Expression Table

Gene Name: eng2a (engrailed 2a )
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007
eng2aStageHoursZFIN expression rangeneural platemidbrain hindbrain boundary neural platemidbrain neural keelmidbrain hindbrain boundary neural keelmidbrain hindbrain boundary neural rodmuscle pioneerslow muscle cellfloor plate neural tubemidbrain hindbrain boundarymandibular musclewhole organismunspecified
Zygote1-cell0             
Cleavage2-cell0.75             
4-cell1.0             
8-cell1.25             
16-cell1.5             
32-cell1.75             
64-cell2.0             
Blastula124-cell2.25I           I
256-cell2.5I           I
512-cell2.75I           I
1k-cell3.0I           I
High3.33I           I
Oblong3.66I           I
Sphere4.0I           I
Dome4.33I           I
30% epiboly4.66I           I
Gastrula50%-epiboly5.25I          XI
Germ-ring5.66I          XI
Shield6.0           ? 
75% epiboly stage8.0I          ?I
90% epiboly9.0           ?I
bud stage10.0           ? 
Segmentation1-4 somites10.33             
5-9 somites11.66             
10-13 somites14.0      I      
14-19 somites16.0             
20-25 somites19.0             
26+ somites22.0            I
PharyngulaPrim-524.0             
Prim-1530.0             
Prim-2536.0            I
High-pec42.0            I
HatchingLong-pec48.0             
Pec-fin60.0I        I   


Literature Evidence



Evidence for InteractionEng2 is expressed from 90% epiboly onwards in the wild type, and expression is detectable at a strongly reduced level in noi -/- mutants, in a subpopulation of the wild-type domain that fades away with the appearance of the first somite.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemidbrain
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionEng2 is expressed from 90% epiboly onwards in the wild type, and expression is detectable at a strongly reduced level in noi -/- mutants, in a subpopulation of the wild-type domain that fades away with the appearance of the first somite.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidencemidbrain
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionEng2 is expressed from 90% epiboly onwards in the wild type, and expression is detectable at a strongly reduced level in noi -/- mutants, in a subpopulation of the wild-type domain that fades away with the appearance of the first somite.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidenceMHB
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionEng2 is expressed from 90% epiboly onwards in the wild type, and expression is detectable at a strongly reduced level in noi -/- mutants, in a subpopulation of the wild-type domain that fades away with the appearance of the first somite.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence9
Location of Interaction in evidenceMHB
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionIn spg mutant embryos, en2 is downregulated from its initiation of expression.
Target Geneeng2a
Upstream Genepou5f1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence10
Location of Interaction in evidenceneuroectoderm
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year2002
Vol129
Start Page917



Evidence for Interactionen2 expression is reduced in wnt8b MO. At 7 somites, en2 is expressed in the MHB and midbrain. Expression is substantially reduced in both wnt8b and nlk morphants.
Target Geneeng2a
Upstream Genewnt8b
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10
Location of Interaction in evidencebrain
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorRandall T. Moon
JournalDevelopment
Year2004
Vol131
Start Page2899



Evidence for InteractionAt 7 somites, en2 expression in the MHB and midbrain is reduced in wnt8b MO.
Target Geneeng2a
Upstream Genewnt8b
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10
Location of Interaction in evidencebrain
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorRandall T. Moon
JournalDevelopment
Year2004
Vol131
Start Page2899



Evidence for InteractionThe eng2 expression at the prospective MHB was abrogated in the Gbx overexpress embryo. MO-gbx2 down-regulation of eng2 (40%, n= 28 ) was observed in some of the embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence10.33
Location of Interaction in evidenceforebrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionDownregulation of wnt1 and eng2 was observed in MO-gbx2 injected embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10.33
Location of Interaction in evidenceforebrain
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionDownregulation of wnt1 and eng2 was observed in MO-gbx2 injected embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10.33
Location of Interaction in evidenceforebrain
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionThe eng2 expression at the prospective MHB was abrogated in the Gbx overexpress embryo. MO-gbx2 down-regulation of eng2 (40%, n= 28 ) was observed in some of the embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence10.33
Location of Interaction in evidencemidbrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionDownregulation of wnt1 and eng2 was observed in MO-gbx2 injected embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10.33
Location of Interaction in evidencemidbrain
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionDownregulation of wnt1 and eng2 was observed in MO-gbx2 injected embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10.33
Location of Interaction in evidencemidbrain
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionThe eng2 expression at the prospective MHB was abrogated in the Gbx overexpress embryo. MO-gbx2 down-regulation of eng2 (40%, n= 28 ) was observed in some of the embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modeloverexpression
Time of Interaction in evidence10.33
Location of Interaction in evidenceMHB
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionDownregulation of wnt1 and eng2 was observed in MO-gbx2 injected embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10.33
Location of Interaction in evidenceMHB
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionDownregulation of wnt1 and eng2 was observed in MO-gbx2 injected embryos.
Target Geneeng2a
Upstream Genegbx2
Interaction Typeenhance
Altered modelMASO
Time of Interaction in evidence10.33
Location of Interaction in evidenceMHB
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for Interactioneng2 expression is detectable at a strongly reduced level in noi -/- mutants.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence11.66
Location of Interaction in evidencemidbrain
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionIn spgm793 mutants, initial eng2 expression is detectable only at reduced levels and in smaller domains corresponding to the reduced pax2.1 domains.
Target Geneeng2a
Upstream Genepou5f1
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence11.66
Location of Interaction in evidencemidbrain
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorWolfgang Driever
JournalDevelopment
Year2001
Vol128
Start Page4165



