Gene | shha |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | posterior diencephalon |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Thomas Gerster |
| Journal | Development |
| Year | 1996 |
| Vol | 122 |
| Start Page | 1769 |
| Evidence for Interaction | hlx-1 expression in the prechordal plate and the ventralmost midline ectoderm is not significantly altered by shh overexpression. |
| Target Gene | foxa2 |
| Upstream Gene | shha |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | posterior diencephalon |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Thomas Gerster |
| Journal | Development |
| Year | 1996 |
| Vol | 122 |
| Start Page | 1769 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Transverse sections through the spinal cord of wild-type and mol-/- embryos showing changes in floorplate expression of various genes in the mutants. |
| Target Gene | nkx2.2a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Transverse sections through the spinal cord of wild-type and mol-/- embryos showing changes in floorplate expression of various genes in the mutants. |
| Target Gene | nkx2.2a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Transverse sections through the spinal cord of wild-type and mol-/- embryos showing changes in floorplate expression of various genes in the mutants. |
| Target Gene | nkx2.2b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Transverse sections through the spinal cord of wild-type and mol-/- embryos showing changes in floorplate expression of various genes in the mutants. |
| Target Gene | nkx2.2b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Transverse sections through the spinal cord of wild-type and mol-/- embryos showing changes in floorplate expression of various genes in the mutants. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Transverse sections through the spinal cord of wild-type and mol-/- embryos showing changes in floorplate expression of various genes in the mutants. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Misexpression of axial leads to ectopic activation of snic hedgehog expression in the zebrafish. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | hindbrain |
| Evidence for Direct | 6 |
| Binding Site Sequence | 1. TGTTTT 2. ATAACA |
| Location of Site | 1. -102~ -107 2. -91~ -96 |
| Last Author | U Strahle |
| Journal | EMBO Journal |
| Year | 1997 |
| Vol | 16 |
| Start Page | 3955 |
| Evidence for Interaction | Misexpression of axial leads to ectopic activation of snic hedgehog expression in the zebrafish. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | midbrain |
| Evidence for Direct | 6 |
| Binding Site Sequence | 1. TGTTTT 2. ATAACA |
| Location of Site | 1. -102~ -107 2. -91~ -96 |
| Last Author | U Strahle |
| Journal | EMBO Journal |
| Year | 1997 |
| Vol | 16 |
| Start Page | 3955 |
| Evidence for Interaction | Floorplate differentiation fails in FoxA2 mutant embryos. |
| Target Gene | arx |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Netrin1 (ntn1b) is ectopically activated by foxa2, and expression of foxa2 leads to rescue of floor plate differentiation in mutant embryos that are deficient in Cyclops signalling. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | U Strahle |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 252 |
| Start Page | 1 |
| Evidence for Interaction | Netrin1 (ntn1b) is ectopically activated by foxa2, and expression of foxa2 leads to rescue of floor plate differentiation in mutant embryos that are deficient in Cyclops signalling. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | U Strahle |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 252 |
| Start Page | 1 |
| Evidence for Interaction | Expression of PKI elicited strong ectopic activation of the floor plate marker genes netrin1 and axial/HNF3 b. |
| Target Gene | foxa2 |
| Upstream Gene | shha |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Uwe Strähle |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 3889 |
| Evidence for Interaction | Expression of PKI elicited strong ectopic activation of the floor plate marker genes netrin1 (88.7%, n=71) and axial/HNF3 b (83.9%, n=93). |
| Target Gene | foxa2 |
| Upstream Gene | shha |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Uwe Strähle |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 3889 |
| Evidence for Interaction | Lateral view of wildtype and mutant embryos at 24 hpf showing FP markers such as shh and axial are present at reduced levels in smo embryos. |
| Target Gene | foxa2 |
| Upstream Gene | smo |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Nancy Hopkins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 2385 |
| Evidence for Interaction | Lateral view of wildtype and mutant embryos at 24 hpf showing FP markers such as shh and axial are present at reduced levels in smo embryos. |
| Target Gene | foxa2 |
| Upstream Gene | smo |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Nancy Hopkins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 2385 |
| Evidence for Interaction | Lateral view of wildtype and mutant embryos at 24 hpf showing FP markers such as shh and axial are present at reduced levels in smo embryos. Close up dorsal view of axial expression in embryos at 24 hpf, indicating the lack of LFP and the presence of MFP |
| Target Gene | foxa2 |
| Upstream Gene | smo |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Nancy Hopkins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 2385 |
| Evidence for Interaction | Close up dorsal view of axial expression in embryos at 24 hpf, indicating the lack of LFP and the presence of MFP in the mutant. |
| Target Gene | foxa2 |
| Upstream Gene | smo |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Nancy Hopkins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 2385 |
