Expression Table

Gene Name: chd (chordin)
Description: negative regulation of BMP signaling pathway
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

chd

Stage

Hours

ZFIN expression range

blastodisc

presumptive dorsal mesoderm

presumptive ectoderm

presumptive mesoderm

presumptive neural plate

presumptive shield

blastoderm

margin

axis

shield

axial chorda mesoderm

axial hypoblast

dorsal axial hypoblast

neural plate

axial mesoderm

mesoderm

prechordal plate

ectoderm

neuroectoderm

forebrain neural keel

midbrain neural keel

presumptive rhombomere 2

presumptive rhombomere 4

adaxial cell

tail bud

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction 36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos.

Target Gene chd

Upstream Gene oep

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.25

Location of Interaction in evidence margin

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Schier, A. F.

Journal Dev Biol

Year 2007

Vol 304

Start Page 525

Evidence for Interaction	36 genes that are expressed throughout the margin in wild-type embryos, but lack marginal expression in MZoep embryos.
Target Gene	chd
Upstream Gene	oep
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.25
Location of Interaction in evidence	margin
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Schier, A. F.
Journal	Dev Biol
Year	2007
Vol	304
Start Page	525