| alk8 | Stage | Hours | ZFIN expression range | eye | brain | whole organism | unspecified |
| Zygote | 1-cell | 0 | |||||
| Cleavage | 2-cell | 0.75 | |||||
| 4-cell | 1.0 | ||||||
| 8-cell | 1.25 | ||||||
| 16-cell | 1.5 | ||||||
| 32-cell | 1.75 | ||||||
| 64-cell | 2.0 | ||||||
| Blastula | 124-cell | 2.25 | |||||
| 256-cell | 2.5 | ||||||
| 512-cell | 2.75 | ||||||
| 1k-cell | 3.0 | ||||||
| High | 3.33 | ||||||
| Oblong | 3.66 | ||||||
| Sphere | 4.0 | ||||||
| Dome | 4.33 | ||||||
| 30% epiboly | 4.66 | ||||||
| Gastrula | 50%-epiboly | 5.25 | |||||
| Germ-ring | 5.66 | ||||||
| Shield | 6.0 | ||||||
| 75% epiboly stage | 8.0 | ||||||
| 90% epiboly | 9.0 | ||||||
| bud stage | 10.0 | ||||||
| Segmentation | 1-4 somites | 10.33 | |||||
| 5-9 somites | 11.66 | ||||||
| 10-13 somites | 14.0 | ||||||
| 14-19 somites | 16.0 | ||||||
| 20-25 somites | 19.0 | ||||||
| 26+ somites | 22.0 | ||||||
| Pharyngula | Prim-5 | 24.0 | |||||
| Prim-15 | 30.0 | ||||||
| Prim-25 | 36.0 | ||||||
| High-pec | 42.0 | ||||||
| Hatching | Long-pec | 48.0 | |||||
| Pec-fin | 60.0 |
| Evidence for Interaction | injected mRNA encoding a constitutively active version of Alk8 can rescue the strong dorsalization of bmp2b/swirl mutants. |
| Target Gene | bmp2b |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hammerschmidt, M. |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 849 |
| Evidence for Interaction | injected mRNA encoding a constitutively active version of Alk8 can rescue the strong dorsalization of bmp7/snailhouse mutants |
| Target Gene | bmp7 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hammerschmidt, M. |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 849 |
| Evidence for Interaction | the data suggest that Alk8 acts as a Bmp2b/7 receptor upstream of Smad5. |
| Target Gene | smad5 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hammerschmidt, M. |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 849 |
| Evidence for Interaction | the data suggest that Alk8 acts as a Bmp2b/7 receptor upstream of Smad5. |
| Target Gene | smad5 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hammerschmidt, M. |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 849 |
| Evidence for Interaction | Ved expression is down-regulated at late blastula stage and up-regulated during gastrulation in BMP signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | EVL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Ved expression is down-regulated at late blastula stage in MZ-laf/bmpRI(alk8) mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | EVL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | in alk8 (a type I TGFbeta receptor) MO-injected embryos, vhnf1 expression in the endoderm is significantly reduced. |
| Target Gene | hnf1b |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Lin, S. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 303 |
| Start Page | 561 |
| Evidence for Interaction | vhnf1 acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish. |
| Target Gene | hnf1b |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Lin, S. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 303 |
| Start Page | 561 |
| Evidence for Interaction | Inhibiting both maternally and zygotically supplied alk8 gene products with morpholino antisense oligonucleotides leads to much stronger dorsalization, can be rescued by injected mRNA encoding Alk8 or the Bmp-regulated transcription factor Smad5. |
| Target Gene | smad5 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hammerschmidt, M. |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 849 |
| Evidence for Interaction | Inhibiting both maternally and zygotically supplied alk8 gene products with morpholino antisense oligonucleotides leads to much stronger dorsalization, can be rescued by injected mRNA encoding Alk8 or the Bmp-regulated transcription factor Smad5. |
| Target Gene | smad5 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 1, 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Hammerschmidt, M. |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 849 |
| Evidence for Interaction | ved transcripts are ventrally restricted by BMP factors such as bmp2b, bmp7, smad5, and alk8, and by dorsal factors (chd and gsc) |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | overexpression |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 2 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Cotelli, F. |
| Journal | Dev Dyn |
| Year | 2004 |
| Vol | 3 |
| Start Page | 494 |
| Evidence for Interaction | Laf/Alk8 as a type I BMP receptor required for the specification of ventral cell fates. |
| Target Gene | alk8 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 4.66 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Ved expression is down-regulated at late blastula stage and up-regulated during gastrulation in BMP signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | EVL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI(alk8) signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 5.25 |
