| flh | Stage | Hours | ZFIN expression range | presumptive shield | shield | neural plate | axial mesoderm | prechordal plate | notochord | tail | diencephalon | epiphysis | telencephalon | unspecified |
| Zygote | 1-cell | 0 | ||||||||||||
| Cleavage | 2-cell | 0.75 | ||||||||||||
| 4-cell | 1.0 | |||||||||||||
| 8-cell | 1.25 | |||||||||||||
| 16-cell | 1.5 | |||||||||||||
| 32-cell | 1.75 | |||||||||||||
| 64-cell | 2.0 | |||||||||||||
| Blastula | 124-cell | 2.25 | ||||||||||||
| 256-cell | 2.5 | |||||||||||||
| 512-cell | 2.75 | |||||||||||||
| 1k-cell | 3.0 | |||||||||||||
| High | 3.33 | |||||||||||||
| Oblong | 3.66 | |||||||||||||
| Sphere | 4.0 | |||||||||||||
| Dome | 4.33 | |||||||||||||
| 30% epiboly | 4.66 | I | I | |||||||||||
| Gastrula | 50%-epiboly | 5.25 | ||||||||||||
| Germ-ring | 5.66 | I | I | |||||||||||
| Shield | 6.0 | |||||||||||||
| 75% epiboly stage | 8.0 | |||||||||||||
| 90% epiboly | 9.0 | |||||||||||||
| bud stage | 10.0 | |||||||||||||
| Segmentation | 1-4 somites | 10.33 | I | I | I | |||||||||
| 5-9 somites | 11.66 | I | I | |||||||||||
| 10-13 somites | 14.0 | |||||||||||||
| 14-19 somites | 16.0 | |||||||||||||
| 20-25 somites | 19.0 | ?-I | I | |||||||||||
| 26+ somites | 22.0 | ?-I | I | |||||||||||
| Pharyngula | Prim-5 | 24.0 | ? | |||||||||||
| Prim-15 | 30.0 | |||||||||||||
| Prim-25 | 36.0 | |||||||||||||
| High-pec | 42.0 | |||||||||||||
| Hatching | Long-pec | 48.0 | ||||||||||||
| Pec-fin | 60.0 |
| Evidence for Interaction | The flh expression domain is expanded toward the margin in zygotic oep mutants, occupying tiers 1-9. while flh is expressed ectopically at the margin. |
| Target Gene | flh |
| Upstream Gene | oep |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | Overexpression of cyclops and squint at different doses leads to the induction of floating head at low doses and the induction of both goosecoid (Gsc) and floating head (Flh) at higher doses |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | Overexpression of cyclops and squint at different doses leads to the induction of floating head at low doses and the induction of both goosecoid (Gsc) and floating head (Flh) at higher doses |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | The flh expression domain is expanded toward the margin in zygotic oep mutants, occupying tiers 1-9. while flh is expressed ectopically at the margin. |
| Target Gene | flh |
| Upstream Gene | oep |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | The flh expression domain is expanded toward the margin in zygotic oep mutants, occupying tiers 1-9. while flh is expressed ectopically at the margin. |
| Target Gene | flh |
| Upstream Gene | oep |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | The flh expression domain is expanded toward the margin in zygotic oep mutants, occupying tiers 1-9. while flh is expressed ectopically at the margin. |
| Target Gene | flh |
| Upstream Gene | oep |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | In ved-injected embryos, flh expression is strongly down-regulated, whereas in ved MO-injected embryos, flh expression domain is ectopically expanded in the dorsolateral marginal region. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | In ved-injected embryos, flh expression in the dorsolateral marginal region is strongly down-regulated. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | In ved-injected embryos, flh expression is strongly down-regulated, whereas in ved MO-injected embryos, flh expression domain is ectopically expanded in the dorsolateral marginal region. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | MASO |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | In ved-injected embryos, flh expression in the dorsolateral marginal region is strongly down-regulated. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | MASO |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Expression of organizer genes, gsc, flh, din, and cyc were strongly suppressed by vega2-HA mRNA injection |
| Target Gene | flh |
| Upstream Gene | vent |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Igor B. Dawid |
| Journal | genesis |
| Year | 2000 |
| Vol | 28 |
| Start Page | 58 |
| Evidence for Interaction | Expression of organizer genes, floating head (flh) was strongly suppressed by vega2-HA mRNA injection. |
| Target Gene | flh |
| Upstream Gene | vent |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Igor B. Dawid |
| Journal | genesis |
| Year | 2000 |
| Vol | 28 |
| Start Page | 58 |
| Evidence for Interaction | overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh. Injections of higher doses of cyc+ RNA result in expansion of the gsc and twhh domains as well. |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Dorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh. Injections of higher doses of cyc+ |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | The flh expression domain is expanded toward the margin in zygotic oep mutants, occupying tiers 1-9. while flh is expressed ectopically at the margin. |
