Expression Table

Gene Name: bhik (bhikhari , also known as cb160, bik )
Description: molecular function unknown
Key: I = Interpolated; X = Not Detected; ? = Inconsistent data; ! = Expressed but no region
Table built from ZFIN data (http://zfin.org/zf_info/downloads.html): Tue Dec 4 14:01:03 PST 2007

bhik

Stage

Hours

ZFIN expression range

axis

anterior axial hypoblast

axial chorda mesoderm

presumptive segmental plate

mesoderm

ventral mesoderm

polster

segmental plate

tail bud

lateral plate mesoderm

somite

whole organism

unspecified

Zygote

1-cell

Cleavage

2-cell

0.75

4-cell

1.0

8-cell

1.25

16-cell

1.5

32-cell

1.75

64-cell

2.0

Blastula

124-cell

2.25

256-cell

2.5

512-cell

2.75

1k-cell

3.0

High

3.33

Oblong

3.66

Sphere

4.0

Dome

4.33

30% epiboly

4.66

Gastrula

50%-epiboly

5.25

Germ-ring

5.66

Shield

6.0

75% epiboly stage

8.0

90% epiboly

9.0

bud stage

10.0

Segmentation

1-4 somites

10.33

5-9 somites

11.66

10-13 somites

14.0

14-19 somites

16.0

20-25 somites

19.0

26+ somites

22.0

Pharyngula

Prim-5

24.0

Prim-15

30.0

Prim-25

36.0

High-pec

42.0

Hatching

Long-pec

48.0

Pec-fin

60.0

Literature Evidence

1. Mutant embryo and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
2. Overexpression of specific genes and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
3. Morpholino knock-down the expression of specific gene and in-situ hybridization (or Q-PCR) showed the downstream target genes are affected
4. The interaction sites of the input to downstream target genes have been identified by isolated the module and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
5. The interaction sites of the input to downstream target genes have been identified by coinject the Morpholino or over-expression the genes for reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)
6. The interaction sites of the input to downstream target genes have been identified by mutation the binding sites and reporter assay (CAT, GFP, beta-galactosidase, leciferase or Q-PCR assay etc.)

Evidence for Interaction bik is induced in bon and MZsur;bon and MZsur;bon/+ but not in foxh1 mutant embryos.

Target Gene bhik

Upstream Gene foxh1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.25

Location of Interaction in evidence mesendoderm

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Schier, A. F.

Journal Development

Year 2003

Vol 130

Start Page 5589

Evidence for Interaction bik is not in MZsur mutant (foxh1) embryos.

Target Gene bhik

Upstream Gene foxh1

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.66

Location of Interaction in evidence axis

Evidence for Direct 1

Binding Site Sequence

Location of Site

Last Author Schier, A. F.

Journal Development

Year 2003

Vol 130

Start Page 5589

Evidence for Interaction 27 genes that are expressed in the dorsal margin in wild type, but lack margin expression in MZoep mutants.

Target Gene bhik

Upstream Gene oep

Interaction Type enhance

Altered model mutant embryos

Time of Interaction in evidence 5.25

Location of Interaction in evidence blastomere

Evidence for Direct 3

Binding Site Sequence

Location of Site

Last Author Schier, A. F.

Journal Dev Biol

Year 2007

Vol 304

Start Page 525

Evidence for Interaction	bik is induced in bon and MZsur;bon and MZsur;bon/+ but not in foxh1 mutant embryos.
Target Gene	bhik
Upstream Gene	foxh1
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.25
Location of Interaction in evidence	mesendoderm
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Schier, A. F.
Journal	Development
Year	2003
Vol	130
Start Page	5589

Evidence for Interaction	bik is not in MZsur mutant (foxh1) embryos.
Target Gene	bhik
Upstream Gene	foxh1
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.66
Location of Interaction in evidence	axis
Evidence for Direct	1
Binding Site Sequence
Location of Site
Last Author	Schier, A. F.
Journal	Development
Year	2003
Vol	130
Start Page	5589

Evidence for Interaction	27 genes that are expressed in the dorsal margin in wild type, but lack margin expression in MZoep mutants.
Target Gene	bhik
Upstream Gene	oep
Interaction Type	enhance
Altered model	mutant embryos
Time of Interaction in evidence	5.25
Location of Interaction in evidence	blastomere
Evidence for Direct	3
Binding Site Sequence
Location of Site
Last Author	Schier, A. F.
Journal	Dev Biol
Year	2007
Vol	304
Start Page	525