Expression Table

Gene Name: drl (draculin)
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

drl

Stage

Hours

ZFIN expression range

forerunner cell group

anterior axial hypoblast

ventral mesoderm

lateral mesoderm

lateral plate mesoderm

hematopoietic system

anterior lateral mesoderm

posterior lateral mesoderm

rostral blood island

macrophage

intermediate cell mass of mesoderm

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction dra is reduced in tbx16 mutant at 70% epiboly stage.

Target Gene drl

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence mesendoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction drl expression domain absent at Bud through 12s in the tbx16 mutant.

Target Gene drl

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 8

Location of Interaction in evidence paraxil mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction dra is absent in tbx16 mutant at Bud stage

Target Gene drl

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 10

Location of Interaction in evidence paraxil mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction Expression of drl was lost in most of the PLM by 10 somites (14 hpf) in tal1 MO-injected embryos.

Target Gene drl

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 14

Location of Interaction in evidence blood

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction drl expression was lost in most of the PLM by 10 somites in MO-injected embryos.

Target Gene drl

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 14

Location of Interaction in evidence blood precursors

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction drl expression domain absent at Bud through 12s in the tbx16 mutant.

Target Gene drl

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence paraxil mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction dra is absent in tbx16 mutant at 12 somite stage.

Target Gene drl

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence paraxil mesoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction drl expression domain absent at Bud through 12s in the tbx16 mutant.

Target Gene drl

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction dra is absent in tbx16 mutant at 12 somite stage.

Target Gene drl

Upstream Gene tbx16

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 14

Location of Interaction in evidence tailbud

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Robert K. Ho

Journal Developmental Cell

Year 2004

Vol 7

Start Page 251

Evidence for Interaction	dra is reduced in tbx16 mutant at 70% epiboly stage.
Target Gene	drl
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	8
Location of Interaction in evidence	mesendoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	drl expression domain absent at Bud through 12s in the tbx16 mutant.
Target Gene	drl
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	8
Location of Interaction in evidence	paraxil mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	dra is absent in tbx16 mutant at Bud stage
Target Gene	drl
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	10
Location of Interaction in evidence	paraxil mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251

Evidence for Interaction	Expression of drl was lost in most of the PLM by 10 somites (14 hpf) in tal1 MO-injected embryos.
Target Gene	drl
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	14
Location of Interaction in evidence	blood
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	drl expression was lost in most of the PLM by 10 somites in MO-injected embryos.
Target Gene	drl
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	14
Location of Interaction in evidence	blood precursors
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9

Evidence for Interaction	dra is absent in tbx16 mutant at 12 somite stage.
Target Gene	drl
Upstream Gene	tbx16
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	14
Location of Interaction in evidence	paraxil mesoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Robert K. Ho
Journal	Developmental Cell
Year	2004
Vol	7
Start Page	251