Expression Table

Gene Name: fgf24 (fibroblast growth factor 24)
Description: growth factor activity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

fgf24

Stage

Hours

ZFIN expression range

margin

anterior axial hypoblast

otic placode

polster

pectoral fin field

olfactory placode

gut

pharyngeal arch

intermediate cell mass of mesoderm

lens

blood

pectoral fin bud

retina

intestinal bulb

pectoral fin

apical ectodermal ridge pectoral fin

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Microinjection of fgf24 leads to inhibition of bmp7 expression at blastula stage.

Target Gene bmp7

Upstream Gene fgf24

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 4.66

Location of Interaction in evidence margin

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Microinjection of fgf24 leads to inhibition of bmp4 expression at blastula stage.

Target Gene bmp4

Upstream Gene fgf24

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 4.66

Location of Interaction in evidence margin

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Microinjection of fgf24 leads to inhibition of bmp2b, bmp4 and bmp7 expression at blastula stage.

Target Gene bmp4

Upstream Gene fgf24

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 4.66

Location of Interaction in evidence margin

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Microinjection of fgf24 leads to inhibition of bmp7 expression at blastula stage.

Target Gene bmp7

Upstream Gene fgf24

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 4.66

Location of Interaction in evidence YSL

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Microinjection of fgf24 leads to inhibition of bmp2b expression at blastula stage.

Target Gene bmp2b

Upstream Gene fgf24

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 4.66

Location of Interaction in evidence YSL

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction Microinjection of fgf24 leads to inhibition of bmp2b, bmp4 and bmp7 expression at blastula stage.

Target Gene bmp4

Upstream Gene fgf24

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 4.66

Location of Interaction in evidence YSL

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Bernard Thisse

Journal Development

Year 2004

Vol 131

Start Page 2853

Evidence for Interaction activation of the FGF/ERK pathway disrupts endoderm formation in the embryo and antagonizes the ability of an activated form of Tar/Acvr1b to induce endoderm at the animal pole.

Target Gene acvr1b

Upstream Gene fgf24

Interaction Type repress

Altered model overexpression

Time of Interaction in evidence 5.25

Location of Interaction in evidence mesendoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Thierry Lepage

Journal Development

Year 2006

Vol 133

Start Page 2189

Evidence for Interaction triple knockdown of fgf8, Fgf17b and fgf24 resulted in a large increase in the number of endodermal precursors that formed.

Target Gene sox17

Upstream Gene fgf24

Interaction Type repress

Altered model MASO

Time of Interaction in evidence 10

Location of Interaction in evidence endoderm

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Thierry Lepage

Journal Development

Year 2006

Vol 133

Start Page 2189

Evidence for Interaction	Microinjection of fgf24 leads to inhibition of bmp7 expression at blastula stage.
Target Gene	bmp7
Upstream Gene	fgf24
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	4.66
Location of Interaction in evidence	margin
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Bernard Thisse
Journal	Development
Year	2004
Vol	131
Start Page	2853

Evidence for Interaction	activation of the FGF/ERK pathway disrupts endoderm formation in the embryo and antagonizes the ability of an activated form of Tar/Acvr1b to induce endoderm at the animal pole.
Target Gene	acvr1b
Upstream Gene	fgf24
Interaction Type	repress
Altered model	overexpression
Time of Interaction in evidence	5.25
Location of Interaction in evidence	mesendoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Thierry Lepage
Journal	Development
Year	2006
Vol	133
Start Page	2189

Evidence for Interaction	triple knockdown of fgf8, Fgf17b and fgf24 resulted in a large increase in the number of endodermal precursors that formed.
Target Gene	sox17
Upstream Gene	fgf24
Interaction Type	repress
Altered model	MASO
Time of Interaction in evidence	10
Location of Interaction in evidence	endoderm
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Thierry Lepage
Journal	Development
Year	2006
Vol	133
Start Page	2189