| fgf3 | Stage | Hours | ZFIN expression range | marginal blastomere | germ ring | shield | neural plate | presumptive cephalic mesoderm | axial mesoderm | mesoderm | neuroectoderm | hindbrain neural plate | forebrain neural keel | presumptive rhombomere 4 | hindbrain neural keel | tail bud | tail | presumptive telencephalon | neural crest | somite | forebrain neural rod | midbrain hindbrain boundary neural rod | neural rod | pharyngeal endoderm | rhombomere 4 | brain | diencephalon | forebrain | hindbrain | midbrain | pharyngeal arch 3-7 skeleton | pharyngeal pouch 1 | otic vesicle | midbrain hindbrain boundary | pharyngeal pouch | pharyngeal pouch 2 | pharyngeal pouch 3 | retina | mandibular arch | unspecified |
| Zygote | 1-cell | 0 | ||||||||||||||||||||||||||||||||||||||
| Cleavage | 2-cell | 0.75 | ||||||||||||||||||||||||||||||||||||||
| 4-cell | 1.0 | |||||||||||||||||||||||||||||||||||||||
| 8-cell | 1.25 | |||||||||||||||||||||||||||||||||||||||
| 16-cell | 1.5 | |||||||||||||||||||||||||||||||||||||||
| 32-cell | 1.75 | |||||||||||||||||||||||||||||||||||||||
| 64-cell | 2.0 | |||||||||||||||||||||||||||||||||||||||
| Blastula | 124-cell | 2.25 | ||||||||||||||||||||||||||||||||||||||
| 256-cell | 2.5 | |||||||||||||||||||||||||||||||||||||||
| 512-cell | 2.75 | |||||||||||||||||||||||||||||||||||||||
| 1k-cell | 3.0 | |||||||||||||||||||||||||||||||||||||||
| High | 3.33 | |||||||||||||||||||||||||||||||||||||||
| Oblong | 3.66 | |||||||||||||||||||||||||||||||||||||||
| Sphere | 4.0 | |||||||||||||||||||||||||||||||||||||||
| Dome | 4.33 | I | I | |||||||||||||||||||||||||||||||||||||
| 30% epiboly | 4.66 | |||||||||||||||||||||||||||||||||||||||
| Gastrula | 50%-epiboly | 5.25 | ||||||||||||||||||||||||||||||||||||||
| Germ-ring | 5.66 | I | I | |||||||||||||||||||||||||||||||||||||
| Shield | 6.0 | |||||||||||||||||||||||||||||||||||||||
| 75% epiboly stage | 8.0 | |||||||||||||||||||||||||||||||||||||||
| 90% epiboly | 9.0 | |||||||||||||||||||||||||||||||||||||||
| bud stage | 10.0 | |||||||||||||||||||||||||||||||||||||||
| Segmentation | 1-4 somites | 10.33 | I | |||||||||||||||||||||||||||||||||||||
| 5-9 somites | 11.66 | |||||||||||||||||||||||||||||||||||||||
| 10-13 somites | 14.0 | ! | I | |||||||||||||||||||||||||||||||||||||
| 14-19 somites | 16.0 | ! | I | |||||||||||||||||||||||||||||||||||||
| 20-25 somites | 19.0 | I | I | |||||||||||||||||||||||||||||||||||||
| 26+ somites | 22.0 | I | I | I | I | I | I | |||||||||||||||||||||||||||||||||
| Pharyngula | Prim-5 | 24.0 | ||||||||||||||||||||||||||||||||||||||
| Prim-15 | 30.0 | I | I | |||||||||||||||||||||||||||||||||||||
| Prim-25 | 36.0 | |||||||||||||||||||||||||||||||||||||||
| High-pec | 42.0 | |||||||||||||||||||||||||||||||||||||||
| Hatching | Long-pec | 48.0 | ||||||||||||||||||||||||||||||||||||||
| Pec-fin | 60.0 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp4 expression at blastula stage. |
| Target Gene | bmp4 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp4 expression at blastula stage. |
| Target Gene | bmp4 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp4 expression at blastula stage. |
| Target Gene | bmp4 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp7 expression at blastula stage. |
| Target Gene | bmp7 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp7 expression at blastula stage. |
| Target Gene | bmp7 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp7 expression at blastula stage. |
| Target Gene | bmp7 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp7 expression at blastula stage. |
| Target Gene | bmp7 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | Microinjection of fgf3 leads to inhibition of bmp2b expression at blastula stage. |
| Target Gene | bmp2b |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bernard Thisse |
| Journal | Development |
| Year | 2004 |
| Vol | 131 |
| Start Page | 2853 |
| Evidence for Interaction | activation of the FGF/ERK pathway disrupts endoderm formation in the embryo and antagonizes the ability of an activated form of Tar/Acvr1b to induce endoderm at the animal pole. |
| Target Gene | acvr1b |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Thierry Lepage |
| Journal | Development |
| Year | 2006 |
| Vol | 133 |
| Start Page | 2189 |
| Evidence for Interaction | Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in the fgf3 injected embryo. |
| Target Gene | gata3 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | ectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo. |
| Target Gene | gata3 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | ectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in the fgf3 injected embryo. |
| Target Gene | gata3 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo. |
| Target Gene | gata3 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | chordino expression in control and fgf3 (5 pg)-injected embryos at 70% epiboly. Ectopic expression is induced in the entire epiblast, though it tends to be weaker than endogenous expression on the dorsal side. |
| Target Gene | chordin |
