Expression Table

Gene Name: emx1 (empty spiracles homeobox 1 )
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

emx1

Stage

Hours

ZFIN expression range

neural plate

head

presumptive telencephalon

pronephric duct

epiphysis

forebrain

telencephalon

pronephric duct opening

dorsal telencephalon

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

X-I

8-cell

1.25

X-I

16-cell

1.5

X-I

32-cell

1.75

X-I

64-cell

2.0

X-I

Blastula

124-cell

2.25

X-I

256-cell

2.5

X-I

512-cell

2.75

X-I

1k-cell

3.0

X-I

High

3.33

X-I

Oblong

3.66

X-I

Sphere

4.0

X-I

Dome

4.33

X-I

30% epiboly

4.66

X-I

Gastrula

50%-epiboly

5.25

X-I

Germ-ring

5.66

X-I

Shield

6.0

75% epiboly stage

8.0

X-I

90% epiboly

9.0

X-I

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction The expression of emx1 is detected in the developing forebrain region in wild-type embryos and absent in severely affected boz mutant embryos.

Target Gene emx1

Upstream Gene dharma

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence forebrain

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Biology

Year 1999

Vol 215

Start Page 190

Evidence for Interaction	The expression of emx1 is detected in the developing forebrain region in wild-type embryos and absent in severely affected boz mutant embryos.
Target Gene	emx1
Upstream Gene	dharma
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	forebrain
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Biology
Year	1999
Vol	215
Start Page	190