Expression Table

Gene Name: FoxA1
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

foxa1

Stage

Hours

ZFIN expression range

endoderm

notochord

chordo neural hinge

floor plate

hypochord

pronephric duct

diencephalon

forebrain

gut

midbrain

pancreatic bud

liver

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction During mid-somitogenesis fkd7 (foxa1) is expressed in the endoderm as well as in the floor plate and hypochord. In bon mutants, there are only a few fkd7-expressing endodermal cells, whereas expression in the floor plate and hypochord is normal.

Target Gene foxa1

Upstream Gene bon

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence endoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 2000

Vol 14

Start Page 1279

Evidence for Interaction In bon mutants, there are only a few foxa1-expressing endodermal cells, whereas expression in the floor plate and hypochord is normal.

Target Gene foxa1

Upstream Gene bon

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence CNS

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 2000

Vol 14

Start Page 1279

Evidence for Interaction In bon mutants, there are only a few foxa1-expressing endodermal cells, whereas expression in the floor plate and hypochord is normal.

Target Gene foxa1

Upstream Gene bon

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence notochord

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Didier Y.R. Stainier

Journal Genes & Development

Year 2000

Vol 14

Start Page 1279

Evidence for Interaction Fkd7 (FoxA1)-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas (Sox32).

Target Gene foxa1

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 24

Location of Interaction in evidence digestive system

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Frédéric M. Rosa

Journal Development

Year 2002

Vol 129

Start Page 275

Evidence for Interaction fkd7-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas.

Target Gene foxa1

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 24

Location of Interaction in evidence digestive system

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Frédéric Rosa

Journal GENES & DEVELOPMENT

Year 2001

Vol 15

Start Page 1487

Evidence for Interaction Fkd7 (FoxA1)-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas (Sox32).

Target Gene foxa1

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 24

Location of Interaction in evidence endoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Frédéric M. Rosa

Journal Development

Year 2002

Vol 129

Start Page 275

Evidence for Interaction fkd7-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas.

Target Gene foxa1

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 24

Location of Interaction in evidence endoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Frédéric Rosa

Journal GENES & DEVELOPMENT

Year 2001

Vol 15

Start Page 1487

Evidence for Interaction Fkd7 (FoxA1)-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas (Sox32).

Target Gene foxa1

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 24

Location of Interaction in evidence notochord

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Frédéric M. Rosa

Journal Development

Year 2002

Vol 129

Start Page 275

Evidence for Interaction fkd7-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas.

Target Gene foxa1

Upstream Gene sox32

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 24

Location of Interaction in evidence notochord

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Frédéric Rosa

Journal GENES & DEVELOPMENT

Year 2001

Vol 15

Start Page 1487

Evidence for Interaction Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants.

Target Gene foxa1

Upstream Gene foxa2

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 60

Location of Interaction in evidence medial floor plate

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author SW Wilson

Journal Development

Year 2005

Vol 132

Start Page 645

Evidence for Interaction	During mid-somitogenesis fkd7 (foxa1) is expressed in the endoderm as well as in the floor plate and hypochord. In bon mutants, there are only a few fkd7-expressing endodermal cells, whereas expression in the floor plate and hypochord is normal.
Target Gene	foxa1
Upstream Gene	bon
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	endoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Didier Y.R. Stainier
Journal	Genes & Development
Year	2000
Vol	14
Start Page	1279

Evidence for Interaction	In bon mutants, there are only a few foxa1-expressing endodermal cells, whereas expression in the floor plate and hypochord is normal.
Target Gene	foxa1
Upstream Gene	bon
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	CNS
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Didier Y.R. Stainier
Journal	Genes & Development
Year	2000
Vol	14
Start Page	1279

Evidence for Interaction	Fkd7 (FoxA1)-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas (Sox32).
Target Gene	foxa1
Upstream Gene	sox32
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	24
Location of Interaction in evidence	digestive system
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Frédéric M. Rosa
Journal	Development
Year	2002
Vol	129
Start Page	275

Evidence for Interaction	fkd7-positive endodermal structures were also restored in bon/mixer embryos overexpressing cas.
Target Gene	foxa1
Upstream Gene	sox32
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	24
Location of Interaction in evidence	digestive system
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Frédéric Rosa
Journal	GENES & DEVELOPMENT
Year	2001
Vol	15
Start Page	1487

Evidence for Interaction	Expression of regulatory genes expressed in the MFP of wildtype embryos is not maintained in mol-/- mutants.
Target Gene	foxa1
Upstream Gene	foxa2
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	60
Location of Interaction in evidence	medial floor plate
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	SW Wilson
Journal	Development
Year	2005
Vol	132
Start Page	645