| Upstream Gene | fgf3 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | Ectopic expression of chd is induced in the entire epiblast, though it tends to be weaker than endogenous expression on the dorsal side in the fgf3 -injected embryos at 70% epiboly. |
| Target Gene | chordin |
| Upstream Gene | fgf3 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | fgf3 suppressed the anterior neural markers six3 and otx2 . |
| Target Gene | otx2 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | fgf3 suppressed the anterior neural markers six3. |
| Target Gene | six3a |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | fgf3 suppressed the anterior neural markers six3. |
| Target Gene | six3b |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | The expression of the posterior neuroectodermal marker hoxb1b was expanded, predominantly on the ventral side, by overexpression of any of the tested Fgf mRNAs. |
| Target Gene | hoxb1b |
| Upstream Gene | fgf3 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested fgf3 mRNAs. |
| Target Gene | hoxb1b |
| Upstream Gene | fgf3 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | When the same dose was applied, overexpression of fgf17b caused the expression domain of the anterior neural marker otx1 to shrink substantially. |
| Target Gene | otx1 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | overexpression of fgf8 or fgf3 reduced the otx1 domain size to a lesser degree but clearly decreased its expression level. |
| Target Gene | otx1 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | The expression of the posterior neuroectodermal marker hoxb1b was expanded, predominantly on the ventral side, by overexpression of any of the tested Fgf mRNAs. |
| Target Gene | hoxb1b |
| Upstream Gene | fgf3 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | The expression of the posterior neuroectodermal marker hoxb1b was expanded by overexpression of any of the tested fgf3 mRNAs. |
| Target Gene | hoxb1b |
| Upstream Gene | fgf3 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | When the same dose was applied, overexpression of fgf17b caused the expression domain of the anterior neural marker otx1 to shrink substantially. |
| Target Gene | otx1 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | overexpression of fgf8 or fgf3 reduced the otx1 domain size to a lesser degree but clearly decreased its expression level. |
| Target Gene | otx1 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Anming Meng |
| Journal | Developmental Biology |
| Year | 2004 |
| Vol | 271 |
| Start Page | 130 |
| Evidence for Interaction | Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo. |
| Target Gene | gata3 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | ectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | Gata3, normally expressed in the ventral epiblast fated to become nonneural epidermis, was completely abolished in fgf3 overexpressing embryo. |
| Target Gene | gata3 |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | fgf3 suppressed the anterior neural markers six3. |
| Target Gene | six3a |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | fgf3 suppressed the anterior neural markers six3. |
| Target Gene | six3b |
| Upstream Gene | fgf3 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hiroyuki Takeda |
| Journal | Developmental Biology |
| Year | 2002 |
| Vol | 244 |
| Start Page | 9 |
| Evidence for Interaction | fgf3 expression in the hindbrain of tailbud stage wild-type embryo, tailbud stage wnt8 morphant, tailbud stage Dfw8 homozygote. |
| Target Gene | fgf3 |
| Upstream Gene | wnt8a |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 1, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bruce B. Riley |
| Journal | Development |
| Year | 2003 |
| Vol | 131 |
| Start Page | 923 |
| Evidence for Interaction | fgf3 expression is reduced in the hindbrain of tailbud stage in wnt8 morphant. |
| Target Gene | fgf3 |
| Upstream Gene | wnt8a |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 1, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bruce B. Riley |
| Journal | Development |
| Year | 2003 |
| Vol | 131 |
| Start Page | 923 |
| Evidence for Interaction | fgf3 expression in the hindbrain of tailbud stage wild-type embryo, tailbud stage wnt8 morphant, tailbud stage Dfw8 homozygote. |
| Target Gene | fgf3 |
| Upstream Gene | wnt8a |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 1, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bruce B. Riley |
| Journal | Development |
| Year | 2003 |
| Vol | 131 |
| Start Page | 923 |
| Evidence for Interaction | fgf3 expression is reduced in the hindbrain of tailbud stage in wnt8 morphant. |
| Target Gene | fgf3 |
| Upstream Gene | wnt8a |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 10 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 1, 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Bruce B. Riley |
| Journal | Development |
| Year | 2003 |
| Vol | 131 |
| Start Page | 923 |
| Evidence for Interaction | 36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos. |
| Target Gene | fgf3 |
| Upstream Gene | oep |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Schier, A. F. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 304 |
| Start Page | 525 |