| Target Gene | flh |
| Upstream Gene | oep |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | In ved-injected embryos, flh expression is strongly down-regulated, whereas in ved MO-injected embryos, flh expression domain is ectopically expanded in the dorsolateral marginal region. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | In ved-injected embryos, flh expression in the dorsolateral marginal region is strongly down-regulated. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | In ved-injected embryos, flh expression is strongly down-regulated, whereas in ved MO-injected embryos, flh expression domain is ectopically expanded in the dorsolateral marginal region. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | MASO |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | In ved-injected embryos, flh expression in the dorsolateral marginal region is strongly down-regulated. |
| Target Gene | flh |
| Upstream Gene | ved |
| Interaction Type | repress |
| Altered model | MASO |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Expression of organizer genes, gsc, flh, din, and cyc were strongly suppressed by vega2-HA mRNA injection |
| Target Gene | flh |
| Upstream Gene | vent |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Igor B. Dawid |
| Journal | genesis |
| Year | 2000 |
| Vol | 28 |
| Start Page | 58 |
| Evidence for Interaction | Expression of organizer genes, floating head (flh) was strongly suppressed by vega2-HA mRNA injection. |
| Target Gene | flh |
| Upstream Gene | vent |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Igor B. Dawid |
| Journal | genesis |
| Year | 2000 |
| Vol | 28 |
| Start Page | 58 |
| Evidence for Interaction | Ectopic expression of flh (100%, 15/15) was observed in bozmutant embryos following overexpression. |
| Target Gene | flh |
| Upstream Gene | eomes |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 5503 |
| Evidence for Interaction | Ectopic expression of gsc (85%, 17/20), chd (100%, 13/13) and flh (100%, 15/15) was observed in bozmutant embryos following overexpression, which showed that nwk/dhm gene function does not appear to be required for eomes to induce expression of organizer- |
| Target Gene | flh |
| Upstream Gene | eomes |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 5503 |
| Evidence for Interaction | Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh. Injections of higher doses of cyc+ RNA result in expansion of the gsc and twhh doma |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Dorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh. Injections of higher doses of cyc+ |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Dorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh Injections of higher doses of cyc+ R |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain , but not of gsc or twhh. Injections of higher doses of cyc+ RNA result in expansion of the gsc and twhh dom |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Expression analysis of the dorsal markers chd, gsc, flh and dharma at shield stage shows that they are expanded ventrally in wnt8 mutants as well as in vent;vox mutants. |
| Target Gene | flh |
| Upstream Gene | wnt8a |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Arne C. Lekven |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 3991 |
| Evidence for Interaction | At shield stage, expression of the dorsal marker floating head (flh) is expanded ventrally in wnt8 mutants. |
| Target Gene | flh |
| Upstream Gene | wnt8a |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Arne C. Lekven |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 3991 |
| Evidence for Interaction | Ectopic expression of flh (100%, 15/15) was observed in bozmutant embryos following overexpression. |
| Target Gene | flh |
| Upstream Gene | eomes |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 5503 |
| Evidence for Interaction | Ectopic expression of gsc (85%, 17/20), chd (100%, 13/13) and flh (100%, 15/15) was observed in bozmutant embryos following overexpression, which showed that nwk/dhm gene function does not appear to be required for eomes to induce expression of organizer- |
| Target Gene | flh |
| Upstream Gene | eomes |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 5503 |
| Evidence for Interaction | Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh. Injections of higher doses of cyc+ RNA result in expansion of the gsc and twhh doma |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Dorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh. Injections of higher doses of cyc+ |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Dorsal views at 50-60% epiboly. flh; gsc; twhh. Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain, but not of gsc or twhh Injections of higher doses of cyc+ R |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Control embryos showing expression of all marker genes in the shield. overexpression of 0.05 pg of cyc+ RNA results in expansion of the flh domain , but not of gsc or twhh. Injections of higher doses of cyc+ RNA result in expansion of the gsc and twhh dom |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Karuna Sampath |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 3331 |
