Expression Table

Gene Name: eng2b (engrailed 2b )
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

eng2b

Stage

Hours

ZFIN expression range

midbrain hindbrain boundary neural plate

midbrain hindbrain boundary neural keel

neural keel

midbrain hindbrain boundary neural rod

midbrain

floor plate neural tube

midbrain hindbrain boundary

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction In spg embryos, en3 is downregulated in a similar fashion as en2.

Target Gene eng2b

Upstream Gene pou5f1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.25

Location of Interaction in evidence tectum

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 2002

Vol 129

Start Page 917

Evidence for Interaction Expressions of eng3 were expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.

Target Gene eng2b

Upstream Gene zfhx1

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Naomasa Miki

Journal Developmental Biology

Year 2000

Vol 228

Start Page 29

Evidence for Interaction Expressions of the neuroectodermal gene eng3 was expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.

Target Gene eng2b

Upstream Gene zfhx1

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 8

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Naomasa Miki

Journal Developmental Biology

Year 2000

Vol 228

Start Page 29

Evidence for Interaction eng3 expression normally starts at the 1-somite stage, and is never activated in noi -/ - mutants.

Target Gene eng2b

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction Eng3 expression normally starts at the 1-somite stage, and is never activated in noi -/ - mutants. From the 6-somite stage onwards, however, the expression narrows, persisting only in the dorsal part of the normal expression domain.

Target Gene eng2b

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10.33

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction Expressions of the neuroectodermal gene eng3 was expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.

Target Gene eng2b

Upstream Gene zfhx1

Interaction Type enhance

Altered model overexpression

Time of Interaction in evidence 10.33

Location of Interaction in evidence neuroectoderm

Evidence for Direct 2

Binding Site Sequence

Location of Site

Last Author Naomasa Miki

Journal Developmental Biology

Year 2000

Vol 228

Start Page 29

Evidence for Interaction Eng3 expression normally starts at the 1-somite stage, and is never activated in noi -/ - mutants. From the 6-somite stage onwards, however, the expression narrows, persisting only in the dorsal part of the normal expression domain.

Target Gene eng2b

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 11.66

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction These embryos also showed dramatic reduction of eng2b expression in rhombomere 5.

Target Gene eng2b

Upstream Gene wnt8a

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 11.66

Location of Interaction in evidence brain

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Lilianna Solnica-Krezel

Journal Development

Year 2001

Vol 128

Start Page 3571

Evidence for Interaction No eng3 is seen in null mutants at 22 hours, whereas in the strong allele noith44a a ventral eng3-positive spot can still be detected, and both ventral and dorsal expression is visible in weak noitb21 mutants.

Target Gene eng2b

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 22

Location of Interaction in evidence MHB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Michael Brand

Journal Development

Year 1998

Vol 125

Start Page 3049

Evidence for Interaction eng3 expression is lost in noi tu29a, but the posterior border of Pax6.1 expression is unperturbed and abuts progeny cells of mesencephalic precursors.

Target Gene eng2b

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 26

Location of Interaction in evidence FMB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Laure Bally-Cuif

Journal DEVELOPMENTAL DYNAMICS

Year 2003

Vol 227

Start Page 524

Evidence for Interaction Eng3 expression is lost in noi tu29a.

Target Gene eng2b

Upstream Gene pax2a

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 26

Location of Interaction in evidence FMB

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Laure Bally-Cuif

Journal DEVELOPMENTAL DYNAMICS

Year 2003

Vol 227

Start Page 524

Evidence for Interaction	In spg embryos, en3 is downregulated in a similar fashion as en2.
Target Gene	eng2b
Upstream Gene	pou5f1
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.25
Location of Interaction in evidence	tectum
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	2002
Vol	129
Start Page	917

Evidence for Interaction	Expressions of eng3 were expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.
Target Gene	eng2b
Upstream Gene	zfhx1
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	neuroectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Naomasa Miki
Journal	Developmental Biology
Year	2000
Vol	228
Start Page	29

Evidence for Interaction	Expressions of the neuroectodermal gene eng3 was expanded laterally and ventrally in the embryos injected with 50 pg of GFP-kheper RNA, compared to the uninjected control embryos.
Target Gene	eng2b
Upstream Gene	zfhx1
Interaction Type	enhance
Altered model	overexpression
Time of Interaction in evidence	8
Location of Interaction in evidence	neuroectoderm
Evidence for Direct	2
Binding Site Sequence
Location of Site
Last Author	Naomasa Miki
Journal	Developmental Biology
Year	2000
Vol	228
Start Page	29

Evidence for Interaction	eng3 expression normally starts at the 1-somite stage, and is never activated in noi -/ - mutants.
Target Gene	eng2b
Upstream Gene	pax2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10.33
Location of Interaction in evidence	MHB
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	1998
Vol	125
Start Page	3049

Evidence for Interaction	These embryos also showed dramatic reduction of eng2b expression in rhombomere 5.
Target Gene	eng2b
Upstream Gene	wnt8a
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	11.66
Location of Interaction in evidence	brain
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Lilianna Solnica-Krezel
Journal	Development
Year	2001
Vol	128
Start Page	3571

Evidence for Interaction	No eng3 is seen in null mutants at 22 hours, whereas in the strong allele noith44a a ventral eng3-positive spot can still be detected, and both ventral and dorsal expression is visible in weak noitb21 mutants.
Target Gene	eng2b
Upstream Gene	pax2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	22
Location of Interaction in evidence	MHB
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Michael Brand
Journal	Development
Year	1998
Vol	125
Start Page	3049

Evidence for Interaction	eng3 expression is lost in noi tu29a, but the posterior border of Pax6.1 expression is unperturbed and abuts progeny cells of mesencephalic precursors.
Target Gene	eng2b
Upstream Gene	pax2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	26
Location of Interaction in evidence	FMB
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Laure Bally-Cuif
Journal	DEVELOPMENTAL DYNAMICS
Year	2003
Vol	227
Start Page	524

Evidence for Interaction	Eng3 expression is lost in noi tu29a.
Target Gene	eng2b
Upstream Gene	pax2a
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	26
Location of Interaction in evidence	FMB
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Laure Bally-Cuif
Journal	DEVELOPMENTAL DYNAMICS
Year	2003
Vol	227
Start Page	524