Expression Table

Gene Name: cmyb (transcription factor cmyb )
Description: DNA binding
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

cmyb

Stage

Hours

ZFIN expression range

YSL

ventral mesoderm

lateral mesoderm

integument

anterior lateral plate mesoderm

hematopoietic system

intermediate cell mass of mesoderm

dorsal aorta

ventral wall of dorsal aorta

retina

hematopoietic stem cell

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

?-I

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction Expression of c-myb at 26 hpf in HSCs was almost entirely lost in tal1 morphant embryos.

Target Gene cmyb

Upstream Gene tal1

Interaction Type enhance

Altered model MASO

Time of Interaction in evidence 26

Location of Interaction in evidence hematopoietic stem cells

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Roger Patient

Journal blood

Year 2005

Vol 105

Start Page 9

Evidence for Interaction	Expression of c-myb at 26 hpf in HSCs was almost entirely lost in tal1 morphant embryos.
Target Gene	cmyb
Upstream Gene	tal1
Interaction Type	enhance
Altered model	MASO
Time of Interaction in evidence	26
Location of Interaction in evidence	hematopoietic stem cells
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Roger Patient
Journal	blood
Year	2005
Vol	105
Start Page	9