Expression Table

Gene Name: flil
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

fli1

Stage

Hours

ZFIN expression range

mesoderm

ventral mesoderm

lateral mesoderm

intermediate mesoderm

head

lateral plate mesoderm

anterior lateral mesoderm

posterior lateral mesoderm

heart primordium

cardiovascular system

pharyngeal arch 3-7 skeleton

intermediate cell mass of mesoderm

otic vesicle

heart rudiment

trunk vasculature

vasculature

vein

blood island

artery

axial vasculature

dorsal aorta

posterior cardinal vein

caudal vein plexus

cranial vasculature

median axial vein

intersegmental vessel

dorsal longitudinal anastomotic vessel

mandibular arch

pharyngeal arch 2 skeleton

segmental intercostal artery

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Cdx4 mRNA injected embryos showed ectopic fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.

Target Gene fli1

Upstream Gene cdx4

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 11.66

Location of Interaction in evidence blood precursors

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction The cdx4 mRNA injected embryos showed ectopic fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.

Target Gene fli1

Upstream Gene cdx4

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 11.66

Location of Interaction in evidence blood precursors

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Cdx4 mRNA injected embryos showed ectopic fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.

Target Gene fli1

Upstream Gene cdx4

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 11.66

Location of Interaction in evidence midline

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction The cdx4 mRNA injected embryos showed ectopic fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.

Target Gene fli1

Upstream Gene cdx4

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 11.66

Location of Interaction in evidence midline

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Surprisingly, 12-20% of the injected embryos showed ectopic scl, gata1 and fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.

Target Gene fli1

Upstream Gene cdx4

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence blood precursors

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction Surprisingly, 12-20% of the injected embryos showed ectopic scl, gata1 and fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.

Target Gene fli1

Upstream Gene cdx4

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 14

Location of Interaction in evidence midline

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Leonard I. Zon

Journal Nature

Year 2003

Vol 425

Start Page 18

Evidence for Interaction In MO-injected embryos, the distinct line of DA precursor cells at the midline was barely evident. In addition, fli1 expression in PCV precursors was severely reduced.

Target Gene fli1

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 16

Location of Interaction in evidence posterior cardinal vein precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction In tal1 MO-injected embryos fli1 expression in PCV precursors was severely reduced.

Target Gene fli1

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 16

Location of Interaction in evidence posterior cardinal vein precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction In 26-hpf scl morphant embryos, expression of fli1 and the later endothelial marker tie147 in the midline where the 2 axial vessels develop was significantly down-regulated.

Target Gene fli1

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence axial vessels

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction In 26-hpf tal1 morphant embryos, expression of fli1 in the midline was significantly down-regulated.

Target Gene fli1

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence axial vessels

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction	Cdx4 mRNA injected embryos showed ectopic fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.
Target Gene	fli1
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	11.66
Location of Interaction in evidence	blood precursors
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18

Evidence for Interaction	The cdx4 mRNA injected embryos showed ectopic fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.
Target Gene	fli1
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	11.66
Location of Interaction in evidence	blood precursors
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18

Evidence for Interaction	Surprisingly, 12-20% of the injected embryos showed ectopic scl, gata1 and fli1 expression near the midline in a stripe that ran parallel to the endogenous blood precursors.
Target Gene	fli1
Upstream Gene	cdx4
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	14
Location of Interaction in evidence	blood precursors
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Leonard I. Zon
Journal	Nature
Year	2003
Vol	425
Start Page	18

Evidence for Interaction	In MO-injected embryos, the distinct line of DA precursor cells at the midline was barely evident. In addition, fli1 expression in PCV precursors was severely reduced.
Target Gene	fli1
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	16
Location of Interaction in evidence	posterior cardinal vein precursors
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	In tal1 MO-injected embryos fli1 expression in PCV precursors was severely reduced.
Target Gene	fli1
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	16
Location of Interaction in evidence	posterior cardinal vein precursors
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	In 26-hpf scl morphant embryos, expression of fli1 and the later endothelial marker tie147 in the midline where the 2 axial vessels develop was significantly down-regulated.
Target Gene	fli1
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	26
Location of Interaction in evidence	axial vessels
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	In 26-hpf tal1 morphant embryos, expression of fli1 in the midline was significantly down-regulated.
Target Gene	fli1
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	26
Location of Interaction in evidence	axial vessels
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9