Expression Table

Gene Name: dld (delta D)
Description: calcium ion binding, Notch signaling pathway
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

dld	Stage	Hours	ZFIN expression range	presumptive paraxial mesoderm	ectoderm	otic placode	segmental plate	tail bud	somite	hindbrain	unspecified
Zygote	1-cell	0
Cleavage	2-cell	0.75
	4-cell	1.0
	8-cell	1.25
	16-cell	1.5
	32-cell	1.75
	64-cell	2.0
Blastula	124-cell	2.25
	256-cell	2.5
	512-cell	2.75
	1k-cell	3.0
	High	3.33
	Oblong	3.66
	Sphere	4.0
	Dome	4.33
	30% epiboly	4.66
Gastrula	50%-epiboly	5.25									I
	Germ-ring	5.66	I	I							I
	Shield	6.0
	75% epiboly stage	8.0
	90% epiboly	9.0
	bud stage	10.0			X
Segmentation	1-4 somites	10.33			X		I
	5-9 somites	11.66					I
	10-13 somites	14.0
	14-19 somites	16.0
	20-25 somites	19.0
	26+ somites	22.0	I								I
Pharyngula	Prim-5	24.0
	Prim-15	30.0
	Prim-25	36.0
	High-pec	42.0
Hatching	Long-pec	48.0
Hatching	Pec-fin	60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Expression of all three Delta genes was affected in the ash1a morphants and more severely reduced or completely absent in the ash1a/ngn1-double morphants.

Target Gene dld

Upstream Gene ascl1a

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 19

Location of Interaction in evidence epiphysis

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Stephen W. Wilson

Journal Development

Year 2003

Vol 130

Start Page 2455

Evidence for Interaction	Expression of all three Delta genes was affected in the ash1a morphants and more severely reduced or completely absent in the ash1a/ngn1-double morphants.
Target Gene	dld
Upstream Gene	ascl1a
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	19
Location of Interaction in evidence	epiphysis
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Stephen W. Wilson
Journal	Development
Year	2003
Vol	130
Start Page	2455