Expression Table

Gene Name: epha4a
Description: receptor activity
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

epha4a

Stage

Hours

ZFIN expression range

axis

axial chorda mesoderm

mesoderm

hindbrain neural plate

forebrain neural keel

midbrain neural keel

neural keel

otic placode

segmental plate

tail bud

central nervous system

notochord

presumptive diencephalon

presumptive telencephalon

somite

neural tube

rhombomere

rhombomere 1

rhombomere 3

rhombomere 5

diencephalon

forebrain

hindbrain

midbrain

telencephalon

presumptive retina

forebrain midbrain boundary

cerebellum

spinal cord

tegmentum

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Rhombomeric expression of ephA4 is strongly affected and the prosencephalic domain shows massive posterior expansion.

Target Gene epha4a

Upstream Gene pou5f1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence rhombomeres

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2002

Vol 129

Start Page 917

Evidence for Interaction In val- embryos, ephB4a expression is induced and ephrin-B2a expression is repressed by Val in the caudal hindbrain.

Target Gene epha4a

Upstream Gene mafb

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence r5

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Nigel Holder

Journal Development

Year 2001

Vol 128

Start Page 571

Evidence for Interaction In val- embryos, ephB4a expression is induced and ephrin-B2a expression is repressed by Val in the caudal hindbrain.

Target Gene epha4a

Upstream Gene mafb

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence r6

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Nigel Holder

Journal Development

Year 2001

Vol 128

Start Page 571

Evidence for Interaction r5 expression of krx20 and r5/6 expression of ephB4a are downregulated in the val- embryo at the 4s stage.

Target Gene epha4a

Upstream Gene mafb

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence r5

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Nigel Holder

Journal Development

Year 2001

Vol 128

Start Page 571

Evidence for Interaction r5 expression of krx20 and r5/6 expression of ephB4a are downregulated in the val- embryo at the 4s stage.

Target Gene epha4a

Upstream Gene mafb

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence r6

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Nigel Holder

Journal Development

Year 2001

Vol 128

Start Page 571

Evidence for Interaction Wnt8a MO embryos showed dramatic reduction of ephA4/rtk1 expression in rhombomere 5.

Target Gene epha4a

Upstream Gene wnt8a

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 11.66

Location of Interaction in evidence brain

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Lilianna Solnica-Krezel

Journal Development

Year 2001

Vol 128

Start Page 3571

Evidence for Interaction Expression of ephB4a persists in r2 and 3rd arch neural crest in the val- embryo, but is absent from the caudal hindbrain at the 10-12s.

Target Gene epha4a

Upstream Gene mafb

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence r2

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Nigel Holder

Journal Development

Year 2001

Vol 128

Start Page 571

Evidence for Interaction Expression of ephB4a persists in r2 and 3rd arch neural crest in the val- embryo, but is absent from the caudal hindbrain at the 10-12s.

Target Gene epha4a

Upstream Gene mafb

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence r3

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Nigel Holder

Journal Development

Year 2001

Vol 128

Start Page 571

Evidence for Interaction	Rhombomeric expression of ephA4 is strongly affected and the prosencephalic domain shows massive posterior expansion.
Target Gene	epha4a
Upstream Gene	pou5f1
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	8
Location of Interaction in evidence	rhombomeres
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	2002
Vol	129
Start Page	917

Evidence for Interaction	In val- embryos, ephB4a expression is induced and ephrin-B2a expression is repressed by Val in the caudal hindbrain.
Target Gene	epha4a
Upstream Gene	mafb
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	r5
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Nigel Holder
Journal	Development
Year	2001
Vol	128
Start Page	571

Evidence for Interaction	r5 expression of krx20 and r5/6 expression of ephB4a are downregulated in the val- embryo at the 4s stage.
Target Gene	epha4a
Upstream Gene	mafb
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	r5
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Nigel Holder
Journal	Development
Year	2001
Vol	128
Start Page	571

Evidence for Interaction	Wnt8a MO embryos showed dramatic reduction of ephA4/rtk1 expression in rhombomere 5.
Target Gene	epha4a
Upstream Gene	wnt8a
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	11.66
Location of Interaction in evidence	brain
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Lilianna Solnica-Krezel
Journal	Development
Year	2001
Vol	128
Start Page	3571

Evidence for Interaction	Expression of ephB4a persists in r2 and 3rd arch neural crest in the val- embryo, but is absent from the caudal hindbrain at the 10-12s.
Target Gene	epha4a
Upstream Gene	mafb
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	11.66
Location of Interaction in evidence	r2
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Nigel Holder
Journal	Development
Year	2001
Vol	128
Start Page	571