| Evidence for Interaction | Expression analysis of the dorsal markers chd, gsc, flh and dharma at shield stage shows that they are expanded ventrally in wnt8 mutants as well as in vent;vox mutants. |
| Target Gene | flh |
| Upstream Gene | wnt8a |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Arne C. Lekven |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 3991 |
| Evidence for Interaction | At shield stage, expression of the dorsal marker floating head (flh) is expanded ventrally in wnt8 mutants. |
| Target Gene | flh |
| Upstream Gene | wnt8a |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | presumptive neural plate |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Arne C. Lekven |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 3991 |
| Evidence for Interaction | In homozygous bozm168 mutant embryos, the dorsal midline enexpression of flh is dramatically reduced. |
| Target Gene | flh |
| Upstream Gene | dharma |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Developmental Biology |
| Year | 1999 |
| Vol | 215 |
| Start Page | 190 |
| Evidence for Interaction | In wild-type embryos the expression of flh and twist is detected in dorsal mesendoderm cells that will later give rise to dorsal embryonic structures such as the notochord.In homozygous bozm168 mutant embryos, the dorsal midline enexpression of flh and tw |
| Target Gene | flh |
| Upstream Gene | dharma |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Robert K. Ho |
| Journal | Developmental Biology |
| Year | 1999 |
| Vol | 215 |
| Start Page | 190 |
| Evidence for Interaction | The expression of anterior mesendodermal markersis enlarged in injected embryos, while znot expression in the anterior notochordal domain is absent. |
| Target Gene | flh |
| Upstream Gene | nomo |
| Interaction Type | enhance |
| Altered model | blocking the production of protein |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Christian Haass |
| Journal | The EMBO Journal |
| Year | 2004 |
| Vol | 23 |
| Start Page | 3041 |
| Evidence for Interaction | The expression of anterior mesendodermal markers (gsc: presumptive hatching glandþprechordal plate; hgg1: presumptive hatching gland; hlx1: prechordal plate) is enlarged in injected embryos, while znot expression in the anterior notochordal domain is abse |
| Target Gene | flh |
| Upstream Gene | nomo |
| Interaction Type | enhance |
| Altered model | blocking the production of protein |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Christian Haass |
| Journal | The EMBO Journal |
| Year | 2004 |
| Vol | 23 |
| Start Page | 3041 |
| Evidence for Interaction | In addition, expression of otx5 was not detected in the flh-mutant epiphysis at 24s. |
| Target Gene | otx5 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | expression of otx5 was not detected in the flh-mutant epiphysis at 24-somite. |
| Target Gene | otx5 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | Expression of shh in ventral neuroectoderm, anterior floor plate and scattered posterior floor plate cells (arrowhead) in flh mutants is reduced. |
| Target Gene | shha |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | CNS |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Expression of shh in ventral neuroectoderm, anterior floor plate and scattered posterior floor plate cells in flh mutants. |
| Target Gene | shha |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | CNS |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | The posterior-most expression domain of myoD is drastically reduced in oep/ flh double mutants and absent in ntl mutants. |
| Target Gene | myod |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Note that this cell population is fused in flh mutant embryos and that myoD expression at this position is absent in ntl mutant and oep flh double mutant embryos. The posterior-most expression domain of myoD is also drastically reduced in oep flh double m |
| Target Gene | myod |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Expression of shh in ventral neuroectoderm, anterior floor plate and scattered posterior floor plate cells in flh mutants. |
| Target Gene | shha |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | Expression of shh in ventral neuroectoderm, anterior floor plate and scattered posterior floor plate cells (arrowhead) in flh mutants is reduced. |
| Target Gene | shha |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 14 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Wolfgang Driever |
| Journal | Development |
| Year | 1997 |
| Vol | 124 |
| Start Page | 327 |
| Evidence for Interaction | neuroD expression was absent in the flh-mutant epiphysis at both 14 and 24s, although one or two neuroD-positive cells were usually detected by 30 hpf |