| Evidence for Interaction | Misexpression of axial leads to ectopic activation of snic hedgehog expression in the zebrafish. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | U Strahle |
| Journal | EMBO Journal |
| Year | 1997 |
| Vol | 16 |
| Start Page | 3955 |
| Evidence for Interaction | Expression of PKI elicited strong ectopic activation of the floor plate marker genes netrin1 and axial/HNF3 b. |
| Target Gene | foxa2 |
| Upstream Gene | shha |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | spinal cord |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Uwe Strähle |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 3889 |
| Evidence for Interaction | Expression of PKI elicited strong ectopic activation of the floor plate marker genes netrin1 (88.7%, n=71) and axial/HNF3 b (83.9%, n=93). |
| Target Gene | foxa2 |
| Upstream Gene | shha |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | spinal cord |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Uwe Strähle |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 3889 |
| Evidence for Interaction | Expression of nk2.2a is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | nkx2.2a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | hindbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of nk2.2a and ntn1 is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | nkx2.2a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | hindbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of ntn1b is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | hindbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of nk2.2a and ntn1 (ntn1b) is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | hindbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of nk2.2a is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | nkx2.2a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | midbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of nk2.2a and ntn1 is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | nkx2.2a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | midbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of ntn1b is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | midbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of nk2.2a and ntn1 (ntn1b) is expanded dorsally in the midbrain and hindbrain neural tube of mol-/- embryos, but is reduced in smu-/- embryos. |
| Target Gene | ntn1b |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | midbrain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Floorplate differentiation fails in mol-/- embryos and the expression of col2a1a was reduced. |
| Target Gene | col2a1a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Floorplate differentiation fails in mol-/- but not in smu-/- embryos. |
| Target Gene | col2a1a |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | shha |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | shhb |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | shhb |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of axial is markedly reduced in mutant embryos at 35 hpf. Axial can be seen in a small region in the ventral midbrain. |
| Target Gene | foxa2 |
| Upstream Gene | smo |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Nancy Hopkins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 2385 |
| Evidence for Interaction | Expression of axial is markedly reduced in mutant embryos at 35 hpf. |
| Target Gene | foxa2 |
| Upstream Gene | smo |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 36 |
| Location of Interaction in evidence | floorplate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Nancy Hopkins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 2385 |
| Evidence for Interaction | Expression of axial in the pharyngeal endoderm is normal in wild-type but not in oep mutant embryos. Expression in the brain of oep mutants is also severely affected as compared to wild-type. |
| Target Gene | Foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 50 |
| Location of Interaction in evidence | pharyngeal endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Expression of axial in the pharyngeal endoderm is normal in wild-type but not in oep mutant embryos at 51 hpf. |
| Target Gene | foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 50 |
| Location of Interaction in evidence | pharyngeal endoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Expression of axial in the pharyngeal endoderm is normal in wild-type but not in oep mutant embryos. Expression in the brain of oep mutants is also severely affected as compared to wild-type. |
| Target Gene | Foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 50 |
| Location of Interaction in evidence | brain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Expression of axial in the pharyngeal endoderm is normal in wild-type but not in oep mutant embryos at 51 hpf. |
| Target Gene | foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 50 |
| Location of Interaction in evidence | brain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | foxa1 |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 60 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants. |
| Target Gene | foxa1 |
| Upstream Gene | foxa2 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 60 |
| Location of Interaction in evidence | medial floor plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | SW Wilson |
| Journal | Development |
| Year | 2005 |
| Vol | 132 |
| Start Page | 645 |
| Evidence for Interaction | 27 genes that are expressed in the dorsal margin in wild type, but lack margin expression in MZoep mutants. |
| Target Gene | foxa2 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | blastomere |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 304 |
| Start Page | 525 |