Evidence for Interactioneng2 expression is detectable at a strongly reduced level in noi -/- mutants.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered modelmutant embryos
Time of Interaction in evidence11.66
Location of Interaction in evidenceMHB
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence19
Location of Interaction in evidenceforebrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence19
Location of Interaction in evidenceforebrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence19
Location of Interaction in evidencemidbrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence19
Location of Interaction in evidencemidbrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence19
Location of Interaction in evidenceMHB
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence19
Location of Interaction in evidenceMHB
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence24
Location of Interaction in evidencemidbrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence24
Location of Interaction in evidencemidbrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence24
Location of Interaction in evidenceMHB
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence24
Location of Interaction in evidenceMHB
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionEng2 expression in the neural tube is absent in noitu29a; lateral expression in presumptive eye muscles is not affected. In the weak noity31a allele ventral expression is seen, and is located, similar to the eng3 expression, in the ventral neural tube.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered model 
Time of Interaction in evidence24
Location of Interaction in evidenceMHB
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionEng2 expression in the neural tube is absent in noitu29a; lateral expression in presumptive eye muscles is not affected. In the weak noity31a allele ventral expression is seen, and is located, similar to the eng3 expression, in the ventral neural tube.
Target Geneeng2a
Upstream Genepax2a
Interaction Typeenhance
Altered model 
Time of Interaction in evidence24
Location of Interaction in evidenceMHB
Evidence for Direct1
Binding Site Sequence 
Location of Site 
Last AuthorMichael Brand
JournalDevelopment
Year1998
Vol125
Start Page3049



Evidence for InteractionExpression of eng2 alongbthe A-P axis is enhanced at 24 hpf in Hs6st morphants.
Target Geneeng2a
Upstream Genehs6st2
Interaction Typerepress
Altered modelMASO
Time of Interaction in evidence24
Location of Interaction in evidencesomites
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorDanica Zivkovic
JournalTHE JOURNAL OF BIOLOGICAL CHEMISTRY
Year2003
Vol278
Start Page31118



Evidence for InteractionExpression of eng2 alongbthe A-P axis is enhanced at 24 hpf in Hs6st morphants.
Target Geneeng2a
Upstream Genehs6st2
Interaction Typerepress
Altered modelMASO
Time of Interaction in evidence24
Location of Interaction in evidencesomites
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorDanica Zivkovic
JournalTHE JOURNAL OF BIOLOGICAL CHEMISTRY
Year2003
Vol278
Start Page31118



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence28
Location of Interaction in evidencemidbrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence28
Location of Interaction in evidencemidbrain
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence28
Location of Interaction in evidenceMHB
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionIn the embryos injected gbx2 mRNA data, eng2a was suppressed.
Target Geneeng2a
Upstream Genegbx2
Interaction Typerepress
Altered modeloverexpression
Time of Interaction in evidence28
Location of Interaction in evidenceMHB
Evidence for Direct2
Binding Site Sequence 
Location of Site 
Last AuthorKyo Yamasu
JournalDevelopmental Dynamics
Year2003
Vol228
Start Page433



Evidence for InteractionExpression of eng2 along the A-P axis is enhanced at 48 hpf in Hs6st morphants.
Target Geneeng2a
Upstream Genehs6st2
Interaction Typerepress
Altered modelMASO
Time of Interaction in evidence48
Location of Interaction in evidencesomites
Evidence for Direct3
Binding Site Sequence 
Location of Site 
Last AuthorDanica Zivkovic
JournalTHE JOURNAL OF BIOLOGICAL CHEMISTRY
Year2003
Vol278
Start Page31118