| Target Gene | neurod |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | neuroD expression was absent in the flh-mutant epiphysis at both 14 and 24s, although one or two neuroD-positive cells were usually detected by 30 hpf |
| Target Gene | neurod |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | At 14s, a few ngn1-positive cells were detected in the wild-type epiphysis, whereas most flh mutants showed no expression. |
| Target Gene | neurog1 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | At 14s, a few ngn1-positive cells were detected in the wild-type epiphysis, whereas most flh mutants showed no expression. |
| Target Gene | neurog1 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | although flh expression is initiated normally in the flh mutant, it is greatly reduced by 24s and absent by 24 hours. |
| Target Gene | flh |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | although flh expression is initiated normally in the flh mutant, it is greatly reduced by 24s and absent by 24 hours. |
| Target Gene | flh |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 16 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | Expression of ash1a was initially normal in the flh-mutant epiphysis, but by 24s, the number of ash1a-expressing epiphysial cells was reduced to about 10 in mutants compared with 15- 20 in wild type. |
| Target Gene | ascl1a |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | neuroD expression was absent in the flh-mutant epiphysis at both 14 and 24s, although one or two neuroD-positive cells were usually detected by 30 hpf. |
| Target Gene | neurod |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | neuroD expression was absent in the flh-mutant epiphysis at both 14 and 24s, although one or two neuroD-positive cells were usually detected by 30 hpf |
| Target Gene | neurod |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | At 14s, a few ngn1-positive cells were detected in the wild-type epiphysis, whereas most flh mutants showed no expression. |
| Target Gene | neurog1 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | At 14s, a few ngn1-positive cells were detected in the wild-type epiphysis, whereas most flh mutants showed no expression. |
| Target Gene | neurog1 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | although flh expression is initiated normally in the flh mutant, it is greatly reduced by 24s and absent by 24 hours. |
| Target Gene | flh |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | although flh expression is initiated normally in the flh mutant, it is greatly reduced by 24s and absent by 24 hours. |
| Target Gene | flh |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 19 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | In flh mutants, the otx5 expression domain is reduced compared to WT. |
| Target Gene | otx5 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | diencephalon |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Jennifer O. Liang |
| Journal | nature genetics |
| Year | 2001 |
| Vol | 30 |
| Start Page | 117 |
| Evidence for Interaction | In flh mutants, the otx5 expression domain is reduced compared to WT. |
| Target Gene | otx5 |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | diencephalon |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Jennifer O. Liang |
| Journal | nature genetics |
| Year | 2001 |
| Vol | 30 |
| Start Page | 117 |
| Evidence for Interaction | At 24 hpf, the flh-mutant epiphysis contains around five to eight tbxCpositive cells whereas the wild-type epiphysis contains ~40 tbxC-positive cells. |
| Target Gene | tbx2b |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | At 24 hpf, the flh-mutant epiphysis contains around five to eight tbx2b positive cells whereas the wild-type epiphysis contains ~40 tbx2b-positive cells . |
| Target Gene | tbx2b |
| Upstream Gene | flh |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | epiphysis |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Stephen W. Wilson |
| Journal | Development |
| Year | 2003 |
| Vol | 130 |
| Start Page | 2455 |
| Evidence for Interaction | The flh expression domain is expanded toward the margin in zygotic oep mutants, occupying tiers 1-9. while flh is expressed ectopically at the margin. |
| Target Gene | flh |
| Upstream Gene | oep |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | Overexpression of cyclops and squint at different doses leads to the induction of floating head at low doses and the induction of both goosecoid (Gsc) and floating head (Flh) at higher doses |
| Target Gene | flh |
| Upstream Gene | ndr2 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Development |
| Year | 2000 |
| Vol | 127 |
| Start Page | 921 |
| Evidence for Interaction | 36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos. |
| Target Gene | flh |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 304 |
| Start Page | 525 |