| Location of Interaction in evidence | EVL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | We never found a homozygous laftm110b embryo that had a wild-type or ventralized phenotype, consistent with the Laf/Alk8 receptor functioning downstream of the Bmp2b. |
| Target Gene | alk8 |
| Upstream Gene | bmp2b |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 6 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | In most MZ-laf/alk8 mutants dlx3 expression is absent. |
| Target Gene | dlx3b |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | In most MZ-laf/alk8 mutants dlx3 expression is absent, while in the remaining embryos it is severely reduced. |
| Target Gene | dlx3b |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Foxb1.2 expression is expanded ventrally in MZ-laf embryos at 70% epiboly, the expansion is more pronounced in more vegetal regions. |
| Target Gene | foxb1.2 |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | In MZ-laf/alk8 mutant embryos gata2 expression is absent. |
| Target Gene | gata2 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | In MZ-laf/alk8 mutant embryos gata2 expression is absent. |
| Target Gene | gata2 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Expression of foxb1.2 in wild-type and MZ-laf embryos at 70% epiboly. foxb1.2 expression is expanded ventrally, the expansion is more pronounced in more vegetal regions. |
| Target Gene | foxb1.2 |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | mesendoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Foxb1.2 expression is expanded ventrally in MZ-laf embryos at 70% epiboly, the expansion is more pronounced in more vegetal regions. |
| Target Gene | foxb1.2 |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Foxb1.2 expression is expanded ventrally in MZ-laf embryos at 70% epiboly, the expansion is more pronounced in more vegetal regions. |
| Target Gene | foxb1.2 |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | neuroectoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Foxb1.2 expression is expanded ventrally in MZ-laf embryos at 70% epiboly, the expansion is more pronounced in more vegetal regions. |
| Target Gene | foxb1.2 |
| Upstream Gene | alk8 |
| Interaction Type | repress |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | presumptive brain |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 8 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | epiblast |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | margin |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | We never found a homozygous laftm110b embryo that had a wild-type or ventralized phenotype , consistent with the Laf/Alk8 receptor functioning downstream of the Bmp2b and Bmp7 ligands. |
| Target Gene | alk8 |
| Upstream Gene | bmp7 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | ventral mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Mary C. Mullins |
| Journal | Development |
| Year | 2001 |
| Vol | 128 |
| Start Page | 859 |
| Evidence for Interaction | Ved expression is up-regulated during gastrulation in MZ-laf/bmpRI signaling mutants. |
| Target Gene | ved |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 9 |
| Location of Interaction in evidence | YSL |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Franco Cotelli |
| Journal | Developmental Dynamics |
| Year | 2004 |
| Vol | 230 |
| Start Page | 494 |
| Evidence for Interaction | Bmp receptor lost-a-fin/alk8 is required for rostral pu.1 expression and myelopoiesis, Furthermore, the role of Alk8 in myelopoiesis was genetically separable from its earlier role in dorsal-ventral embryonic patterning. |
| Target Gene | spi1 |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | mutant embryos |
| Time of Interaction in evidence | 11.66 |
| Location of Interaction in evidence | lateral plate mesoderm |
| Evidence for Direct | 1 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Lieschke, G. J. |
| Journal | Curr Biol |
| Year | 2006 |
| Vol | 16 |
| Start Page | 506 |
| Evidence for Interaction | hnf1b acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish. |
| Target Gene | hnf1b |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | gut |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Lin, S. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 303 |
| Start Page | 561 |
| Evidence for Interaction | hnf1b acts downstream of the signaling pathways of RA, Bmp, and Fgf to regulate pancreas development in zebrafish. |
| Target Gene | hnf1b |
| Upstream Gene | alk8 |
| Interaction Type | enhance |
| Altered model | MASO |
| Time of Interaction in evidence | 24 |
| Location of Interaction in evidence | pronephros |
| Evidence for Direct | 3 |
| Binding Site Sequence | |
| Location of Site | |
| Last Author | Lin, S. |
| Journal | Dev Biol |
| Year | 2007 |
| Vol | 303 |
| Start Page | 561 |